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P78032

- TOP1_MYCPN

UniProt

P78032 - TOP1_MYCPN

Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
    Sitei33 – 331Interaction with DNAUniRule annotation
    Metal bindingi102 – 1021Magnesium 1; catalyticUniRule annotation
    Metal bindingi102 – 1021Magnesium 2UniRule annotation
    Metal bindingi104 – 1041Magnesium 2UniRule annotation
    Sitei160 – 1601Interaction with DNAUniRule annotation
    Sitei164 – 1641Interaction with DNAUniRule annotation
    Active sitei340 – 3401O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei342 – 3421Interaction with DNAUniRule annotation
    Sitei535 – 5351Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri624 – 65229C4-type 1Add
    BLAST
    Zinc fingeri673 – 70230C4-type 2Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMPNE272634:GJ6Z-268-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:MPN_261
    ORF Names:MP572
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 711711DNA topoisomerase 1PRO_0000145156Add
    BLAST

    Proteomic databases

    PaxDbiP78032.

    PTM databases

    PhosSiteiP1011993.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    IntActiP78032. 4 interactions.
    STRINGi272634.MPN261.

    Structurei

    3D structure databases

    ProteinModelPortaliP78032.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 134132ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni183 – 1886Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri624 – 65229C4-type 1Add
    BLAST
    Zinc fingeri673 – 70230C4-type 2Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    KOiK03168.
    OMAiVVECDKC.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P78032-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKNLVVIES PNKVKTLQKY LPNDFEIVST IGHIREMVHK NFGFNEADYS    50
    PVWEDWTKSK KKFSSLSFKG NLKGKKLLSK YDIIKSIKEK ASKATNIYLA 100
    TDPDREGEAI SWHVYDVLDE KDKSKCQRIT FNEITKNAVL DALKNPREID 150
    QSWVQSQFAR QILDRMIGFR LSRLLNNYLS AKSAGRVQSV ALRFLEEREQ 200
    EIRSFVPRFW WTLDVLLNPK AEGVREACAN RSIPIVLREI NPALRAGLKF 250
    EEEKSVSGID FLDEASAKKF GEQLKGVFEV YNIDETKHYS SSPNSAYTTA 300
    SLQKDAINKL GWSSKKVTLI AQHLYEGVSI NGEQTALISY PRTDSTRLSA 350
    QFQQSCKEYI LNHYGEKYLS NRIVSAKGKK GEKIIQDAHE AIHPTDINIT 400
    PEMVKNAIKK DEFLLYRLIW IRTVASLMAD CKKSHTHIRF INDGNKFYAS 450
    SKSLVFDGYR KIYEHFENKE SNDLYIDLDK IRVGDRFMAK DIKITARQTH 500
    PAARYTQASL IEALEKSNIG RPSTYNTMAS VNLDRGYASL NKHAFHVTQL 550
    GEQVNEELSK HFGKIINKEF TKNMEKSLDE IAENKKNYQE FLRDFWSNFK 600
    EEVKLAEGSI QRVKKEKEFV GRDCPSCASP LLYRYTKRGN EKFVGCSNFP 650
    NCKYNEFSQN KPNLTLEKLE ELCPECNSQL VKRRTKFNPN KTFVGCSNFP 700
    RCRYIKKDNA S 711
    Length:711
    Mass (Da):81,965
    Last modified:February 1, 1997 - v1
    Checksum:i16B863588B06582E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96220.1.
    PIRiS73898.
    RefSeqiNP_109949.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB96220; AAB96220; MPN_261.
    GeneIDi877375.
    KEGGimpn:MPN261.
    PATRICi20021847. VBIMycPne110_0280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96220.1 .
    PIRi S73898.
    RefSeqi NP_109949.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P78032.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P78032. 4 interactions.
    STRINGi 272634.MPN261.

    PTM databases

    PhosSitei P1011993.

    Proteomic databases

    PaxDbi P78032.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB96220 ; AAB96220 ; MPN_261 .
    GeneIDi 877375.
    KEGGi mpn:MPN261.
    PATRICi 20021847. VBIMycPne110_0280.

    Phylogenomic databases

    eggNOGi COG0551.
    KOi K03168.
    OMAi VVECDKC.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci MPNE272634:GJ6Z-268-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiTOP1_MYCPN
    AccessioniPrimary (citable) accession number: P78032
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3