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P78032

- TOP1_MYCPN

UniProt

P78032 - TOP1_MYCPN

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
Sitei33 – 331Interaction with DNAUniRule annotation
Metal bindingi102 – 1021Magnesium 1; catalyticUniRule annotation
Metal bindingi102 – 1021Magnesium 2UniRule annotation
Metal bindingi104 – 1041Magnesium 2UniRule annotation
Sitei160 – 1601Interaction with DNAUniRule annotation
Sitei164 – 1641Interaction with DNAUniRule annotation
Active sitei340 – 3401O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei342 – 3421Interaction with DNAUniRule annotation
Sitei535 – 5351Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri624 – 65229C4-type 1Add
BLAST
Zinc fingeri673 – 70230C4-type 2Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-268-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:MPN_261
ORF Names:MP572
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711DNA topoisomerase 1PRO_0000145156Add
BLAST

Proteomic databases

PaxDbiP78032.

PTM databases

PhosSiteiP1011993.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiP78032. 4 interactions.
STRINGi272634.MPN261.

Structurei

3D structure databases

ProteinModelPortaliP78032.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 134132ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni183 – 1886Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri624 – 65229C4-type 1Add
BLAST
Zinc fingeri673 – 70230C4-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78032-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKNLVVIES PNKVKTLQKY LPNDFEIVST IGHIREMVHK NFGFNEADYS
60 70 80 90 100
PVWEDWTKSK KKFSSLSFKG NLKGKKLLSK YDIIKSIKEK ASKATNIYLA
110 120 130 140 150
TDPDREGEAI SWHVYDVLDE KDKSKCQRIT FNEITKNAVL DALKNPREID
160 170 180 190 200
QSWVQSQFAR QILDRMIGFR LSRLLNNYLS AKSAGRVQSV ALRFLEEREQ
210 220 230 240 250
EIRSFVPRFW WTLDVLLNPK AEGVREACAN RSIPIVLREI NPALRAGLKF
260 270 280 290 300
EEEKSVSGID FLDEASAKKF GEQLKGVFEV YNIDETKHYS SSPNSAYTTA
310 320 330 340 350
SLQKDAINKL GWSSKKVTLI AQHLYEGVSI NGEQTALISY PRTDSTRLSA
360 370 380 390 400
QFQQSCKEYI LNHYGEKYLS NRIVSAKGKK GEKIIQDAHE AIHPTDINIT
410 420 430 440 450
PEMVKNAIKK DEFLLYRLIW IRTVASLMAD CKKSHTHIRF INDGNKFYAS
460 470 480 490 500
SKSLVFDGYR KIYEHFENKE SNDLYIDLDK IRVGDRFMAK DIKITARQTH
510 520 530 540 550
PAARYTQASL IEALEKSNIG RPSTYNTMAS VNLDRGYASL NKHAFHVTQL
560 570 580 590 600
GEQVNEELSK HFGKIINKEF TKNMEKSLDE IAENKKNYQE FLRDFWSNFK
610 620 630 640 650
EEVKLAEGSI QRVKKEKEFV GRDCPSCASP LLYRYTKRGN EKFVGCSNFP
660 670 680 690 700
NCKYNEFSQN KPNLTLEKLE ELCPECNSQL VKRRTKFNPN KTFVGCSNFP
710
RCRYIKKDNA S
Length:711
Mass (Da):81,965
Last modified:February 1, 1997 - v1
Checksum:i16B863588B06582E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB96220.1.
PIRiS73898.
RefSeqiNP_109949.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96220; AAB96220; MPN_261.
GeneIDi877375.
KEGGimpn:MPN261.
PATRICi20021847. VBIMycPne110_0280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB96220.1 .
PIRi S73898.
RefSeqi NP_109949.1. NC_000912.1.

3D structure databases

ProteinModelPortali P78032.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P78032. 4 interactions.
STRINGi 272634.MPN261.

PTM databases

PhosSitei P1011993.

Proteomic databases

PaxDbi P78032.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB96220 ; AAB96220 ; MPN_261 .
GeneIDi 877375.
KEGGi mpn:MPN261.
PATRICi 20021847. VBIMycPne110_0280.

Phylogenomic databases

eggNOGi COG0551.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci MPNE272634:GJ6Z-268-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiTOP1_MYCPN
AccessioniPrimary (citable) accession number: P78032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3