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P78032 (TOP1_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:MPN_261
ORF Names:MP572
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145156

Regions

Domain3 – 134132Toprim
Zinc finger624 – 65229C4-type 1 HAMAP-Rule MF_00952
Zinc finger673 – 70230C4-type 2 HAMAP-Rule MF_00952
Region183 – 1886Interaction with DNA By similarity

Sites

Active site3401O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding1021Magnesium 1; catalytic By similarity
Metal binding1021Magnesium 2 By similarity
Metal binding1041Magnesium 2 By similarity
Site331Interaction with DNA By similarity
Site1601Interaction with DNA By similarity
Site1641Interaction with DNA By similarity
Site3421Interaction with DNA By similarity
Site5351Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P78032 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 16B863588B06582E

FASTA71181,965
        10         20         30         40         50         60 
MSKNLVVIES PNKVKTLQKY LPNDFEIVST IGHIREMVHK NFGFNEADYS PVWEDWTKSK 

        70         80         90        100        110        120 
KKFSSLSFKG NLKGKKLLSK YDIIKSIKEK ASKATNIYLA TDPDREGEAI SWHVYDVLDE 

       130        140        150        160        170        180 
KDKSKCQRIT FNEITKNAVL DALKNPREID QSWVQSQFAR QILDRMIGFR LSRLLNNYLS 

       190        200        210        220        230        240 
AKSAGRVQSV ALRFLEEREQ EIRSFVPRFW WTLDVLLNPK AEGVREACAN RSIPIVLREI 

       250        260        270        280        290        300 
NPALRAGLKF EEEKSVSGID FLDEASAKKF GEQLKGVFEV YNIDETKHYS SSPNSAYTTA 

       310        320        330        340        350        360 
SLQKDAINKL GWSSKKVTLI AQHLYEGVSI NGEQTALISY PRTDSTRLSA QFQQSCKEYI 

       370        380        390        400        410        420 
LNHYGEKYLS NRIVSAKGKK GEKIIQDAHE AIHPTDINIT PEMVKNAIKK DEFLLYRLIW 

       430        440        450        460        470        480 
IRTVASLMAD CKKSHTHIRF INDGNKFYAS SKSLVFDGYR KIYEHFENKE SNDLYIDLDK 

       490        500        510        520        530        540 
IRVGDRFMAK DIKITARQTH PAARYTQASL IEALEKSNIG RPSTYNTMAS VNLDRGYASL 

       550        560        570        580        590        600 
NKHAFHVTQL GEQVNEELSK HFGKIINKEF TKNMEKSLDE IAENKKNYQE FLRDFWSNFK 

       610        620        630        640        650        660 
EEVKLAEGSI QRVKKEKEFV GRDCPSCASP LLYRYTKRGN EKFVGCSNFP NCKYNEFSQN 

       670        680        690        700        710 
KPNLTLEKLE ELCPECNSQL VKRRTKFNPN KTFVGCSNFP RCRYIKKDNA S 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96220.1.
PIRS73898.
RefSeqNP_109949.1. NC_000912.1.

3D structure databases

ProteinModelPortalP78032.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP78032. 4 interactions.
STRING272634.MPN261.

PTM databases

PhosSiteP1011993.

Proteomic databases

PaxDbP78032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96220; AAB96220; MPN_261.
GeneID877375.
KEGGmpn:MPN261.
PATRIC20021847. VBIMycPne110_0280.

Phylogenomic databases

eggNOGCOG0551.
KOK03168.
OMAMVYKMGR.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK05582.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-268-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_MYCPN
AccessionPrimary (citable) accession number: P78032
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 19, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names