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P78031 (KPYK_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
Ordered Locus Names:MPN_303
ORF Names:MP533
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Enzyme regulation

Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508Pyruvate kinase
PRO_0000112081

Sites

Metal binding581Potassium By similarity
Metal binding601Potassium By similarity
Metal binding901Potassium By similarity
Metal binding911Potassium; via carbonyl oxygen By similarity
Metal binding2511Magnesium By similarity
Metal binding2751Magnesium By similarity
Binding site561Substrate By similarity
Binding site2741Substrate; via amide nitrogen By similarity
Binding site2751Substrate; via amide nitrogen By similarity
Binding site3071Substrate By similarity
Site2491Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
P78031 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 011D97377F8BB527

FASTA50857,268
        10         20         30         40         50         60 
MIHHLKRTKI IATCGPALTK KLWTLAMLDD PAYAAMKAEA YANIENIIKN GVTVIRLNFS 

        70         80         90        100        110        120 
HGNHEEQAVR IKIVRDVAKK LNLPVSIMLD TNGPEIRVFE TAPEGLKILK DSEVVINTTT 

       130        140        150        160        170        180 
KEVAKNNQFS VSDASGTYNM VNDVKVGQKI LVDDGKLSLV VKRIDTKNNQ VICVAQNDHT 

       190        200        210        220        230        240 
IFTKKRLNLP NADYSIPFLS AKDLRDIDFG LTHQIDYIAA SFVNTTENIK QLRDYLASKN 

       250        260        270        280        290        300 
AKHVKLIAKI ESNHALNNID GIIKASDGIM VARGDLGLEI PYYKVPYWQR YMIKACRFFN 

       310        320        330        340        350        360 
KRVITATQML DSLEKNIQPT RAEVTDVYFA VDRGNDATML SGETANGAFP LNAVYVMKMI 

       370        380        390        400        410        420 
DKQSETFFDY QYNLNYYMAN SKARHSEFWK QVVLPLAQKT APKRKLINSD FKYDFVVHAT 

       430        440        450        460        470        480 
NNLNEIYALS NARLAAAVII LTNDPQVYTG HGVDYGIFPY LIDQKPQSLS KAEFKSLANV 

       490        500 
AIKHYQQHGE ISQLKQCLGV FHNKIISL 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96181.1.
PIRS73859.
RefSeqNP_109991.1. NC_000912.1.

3D structure databases

ProteinModelPortalP78031.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP78031. 2 interactions.
STRING272634.MPN303.

Proteomic databases

PaxDbP78031.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96181; AAB96181; MPN_303.
GeneID877217.
KEGGmpn:MPN303.
PATRIC20021941. VBIMycPne110_0327.

Phylogenomic databases

eggNOGCOG0469.
KOK00873.
OMAGDETMIS.
OrthoDBEOG6GBMB0.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-310-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_MYCPN
AccessionPrimary (citable) accession number: P78031
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names