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P78029 (TYSY_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:MPN_320
ORF Names:MP516
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Sequence caution

The sequence AAB96164.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 287287Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140987

Sites

Active site1701 By similarity

Experimental info

Sequence conflict204 – 2129SYSLLVYLV → FLLSYGA Ref.2

Sequences

Sequence LengthMass (Da)Tools
P78029 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: DAD2E84FCD9C5EDE

FASTA28733,591
        10         20         30         40         50         60 
MQQYLDLARY VLEHGKYRPN RTDTAGIGVF GYQMHFDISK HFPLLTTKKV HWKSIVHELL 

        70         80         90        100        110        120 
WFIKGDTNIK YLVDNKVNIW NEWPYESFKK SPHFNGESQK EFIERIRQDA KFAQQFGNLG 

       130        140        150        160        170        180 
PVYGKQWRDF NGVDQLKKVI AQIKVNPFSR RLIVSSWNPN EVDQMLLPPC HSLYQFYVQD 

       190        200        210        220        230        240 
GQLSCQLYQR SGDIFLGIPF NIASYSLLVY LVAKETNLKP GSFVHTIGDA HIYENHLEQI 

       250        260        270        280 
KLQLTRQPKP LPKVVLKSDK SIFDYQFDDI ELVDYDHHPT IKGEVAV 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.
[2]"Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins."
Proft T., Herrmann R.
Mol. Microbiol. 13:337-348(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-212.
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96164.1. Different initiation.
Z32654 Genomic DNA. Translation: CAA83575.1.
PIRS73842.
RefSeqNP_110008.2. NC_000912.1.

3D structure databases

ProteinModelPortalP78029.
SMRP78029. Positions 2-287.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP78029. 3 interactions.
STRING272634.MPN320.

Proteomic databases

PaxDbP78029.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96164; AAB96164; MPN_320.
GeneID876944.
KEGGmpn:MPN320.
PATRIC20021994. VBIMycPne110_0344.

Phylogenomic databases

eggNOGCOG0207.
KOK00560.
OMANLGPVYG.
OrthoDBEOG6K6V53.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-337-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_MYCPN
AccessionPrimary (citable) accession number: P78029
Secondary accession number(s): Q50354
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names