P78029 (TYSY_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	MPN_320
ORF Names:	MP516

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00008
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
Sequence caution	The sequence AAB96164.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000140987
Sites
	Active site	170	1	By similarity
Experimental info
	Sequence conflict	204 – 212	9	SYSLLVYLV → FLLSYGA Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P78029 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: DAD2E84FCD9C5EDE	FASTA	287	33,591
	10 20 30 40 50 60 MQQYLDLARY VLEHGKYRPN RTDTAGIGVF GYQMHFDISK HFPLLTTKKV HWKSIVHELL 70 80 90 100 110 120 WFIKGDTNIK YLVDNKVNIW NEWPYESFKK SPHFNGESQK EFIERIRQDA KFAQQFGNLG 130 140 150 160 170 180 PVYGKQWRDF NGVDQLKKVI AQIKVNPFSR RLIVSSWNPN EVDQMLLPPC HSLYQFYVQD 190 200 210 220 230 240 GQLSCQLYQR SGDIFLGIPF NIASYSLLVY LVAKETNLKP GSFVHTIGDA HIYENHLEQI 250 260 270 280 KLQLTRQPKP LPKVVLKSDK SIFDYQFDDI ELVDYDHHPT IKGEVAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins." Proft T., Herrmann R. Mol. Microbiol. 13:337-348(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-212. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96164.1. Different initiation. Z32654 Genomic DNA. Translation: CAA83575.1.
PIR	S73842.
RefSeq	NP_110008.2. NC_000912.1.
3D structure databases
ProteinModelPortal	P78029.
SMR	P78029. Positions 2-287.
ModBase	Search...
Protein-protein interaction databases
IntAct	P78029. 3 interactions.
STRING	272634.MPN320.
Proteomic databases
PaxDb	P78029.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96164; AAB96164; MPN_320.
GeneID	876944.
KEGG	mpn:MPN320.
PATRIC	20021994. VBIMycPne110_0344.
Phylogenomic databases
eggNOG	COG0207.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-337-MONOMER.
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_MYCPN

Accession

Primary (citable) accession number: P78029
Secondary accession number(s): Q50354

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program