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Protein

Thymidylate synthase

Gene

thyA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701UniRule annotation

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dTMP biosynthetic process Source: UniProtKB-HAMAP
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-337-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:MPN_320
ORF Names:MP516
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Thymidylate synthasePRO_0000140987Add
BLAST

Proteomic databases

PaxDbiP78029.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP78029. 3 interactions.
STRINGi272634.MPN320.

Structurei

3D structure databases

ProteinModelPortaliP78029.
SMRiP78029. Positions 2-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78029-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQYLDLARY VLEHGKYRPN RTDTAGIGVF GYQMHFDISK HFPLLTTKKV
60 70 80 90 100
HWKSIVHELL WFIKGDTNIK YLVDNKVNIW NEWPYESFKK SPHFNGESQK
110 120 130 140 150
EFIERIRQDA KFAQQFGNLG PVYGKQWRDF NGVDQLKKVI AQIKVNPFSR
160 170 180 190 200
RLIVSSWNPN EVDQMLLPPC HSLYQFYVQD GQLSCQLYQR SGDIFLGIPF
210 220 230 240 250
NIASYSLLVY LVAKETNLKP GSFVHTIGDA HIYENHLEQI KLQLTRQPKP
260 270 280
LPKVVLKSDK SIFDYQFDDI ELVDYDHHPT IKGEVAV
Length:287
Mass (Da):33,591
Last modified:November 1, 1997 - v2
Checksum:iDAD2E84FCD9C5EDE
GO

Sequence cautioni

The sequence AAB96164.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti204 – 2129SYSLLVYLV → FLLSYGA (PubMed:7984111).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96164.1. Different initiation.
Z32654 Genomic DNA. Translation: CAA83575.1.
PIRiS73842.
RefSeqiNP_110008.2. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96164; AAB96164; MPN_320.
GeneIDi876944.
KEGGimpn:MPN320.
PATRICi20021994. VBIMycPne110_0344.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96164.1. Different initiation.
Z32654 Genomic DNA. Translation: CAA83575.1.
PIRiS73842.
RefSeqiNP_110008.2. NC_000912.1.

3D structure databases

ProteinModelPortaliP78029.
SMRiP78029. Positions 2-287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP78029. 3 interactions.
STRINGi272634.MPN320.

Proteomic databases

PaxDbiP78029.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96164; AAB96164; MPN_320.
GeneIDi876944.
KEGGimpn:MPN320.
PATRICi20021994. VBIMycPne110_0344.

Phylogenomic databases

eggNOGiCOG0207.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciMPNE272634:GJ6Z-337-MONOMER.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.
  2. "Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins."
    Proft T., Herrmann R.
    Mol. Microbiol. 13:337-348(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-212.
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiTYSY_MYCPN
AccessioniPrimary (citable) accession number: P78029
Secondary accession number(s): Q50354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.