folA

Functionⁱ

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityⁱ

5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayⁱ: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:

Dihydrofolate reductase (folA)

This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	27	SubstrateBy similarity	1
Binding siteⁱ	32	SubstrateBy similarity	1
Binding siteⁱ	57	SubstrateBy similarity	1
Binding siteⁱ	120	SubstrateBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	6 – 7	NADPBy similarity	2
Nucleotide bindingⁱ	14 – 19	NADPBy similarity	6
Nucleotide bindingⁱ	43 – 46	NADPBy similarity	4
Nucleotide bindingⁱ	62 – 65	NADPBy similarity	4
Nucleotide bindingⁱ	101 – 106	NADPBy similarity	6

GO - Molecular functionⁱ

dihydrofolate reductase activity Source: UniProtKB-EC
NADP binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glycine biosynthetic process Source: InterPro
one-carbon metabolic process Source: UniProtKB-KW
tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	One-carbon metabolism
Ligand	NADP

Enzyme and pathway databases

BioCycⁱ	MetaCyc:MONOMER-579. MPNE272634:G1GJ3-512-MONOMER.
UniPathwayⁱ	UPA00077; UER00158.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Dihydrofolate reductase (EC:1.5.1.3)
Gene namesⁱ	Name:folA Synonyms:dhfR Ordered Locus Names:MPN_321 ORF Names:MP515
Organismⁱ	Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifierⁱ	272634 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae
Proteomesⁱ	UP000000808 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000186396	1 – 160	Dihydrofolate reductaseAdd BLAST		160

Interactionⁱ

Protein-protein interaction databases

IntActⁱ	P78028. 5 interactors.

IntActⁱ

P78028. 5 interactors.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P78028.
SMRⁱ	P78028.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 160	DHFRPROSITE-ProRule annotationAdd BLAST		160

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	5 – 7	Substrate bindingBy similarity		3

Sequence similaritiesⁱ

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K00287.
OMAⁱ	EECFVIG.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00209. DHFR. 1 hit.
Gene3Dⁱ	3.40.430.10. 1 hit.
InterProⁱ	View protein in InterPro IPR012259. DHFR. IPR024072. DHFR-like_dom_sf. IPR017925. DHFR_CS. IPR001796. DHFR_dom.
PANTHERⁱ	PTHR22778:SF16. PTHR22778:SF16. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00186. DHFR_1. 1 hit.
SUPFAMⁱ	SSF53597. SSF53597. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P78028-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVKAIWAMDQ NGLIGNGNSL PWRIKAELQH FRQTTLHQDV LMGSATYLSL 
        60         70         80         90        100
PPVFSERNVY ILTRNLNFNP PDKGCLTKVI HEYENFIQPY LHHPDKHLYI 
       110        120        130        140        150
CGGAQVYEQL IPRCDALIVS TIFGKYTGDK YLKVDFSPFE LTKEISFAEF 
       160
KVAYYHKIAR

Length:160

Mass (Da):18,511

Last modified:February 1, 1997 - v1

Checksum:ⁱ87C323433A3FABA4

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00089 Genomic DNA. Translation: AAB96163.1.
PIRⁱ	S73841.
NCBI Reference Sequences More...RefSeqⁱ	NP_110009.1. NC_000912.1. WP_010874677.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAB96163; AAB96163; MPN_321.
GeneIDⁱ	876943.
KEGGⁱ	mpn:MPN321.
PATRICⁱ	fig\|272634.6.peg.345.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P78028	A0A0H3DKL6	Mycoplasma pneumoniae (strain ATCC 15531 / DSM 22911 / NBRC 14401 / NCTC 10119 / FH)	160	UniRef100_P78028	Cluster: Dihydrofolate reductase	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P78028	A0A0H3DKL6 UPI00071B3686 UPI000A29ED25	Mycoplasma pneumoniae (strain ATCC 15531 / DSM 22911 / NBRC 14401 / NCTC 10119 / FH) Mycoplasma pneumoniae	160	UniRef90_P78028	Cluster: Dihydrofolate reductase	4

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P78028	P47470 A0A0H3DKL6 UPI00071B3686 UPI000A29ED25	Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) Mycoplasma pneumoniae (strain ATCC 15531 / DSM 22911 / NBRC 14401 / NCTC 10119 / FH) Mycoplasma pneumoniae	160	UniRef50_P47470	Cluster: Dihydrofolate reductase	5

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00089 Genomic DNA. Translation: AAB96163.1.
PIRⁱ	S73841.
NCBI Reference Sequences More...RefSeqⁱ	NP_110009.1. NC_000912.1. WP_010874677.1. NC_000912.1.

3D structure databases

ProteinModelPortalⁱ	P78028.
SMRⁱ	P78028.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P78028. 5 interactors.

IntActⁱ

P78028. 5 interactors.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAB96163; AAB96163; MPN_321.
GeneIDⁱ	876943.
KEGGⁱ	mpn:MPN321.
PATRICⁱ	fig\|272634.6.peg.345.

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K00287.
OMAⁱ	EECFVIG.

Enzyme and pathway databases

UniPathwayⁱ	UPA00077; UER00158.
BioCycⁱ	MetaCyc:MONOMER-579. MPNE272634:G1GJ3-512-MONOMER.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00209. DHFR. 1 hit.
Gene3Dⁱ	3.40.430.10. 1 hit.
InterProⁱ	View protein in InterPro IPR012259. DHFR. IPR024072. DHFR-like_dom_sf. IPR017925. DHFR_CS. IPR001796. DHFR_dom.
PANTHERⁱ	PTHR22778:SF16. PTHR22778:SF16. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00186. DHFR_1. 1 hit.
SUPFAMⁱ	SSF53597. SSF53597. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DYR_MYCPN
Accessionⁱ	P78028Primary (citable) accession number: P78028
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	February 1, 1997
	Last modified:	March 28, 2018
	This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P78028 (DYR_MYCPN)

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Dihydrofolate reductase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tetrahydrofolate biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: tetrahydrofolate biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ