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P78028 (DYR_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:folA
Synonyms:dhfR
Ordered Locus Names:MPN_321
ORF Names:MP515
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length160 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 160160Dihydrofolate reductase
PRO_0000186396

Regions

Domain1 – 160160DHFR
Nucleotide binding6 – 72NADP By similarity
Nucleotide binding14 – 196NADP By similarity
Nucleotide binding43 – 464NADP By similarity
Nucleotide binding62 – 654NADP By similarity
Nucleotide binding101 – 1066NADP By similarity
Region5 – 73Substrate binding By similarity

Sites

Binding site271Substrate By similarity
Binding site321Substrate By similarity
Binding site571Substrate By similarity
Binding site1201Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P78028 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 87C323433A3FABA4

FASTA16018,511
        10         20         30         40         50         60 
MVKAIWAMDQ NGLIGNGNSL PWRIKAELQH FRQTTLHQDV LMGSATYLSL PPVFSERNVY 

        70         80         90        100        110        120 
ILTRNLNFNP PDKGCLTKVI HEYENFIQPY LHHPDKHLYI CGGAQVYEQL IPRCDALIVS 

       130        140        150        160 
TIFGKYTGDK YLKVDFSPFE LTKEISFAEF KVAYYHKIAR 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96163.1.
PIRS73841.
RefSeqNP_110009.1. NC_000912.1.

3D structure databases

ProteinModelPortalP78028.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP78028. 5 interactions.
STRING272634.MPN321.

Proteomic databases

PaxDbP78028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96163; AAB96163; MPN_321.
GeneID876943.
KEGGmpn:MPN321.
PATRIC20021996. VBIMycPne110_0345.

Phylogenomic databases

eggNOGCOG0262.
KOK00287.
OMAKNKYPYT.
OrthoDBEOG6KT2V2.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-579.
MPNE272634:GJ6Z-338-MONOMER.
UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_MYCPN
AccessionPrimary (citable) accession number: P78028
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names