P78028 (DYR_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrofolate reductase
EC=1.5.1.3

Gene names

Name:	folA
Synonyms:	dhfR
Ordered Locus Names:	MPN_321
ORF Names:	MP515

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: UniProtKB-EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 160	160	Dihydrofolate reductase		PRO_0000186396
Regions
	Domain	1 – 160	160	DHFR
	Nucleotide binding	6 – 7	2	NADP By similarity
	Nucleotide binding	14 – 19	6	NADP By similarity
	Nucleotide binding	43 – 46	4	NADP By similarity
	Nucleotide binding	62 – 65	4	NADP By similarity
	Nucleotide binding	101 – 106	6	NADP By similarity
	Region	5 – 7	3	Substrate binding By similarity
Sites
	Binding site	27	1	Substrate By similarity
	Binding site	32	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	120	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P78028 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 87C323433A3FABA4	FASTA	160	18,511
	10 20 30 40 50 60 MVKAIWAMDQ NGLIGNGNSL PWRIKAELQH FRQTTLHQDV LMGSATYLSL PPVFSERNVY 70 80 90 100 110 120 ILTRNLNFNP PDKGCLTKVI HEYENFIQPY LHHPDKHLYI CGGAQVYEQL IPRCDALIVS 130 140 150 160 TIFGKYTGDK YLKVDFSPFE LTKEISFAEF KVAYYHKIAR

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96163.1.
PIR	S73841.
RefSeq	NP_110009.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P78028.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
IntAct	P78028. 5 interactions.
STRING	272634.MPN321.
Proteomic databases
PaxDb	P78028.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96163; AAB96163; MPN_321.
GeneID	876943.
KEGG	mpn:MPN321.
PATRIC	20021996. VBIMycPne110_0345.
Phylogenomic databases
eggNOG	COG0262.
KO	K00287.
OMA	KNKYPYT.
OrthoDB	EOG6KT2V2.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-579. MPNE272634:GJ6Z-338-MONOMER.
UniPathway	UPA00077; UER00158.
Family and domain databases
Gene3D	3.40.430.10. 1 hit.
InterPro	IPR012259. DHFR. IPR024072. DHFR-like_dom. IPR017925. DHFR_CS. IPR001796. DHFR_dom. [Graphical view]
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
SUPFAM	SSF53597. SSF53597. 1 hit.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_MYCPN

Accession

Primary (citable) accession number: P78028

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program