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P78028

- DYR_MYCPN

UniProt

P78028 - DYR_MYCPN

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Protein
Dihydrofolate reductase
Gene
folA, dhfR, MPN_321, MP515
Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271Substrate By similarity
Binding sitei32 – 321Substrate By similarity
Binding sitei57 – 571Substrate By similarity
Binding sitei120 – 1201Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 72NADP By similarity
Nucleotide bindingi14 – 196NADP By similarity
Nucleotide bindingi43 – 464NADP By similarity
Nucleotide bindingi62 – 654NADP By similarity
Nucleotide bindingi101 – 1066NADP By similarity

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. dihydrofolate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-579.
MPNE272634:GJ6Z-338-MONOMER.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:dhfR
Ordered Locus Names:MPN_321
ORF Names:MP515
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160Dihydrofolate reductase
PRO_0000186396Add
BLAST

Proteomic databases

PaxDbiP78028.

Interactioni

Protein-protein interaction databases

IntActiP78028. 5 interactions.
STRINGi272634.MPN321.

Structurei

3D structure databases

ProteinModelPortaliP78028.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 160160DHFR
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 73Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0262.
KOiK00287.
OMAiKNKYPYT.
OrthoDBiEOG6KT2V2.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78028-1 [UniParc]FASTAAdd to Basket

« Hide

MVKAIWAMDQ NGLIGNGNSL PWRIKAELQH FRQTTLHQDV LMGSATYLSL    50
PPVFSERNVY ILTRNLNFNP PDKGCLTKVI HEYENFIQPY LHHPDKHLYI 100
CGGAQVYEQL IPRCDALIVS TIFGKYTGDK YLKVDFSPFE LTKEISFAEF 150
KVAYYHKIAR 160
Length:160
Mass (Da):18,511
Last modified:February 1, 1997 - v1
Checksum:i87C323433A3FABA4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB96163.1.
PIRiS73841.
RefSeqiNP_110009.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96163; AAB96163; MPN_321.
GeneIDi876943.
KEGGimpn:MPN321.
PATRICi20021996. VBIMycPne110_0345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB96163.1 .
PIRi S73841.
RefSeqi NP_110009.1. NC_000912.1.

3D structure databases

ProteinModelPortali P78028.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P78028. 5 interactions.
STRINGi 272634.MPN321.

Proteomic databases

PaxDbi P78028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB96163 ; AAB96163 ; MPN_321 .
GeneIDi 876943.
KEGGi mpn:MPN321.
PATRICi 20021996. VBIMycPne110_0345.

Phylogenomic databases

eggNOGi COG0262.
KOi K00287.
OMAi KNKYPYT.
OrthoDBi EOG6KT2V2.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .
BioCyci MetaCyc:MONOMER-579.
MPNE272634:GJ6Z-338-MONOMER.

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiDYR_MYCPN
AccessioniPrimary (citable) accession number: P78028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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