UniProtKB - P78028 (DYR_MYCPN)
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Protein
Dihydrofolate reductase
Gene
folA
Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Functioni
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity
Catalytic activityi
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation
: tetrahydrofolate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.Proteins known to be involved in this subpathway in this organism are:
- Dihydrofolate reductase (folA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 27 | SubstrateBy similarity | 1 | |
Binding sitei | 32 | SubstrateBy similarity | 1 | |
Binding sitei | 57 | SubstrateBy similarity | 1 | |
Binding sitei | 120 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 6 – 7 | NADPBy similarity | 2 | |
Nucleotide bindingi | 14 – 19 | NADPBy similarity | 6 | |
Nucleotide bindingi | 43 – 46 | NADPBy similarity | 4 | |
Nucleotide bindingi | 62 – 65 | NADPBy similarity | 4 | |
Nucleotide bindingi | 101 – 106 | NADPBy similarity | 6 |
GO - Molecular functioni
- dihydrofolate reductase activity Source: UniProtKB-EC
- NADP binding Source: InterPro
GO - Biological processi
- glycine biosynthetic process Source: InterPro
- one-carbon metabolic process Source: UniProtKB-KW
- tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | One-carbon metabolism |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-579. MPNE272634:G1GJ3-512-MONOMER. |
UniPathwayi | UPA00077; UER00158. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:folA Synonyms:dhfR Ordered Locus Names:MPN_321 ORF Names:MP515 |
Organismi | Mycoplasma pneumoniae (strain ATCC 29342 / M129) |
Taxonomic identifieri | 272634 [NCBI] |
Taxonomic lineagei | Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186396 | 1 – 160 | Dihydrofolate reductaseAdd BLAST | 160 |
Structurei
3D structure databases
ProteinModelPortali | P78028. |
SMRi | P78028. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 160 | DHFRPROSITE-ProRule annotationAdd BLAST | 160 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 5 – 7 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the dihydrofolate reductase family.Curated
Phylogenomic databases
KOi | K00287. |
OMAi | EECFVIG. |
Family and domain databases
CDDi | cd00209. DHFR. 1 hit. |
Gene3Di | 3.40.430.10. 1 hit. |
InterProi | View protein in InterPro IPR012259. DHFR. IPR024072. DHFR-like_dom_sf. IPR017925. DHFR_CS. IPR001796. DHFR_dom. |
PANTHERi | PTHR22778:SF16. PTHR22778:SF16. 1 hit. |
Pfami | View protein in Pfam PF00186. DHFR_1. 1 hit. |
SUPFAMi | SSF53597. SSF53597. 1 hit. |
PROSITEi | View protein in PROSITE PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. |
i Sequence
Sequence statusi: Complete.
P78028-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVKAIWAMDQ NGLIGNGNSL PWRIKAELQH FRQTTLHQDV LMGSATYLSL
60 70 80 90 100
PPVFSERNVY ILTRNLNFNP PDKGCLTKVI HEYENFIQPY LHHPDKHLYI
110 120 130 140 150
CGGAQVYEQL IPRCDALIVS TIFGKYTGDK YLKVDFSPFE LTKEISFAEF
160
KVAYYHKIAR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00089 Genomic DNA. Translation: AAB96163.1. |
PIRi | S73841. |
RefSeqi | NP_110009.1. NC_000912.1. WP_010874677.1. NC_000912.1. |
Genome annotation databases
EnsemblBacteriai | AAB96163; AAB96163; MPN_321. |
GeneIDi | 876943. |
KEGGi | mpn:MPN321. |
PATRICi | fig|272634.6.peg.345. |
Similar proteinsi
Entry informationi
Entry namei | DYR_MYCPN | |
Accessioni | P78028Primary (citable) accession number: P78028 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | March 28, 2018 | |
This is version 97 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |