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P78028

- DYR_MYCPN

UniProt

P78028 - DYR_MYCPN

Protein

Dihydrofolate reductase

Gene

folA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei27 – 271SubstrateBy similarity
    Binding sitei32 – 321SubstrateBy similarity
    Binding sitei57 – 571SubstrateBy similarity
    Binding sitei120 – 1201SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi6 – 72NADPBy similarity
    Nucleotide bindingi14 – 196NADPBy similarity
    Nucleotide bindingi43 – 464NADPBy similarity
    Nucleotide bindingi62 – 654NADPBy similarity
    Nucleotide bindingi101 – 1066NADPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process Source: InterPro
    2. nucleotide biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-579.
    MPNE272634:GJ6Z-338-MONOMER.
    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:folA
    Synonyms:dhfR
    Ordered Locus Names:MPN_321
    ORF Names:MP515
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 160160Dihydrofolate reductasePRO_0000186396Add
    BLAST

    Proteomic databases

    PaxDbiP78028.

    Interactioni

    Protein-protein interaction databases

    IntActiP78028. 5 interactions.
    STRINGi272634.MPN321.

    Structurei

    3D structure databases

    ProteinModelPortaliP78028.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 160160DHFRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni5 – 73Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0262.
    KOiK00287.
    OMAiKNKYPYT.
    OrthoDBiEOG6KT2V2.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P78028-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKAIWAMDQ NGLIGNGNSL PWRIKAELQH FRQTTLHQDV LMGSATYLSL    50
    PPVFSERNVY ILTRNLNFNP PDKGCLTKVI HEYENFIQPY LHHPDKHLYI 100
    CGGAQVYEQL IPRCDALIVS TIFGKYTGDK YLKVDFSPFE LTKEISFAEF 150
    KVAYYHKIAR 160
    Length:160
    Mass (Da):18,511
    Last modified:February 1, 1997 - v1
    Checksum:i87C323433A3FABA4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96163.1.
    PIRiS73841.
    RefSeqiNP_110009.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB96163; AAB96163; MPN_321.
    GeneIDi876943.
    KEGGimpn:MPN321.
    PATRICi20021996. VBIMycPne110_0345.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96163.1 .
    PIRi S73841.
    RefSeqi NP_110009.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P78028.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P78028. 5 interactions.
    STRINGi 272634.MPN321.

    Proteomic databases

    PaxDbi P78028.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB96163 ; AAB96163 ; MPN_321 .
    GeneIDi 876943.
    KEGGi mpn:MPN321.
    PATRICi 20021996. VBIMycPne110_0345.

    Phylogenomic databases

    eggNOGi COG0262.
    KOi K00287.
    OMAi KNKYPYT.
    OrthoDBi EOG6KT2V2.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .
    BioCyci MetaCyc:MONOMER-579.
    MPNE272634:GJ6Z-338-MONOMER.

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiDYR_MYCPN
    AccessioniPrimary (citable) accession number: P78028
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3