Reviewed, UniProtKB/Swiss-Prot P78027 (RIR1_MYCPN)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase

Gene names

Name:	nrdE
Ordered Locus Names:	MPN_324
ORF Names:	MP512

Organism

Mycoplasma pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

2104 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Ribonucleoside-diphosphate reductase subunit alpha		PRO_0000187218
Sites
	Active site	176	1	Hydrogen atom transfer By similarity
	Active site	384	1	Proton acceptor By similarity
	Active site	386	1	Proton acceptor By similarity
	Active site	388	1	Proton acceptor By similarity
	Active site	413	1	Hydrogen atom transfer By similarity
	Active site	694	1	Electron transfer By similarity
	Active site	695	1	Electron transfer By similarity
	Site	183	1	Allosteric effector binding By similarity
	Site	213	1	Allosteric effector binding By similarity
	Site	716	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	719	1	Interacts with thioredoxin/glutaredoxin By similarity
Amino acid modifications
	Disulfide bond	176 ↔ 413		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P78027-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 93CAEA95A2A9E647	FASTA	721	82,375
	10 20 30 40 50 60 MSVKEKIPAF NTQEDLESYI SLNAYTKVYG DFKMDLHAVE AYIQEHVKPK TKVFHSTKER 70 80 90 100 110 120 LDFLVKNDYY DENIINMYSF EQFEEITRKA YAYRFRYANF MGAFKFYNAY ALKTFDGKWY 130 140 150 160 170 180 LENYEDRVVM NVLFLANGNY NKALKLLKQI ITNRFQPATP TFLNAGRKKR GEFVSCYLLR 190 200 210 220 230 240 IEDNMESIGR AITTTLQLSK RDGGVALLLT NIRESGAPIK KIENQSSGII PIMKLLEDSF 250 260 270 280 290 300 SYANQLGQRQ GAGAVYLHAH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFTLAKN 310 320 330 340 350 360 NEEMALFSPY DVYEEYGKPL SDISVTEMYY ELLANQRIKK TFINARKFFQ TVAELHFESG 370 380 390 400 410 420 YPYILFDDTV NRRNAHPNRI VMSNLCSEIV QPSTPSEFHH DLAFKKVGND ISCNLGSLNI 430 440 450 460 470 480 AKAMESGPEF SELVKLAIES LDLVSRVSNL ETAPSIQKGN SENHALGLGA MNLHGFLATN 490 500 510 520 530 540 QIYYNSPEAI DFTNIFFYTV AYHAFKASSE LALEKGKFKN FENTKFADGS YFDKYIKVEP 550 560 570 580 590 600 DFWTPKTERV KALFQKYQVE IPTRENWKEL ALNIQKNGLA NSHLLAIAPT GSISYLSSCT 610 620 630 640 650 660 PSLQPVVSPV EVRKEGRLGR IYVPAYQLNK DSYPFYKDGA YELGPEPIIN IAAAAQQHVD 670 680 690 700 710 720 QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ EVLEDSEDHT IQMQQCEACV I

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00089 Genomic DNA. Translation: AAB96160.1.
PIR	S73838.
RefSeq	NP_110012.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	876946.
GenomeReviews	Gene locus MPN_324 in contig U00089_GR.
KEGG	mpn:MPN324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P78027.
OMA	HIPTQDD.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-577. MPNE272634:MPN324-MON.
BRENDA	1.17.4.1. 39500.
Family and domain databases
InterPro	IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. IPR013554. Ribonucl_Rdtase_N. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. PF08343. RNR_N. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_MYCPN

Accession

Primary (citable) accession number: P78027

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)