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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdE

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateBy similarity1
Sitei176Important for hydrogen atom transferBy similarity1
Sitei183Allosteric effector bindingBy similarity1
Binding sitei204Substrate; via amide nitrogenBy similarity1
Sitei213Allosteric effector bindingBy similarity1
Active sitei384Proton acceptorBy similarity1
Active sitei386Cysteine radical intermediateBy similarity1
Active sitei388Proton acceptorBy similarity1
Sitei413Important for hydrogen atom transferBy similarity1
Sitei694Important for electron transferBy similarity1
Sitei695Important for electron transferBy similarity1
Sitei716Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei719Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPNE272634:G1GJ3-515-MONOMER
UniPathwayiUPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdE
Ordered Locus Names:MPN_324
ORF Names:MP512
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872181 – 721Ribonucleoside-diphosphate reductase subunit alphaAdd BLAST721

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi176 ↔ 413Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP78027

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

IntActiP78027, 7 interactors

Structurei

3D structure databases

ProteinModelPortaliP78027
SMRiP78027
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni175 – 176Substrate bindingBy similarity2
Regioni384 – 388Substrate bindingBy similarity5
Regioni589 – 593Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

KOiK00525
OMAiDRYFLHI

Family and domain databases

InterProiView protein in InterPro
IPR013346 NrdE_NrdA
IPR026459 RNR_1b_NrdE
IPR000788 RNR_lg_C
IPR013509 RNR_lsu_N
IPR013554 RNR_N
IPR008926 RNR_R1-su_N
PfamiView protein in Pfam
PF02867 Ribonuc_red_lgC, 1 hit
PF00317 Ribonuc_red_lgN, 1 hit
PF08343 RNR_N, 1 hit
PRINTSiPR01183 RIBORDTASEM1
SUPFAMiSSF48168 SSF48168, 1 hit
TIGRFAMsiTIGR02506 NrdE_NrdA, 1 hit
TIGR04170 RNR_1b_NrdE, 1 hit
PROSITEiView protein in PROSITE
PS00089 RIBORED_LARGE, 1 hit

Sequencei

Sequence statusi: Complete.

P78027-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKEKIPAF NTQEDLESYI SLNAYTKVYG DFKMDLHAVE AYIQEHVKPK
60 70 80 90 100
TKVFHSTKER LDFLVKNDYY DENIINMYSF EQFEEITRKA YAYRFRYANF
110 120 130 140 150
MGAFKFYNAY ALKTFDGKWY LENYEDRVVM NVLFLANGNY NKALKLLKQI
160 170 180 190 200
ITNRFQPATP TFLNAGRKKR GEFVSCYLLR IEDNMESIGR AITTTLQLSK
210 220 230 240 250
RDGGVALLLT NIRESGAPIK KIENQSSGII PIMKLLEDSF SYANQLGQRQ
260 270 280 290 300
GAGAVYLHAH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFTLAKN
310 320 330 340 350
NEEMALFSPY DVYEEYGKPL SDISVTEMYY ELLANQRIKK TFINARKFFQ
360 370 380 390 400
TVAELHFESG YPYILFDDTV NRRNAHPNRI VMSNLCSEIV QPSTPSEFHH
410 420 430 440 450
DLAFKKVGND ISCNLGSLNI AKAMESGPEF SELVKLAIES LDLVSRVSNL
460 470 480 490 500
ETAPSIQKGN SENHALGLGA MNLHGFLATN QIYYNSPEAI DFTNIFFYTV
510 520 530 540 550
AYHAFKASSE LALEKGKFKN FENTKFADGS YFDKYIKVEP DFWTPKTERV
560 570 580 590 600
KALFQKYQVE IPTRENWKEL ALNIQKNGLA NSHLLAIAPT GSISYLSSCT
610 620 630 640 650
PSLQPVVSPV EVRKEGRLGR IYVPAYQLNK DSYPFYKDGA YELGPEPIIN
660 670 680 690 700
IAAAAQQHVD QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ
710 720
EVLEDSEDHT IQMQQCEACV I
Length:721
Mass (Da):82,375
Last modified:February 1, 1997 - v1
Checksum:i93CAEA95A2A9E647
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA Translation: AAB96160.1
PIRiS73838
RefSeqiNP_110012.1, NC_000912.1
WP_010874680.1, NC_000912.1

Genome annotation databases

EnsemblBacteriaiAAB96160; AAB96160; MPN_324
GeneIDi876946
KEGGimpn:MPN324
PATRICifig|272634.6.peg.348

Similar proteinsi

Entry informationi

Entry nameiRIR1_MYCPN
AccessioniPrimary (citable) accession number: P78027
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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