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P78027

- RIR1_MYCPN

UniProt

P78027 - RIR1_MYCPN

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Protein
Ribonucleoside-diphosphate reductase subunit alpha
Gene
nrdE, MPN_324, MP512
Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei159 – 1591Substrate By similarity
Sitei176 – 1761Important for hydrogen atom transfer By similarity
Sitei183 – 1831Allosteric effector binding By similarity
Binding sitei204 – 2041Substrate; via amide nitrogen By similarity
Sitei213 – 2131Allosteric effector binding By similarity
Active sitei384 – 3841Proton acceptor By similarity
Active sitei386 – 3861Cysteine radical intermediate By similarity
Active sitei388 – 3881Proton acceptor By similarity
Sitei413 – 4131Important for hydrogen atom transfer By similarity
Sitei694 – 6941Important for electron transfer By similarity
Sitei695 – 6951Important for electron transfer By similarity
Sitei716 – 7161Interacts with thioredoxin/glutaredoxin By similarity
Sitei719 – 7191Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-341-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdE
Ordered Locus Names:MPN_324
ORF Names:MP512
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi176 ↔ 413Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP78027.

PTM databases

PhosSiteiP1011996.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

IntActiP78027. 7 interactions.
STRINGi272634.MPN324.

Structurei

3D structure databases

ProteinModelPortaliP78027.
SMRiP78027. Positions 18-700.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 1762Substrate binding By similarity
Regioni384 – 3885Substrate binding By similarity
Regioni589 – 5935Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
KOiK00525.
OMAiIYYIRIR.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78027-1 [UniParc]FASTAAdd to Basket

« Hide

MSVKEKIPAF NTQEDLESYI SLNAYTKVYG DFKMDLHAVE AYIQEHVKPK    50
TKVFHSTKER LDFLVKNDYY DENIINMYSF EQFEEITRKA YAYRFRYANF 100
MGAFKFYNAY ALKTFDGKWY LENYEDRVVM NVLFLANGNY NKALKLLKQI 150
ITNRFQPATP TFLNAGRKKR GEFVSCYLLR IEDNMESIGR AITTTLQLSK 200
RDGGVALLLT NIRESGAPIK KIENQSSGII PIMKLLEDSF SYANQLGQRQ 250
GAGAVYLHAH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFTLAKN 300
NEEMALFSPY DVYEEYGKPL SDISVTEMYY ELLANQRIKK TFINARKFFQ 350
TVAELHFESG YPYILFDDTV NRRNAHPNRI VMSNLCSEIV QPSTPSEFHH 400
DLAFKKVGND ISCNLGSLNI AKAMESGPEF SELVKLAIES LDLVSRVSNL 450
ETAPSIQKGN SENHALGLGA MNLHGFLATN QIYYNSPEAI DFTNIFFYTV 500
AYHAFKASSE LALEKGKFKN FENTKFADGS YFDKYIKVEP DFWTPKTERV 550
KALFQKYQVE IPTRENWKEL ALNIQKNGLA NSHLLAIAPT GSISYLSSCT 600
PSLQPVVSPV EVRKEGRLGR IYVPAYQLNK DSYPFYKDGA YELGPEPIIN 650
IAAAAQQHVD QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ 700
EVLEDSEDHT IQMQQCEACV I 721
Length:721
Mass (Da):82,375
Last modified:February 1, 1997 - v1
Checksum:i93CAEA95A2A9E647
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB96160.1.
PIRiS73838.
RefSeqiNP_110012.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96160; AAB96160; MPN_324.
GeneIDi876946.
KEGGimpn:MPN324.
PATRICi20022002. VBIMycPne110_0348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB96160.1 .
PIRi S73838.
RefSeqi NP_110012.1. NC_000912.1.

3D structure databases

ProteinModelPortali P78027.
SMRi P78027. Positions 18-700.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P78027. 7 interactions.
STRINGi 272634.MPN324.

PTM databases

PhosSitei P1011996.

Proteomic databases

PaxDbi P78027.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB96160 ; AAB96160 ; MPN_324 .
GeneIDi 876946.
KEGGi mpn:MPN324.
PATRICi 20022002. VBIMycPne110_0348.

Phylogenomic databases

eggNOGi COG0209.
KOi K00525.
OMAi IYYIRIR.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci MPNE272634:GJ6Z-341-MONOMER.

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiRIR1_MYCPN
AccessioniPrimary (citable) accession number: P78027
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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