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P78027 (RIR1_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase subunit alpha

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase
Gene names
Name:nrdE
Ordered Locus Names:MPN_324
ORF Names:MP512
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length721 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulation

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase large chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 721721Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187218

Regions

Region175 – 1762Substrate binding By similarity
Region384 – 3885Substrate binding By similarity
Region589 – 5935Substrate binding By similarity

Sites

Active site3841Proton acceptor By similarity
Active site3861Cysteine radical intermediate By similarity
Active site3881Proton acceptor By similarity
Binding site1591Substrate By similarity
Binding site2041Substrate; via amide nitrogen By similarity
Site1761Important for hydrogen atom transfer By similarity
Site1831Allosteric effector binding By similarity
Site2131Allosteric effector binding By similarity
Site4131Important for hydrogen atom transfer By similarity
Site6941Important for electron transfer By similarity
Site6951Important for electron transfer By similarity
Site7161Interacts with thioredoxin/glutaredoxin By similarity
Site7191Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond176 ↔ 413Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
P78027 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 93CAEA95A2A9E647

FASTA72182,375
        10         20         30         40         50         60 
MSVKEKIPAF NTQEDLESYI SLNAYTKVYG DFKMDLHAVE AYIQEHVKPK TKVFHSTKER 

        70         80         90        100        110        120 
LDFLVKNDYY DENIINMYSF EQFEEITRKA YAYRFRYANF MGAFKFYNAY ALKTFDGKWY 

       130        140        150        160        170        180 
LENYEDRVVM NVLFLANGNY NKALKLLKQI ITNRFQPATP TFLNAGRKKR GEFVSCYLLR 

       190        200        210        220        230        240 
IEDNMESIGR AITTTLQLSK RDGGVALLLT NIRESGAPIK KIENQSSGII PIMKLLEDSF 

       250        260        270        280        290        300 
SYANQLGQRQ GAGAVYLHAH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFTLAKN 

       310        320        330        340        350        360 
NEEMALFSPY DVYEEYGKPL SDISVTEMYY ELLANQRIKK TFINARKFFQ TVAELHFESG 

       370        380        390        400        410        420 
YPYILFDDTV NRRNAHPNRI VMSNLCSEIV QPSTPSEFHH DLAFKKVGND ISCNLGSLNI 

       430        440        450        460        470        480 
AKAMESGPEF SELVKLAIES LDLVSRVSNL ETAPSIQKGN SENHALGLGA MNLHGFLATN 

       490        500        510        520        530        540 
QIYYNSPEAI DFTNIFFYTV AYHAFKASSE LALEKGKFKN FENTKFADGS YFDKYIKVEP 

       550        560        570        580        590        600 
DFWTPKTERV KALFQKYQVE IPTRENWKEL ALNIQKNGLA NSHLLAIAPT GSISYLSSCT 

       610        620        630        640        650        660 
PSLQPVVSPV EVRKEGRLGR IYVPAYQLNK DSYPFYKDGA YELGPEPIIN IAAAAQQHVD 

       670        680        690        700        710        720 
QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ EVLEDSEDHT IQMQQCEACV 


I 

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References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96160.1.
PIRS73838.
RefSeqNP_110012.1. NC_000912.1.

3D structure databases

ProteinModelPortalP78027.
SMRP78027. Positions 18-700.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP78027. 7 interactions.
STRING272634.MPN324.

PTM databases

PhosSiteP1011996.

Proteomic databases

PaxDbP78027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96160; AAB96160; MPN_324.
GeneID876946.
KEGGmpn:MPN324.
PATRIC20022002. VBIMycPne110_0348.

Phylogenomic databases

eggNOGCOG0209.
KOK00525.
OMAIHPIVSR.
OrthoDBEOG6J48HC.
ProtClustDBPRK07187.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-341-MONOMER.
UniPathwayUPA00326.

Family and domain databases

InterProIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
SUPFAMSSF48168. SSF48168. 1 hit.
TIGRFAMsTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR1_MYCPN
AccessionPrimary (citable) accession number: P78027
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 13, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names