Reviewed, UniProtKB/Swiss-Prot P78025 (LON_MYCPN)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:	lon
Ordered Locus Names:	MPN_332
ORF Names:	MP504

Organism

Mycoplasma pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

2104 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Degrades the regulatory proteins rcsA and sulA. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 795	795	ATP-dependent protease La		PRO_0000076140
Regions
	Domain	8 – 208	201	Lon
	Nucleotide binding	379 – 386	8	ATP Potential
	Compositional bias	304 – 308	5	Poly-Ser
Sites
	Active site	702	1	By similarity
	Active site	745	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P78025-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7E7855082060C891	FASTA	795	90,204
	10 20 30 40 50 60 MPAVKKPQIL VVRNQVIFPY NGFELDVGRE RSKKLIKALK NLKTKRLVLV TQKNSDQLNP 70 80 90 100 110 120 EFDDIYHCGT LCDIDEIIEV PSEDGKTADY KIKGKGLQRV AITSFSDADL TKYDHHFLNS 130 140 150 160 170 180 TLTENKALDK LLERIFPDKE DFAEILDSLN SFLELQELKK LSKVPKDIKR YDIITFKLAS 190 200 210 220 230 240 LIFKDITLQQ AILEENDIEK RLQKIIGSGI EDLGHISEEA RAKQRESEFD KIDNRITRKV 250 260 270 280 290 300 NEQLSRQQRD FYLREKLRVI REEIGMTSKK EDEVSNIRKK LEENPYPEHI KKRILSELDH 310 320 330 340 350 360 FENSSSSSQE STLTKTYIDT LMNLPWWQES KDNADVKNLI KILNKNHSGL DKVKERVVEY 370 380 390 400 410 420 LAVQLRTKKL KGPIMCLVGP PGVGKSSLAK SIAEALNKCF VKVSLGGVHD ESEIRGHRKT 430 440 450 460 470 480 YLGSMPGRIL KGMVRAKVIN PLFLLDEIDK MTSSNQGYPS GALLEVLDPE LNNKFSDNYV 490 500 510 520 530 540 EEDYDLSKVM FVATANYIED IPEALLDRME VIELTSYTEQ EKLQITKSHL VKRCLDDAEI 550 560 570 580 590 600 KTDDLKFTDE GISYIIKFYT REAGVRQLER LIQQIVRKYI VNLQKTGEQQ VVVDVDLVKK 610 620 630 640 650 660 YLKKEIFDYT VRDEDALPGI VNGMAYTPTG GDLLPIEVTH VAGKGDLILT GNLKQTMRES 670 680 690 700 710 720 ASVALGYVKA NAQSFNINPN LFKKVDINIH VPGGGIPKDG PSAGAALVTA IISSLTGKKV 730 740 750 760 770 780 DPKIAMTGEI TLRGKVMTIG GVKEKTISAY RGGVRTIFMP EKNERYLDEV PKDIVKDLEI 790 ILVKEYKDIY NKIFN

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00089 Genomic DNA. Translation: AAB96152.1.
PIR	S73830.
RefSeq	NP_110020.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S16.004.
Genome annotation databases
GeneID	877195.
GenomeReviews	Gene locus MPN_332 in contig U00089_GR.
KEGG	mpn:MPN332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P78025.
OMA	P78025. HTLISII.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN332-MON.
BRENDA	3.4.21.53. 39500.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_MYCPN

Accession

Primary (citable) accession number: P78025

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)