P78025 (LON_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:	lon
Ordered Locus Names:	MPN_332
ORF Names:	MP504

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm.
Induction	By heat shock By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-2258550,EBI-2258550

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 795	795	Lon protease		PRO_0000076140
Regions
	Domain	9 – 206	198	Lon
	Nucleotide binding	379 – 386	8	ATP By similarity
	Compositional bias	304 – 308	5	Poly-Ser
Sites
	Active site	702	1	By similarity
	Active site	745	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P78025 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7E7855082060C891	FASTA	795	90,204
	10 20 30 40 50 60 MPAVKKPQIL VVRNQVIFPY NGFELDVGRE RSKKLIKALK NLKTKRLVLV TQKNSDQLNP 70 80 90 100 110 120 EFDDIYHCGT LCDIDEIIEV PSEDGKTADY KIKGKGLQRV AITSFSDADL TKYDHHFLNS 130 140 150 160 170 180 TLTENKALDK LLERIFPDKE DFAEILDSLN SFLELQELKK LSKVPKDIKR YDIITFKLAS 190 200 210 220 230 240 LIFKDITLQQ AILEENDIEK RLQKIIGSGI EDLGHISEEA RAKQRESEFD KIDNRITRKV 250 260 270 280 290 300 NEQLSRQQRD FYLREKLRVI REEIGMTSKK EDEVSNIRKK LEENPYPEHI KKRILSELDH 310 320 330 340 350 360 FENSSSSSQE STLTKTYIDT LMNLPWWQES KDNADVKNLI KILNKNHSGL DKVKERVVEY 370 380 390 400 410 420 LAVQLRTKKL KGPIMCLVGP PGVGKSSLAK SIAEALNKCF VKVSLGGVHD ESEIRGHRKT 430 440 450 460 470 480 YLGSMPGRIL KGMVRAKVIN PLFLLDEIDK MTSSNQGYPS GALLEVLDPE LNNKFSDNYV 490 500 510 520 530 540 EEDYDLSKVM FVATANYIED IPEALLDRME VIELTSYTEQ EKLQITKSHL VKRCLDDAEI 550 560 570 580 590 600 KTDDLKFTDE GISYIIKFYT REAGVRQLER LIQQIVRKYI VNLQKTGEQQ VVVDVDLVKK 610 620 630 640 650 660 YLKKEIFDYT VRDEDALPGI VNGMAYTPTG GDLLPIEVTH VAGKGDLILT GNLKQTMRES 670 680 690 700 710 720 ASVALGYVKA NAQSFNINPN LFKKVDINIH VPGGGIPKDG PSAGAALVTA IISSLTGKKV 730 740 750 760 770 780 DPKIAMTGEI TLRGKVMTIG GVKEKTISAY RGGVRTIFMP EKNERYLDEV PKDIVKDLEI 790 ILVKEYKDIY NKIFN

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96152.1.
PIR	S73830.
RefSeq	NP_110020.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P78025.
ModBase	Search...
Protein-protein interaction databases
IntAct	P78025. 1 interaction.
Protein family/group databases
MEROPS	S16.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877195.
GenomeReviews	Gene locus MPN_332 in contig U00089_GR.
KEGG	mpn:MPN332.
PATRIC	20022018. VBIMycPne110_0356.
Phylogenomic databases
HOGENOM	HBG566281.
OMA	TVILQGK.
PhylomeDB	P78025.
ProtClustDB	CLSK494823.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN332-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_MYCPN

Accession

Primary (citable) accession number: P78025

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program