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P78017 (GRPE_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein GrpE
Alternative name(s):
HSP-70 cofactor
Gene names
Name:grpE
Ordered Locus Names:MPN_120
ORF Names:MP034
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length217 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding By similarity. HAMAP-Rule MF_01151

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01151

Subcellular location

Cytoplasm Probable HAMAP-Rule MF_01151.

Sequence similarities

Belongs to the GrpE family.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   Molecular functionChaperone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionadenyl-nucleotide exchange factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 217217Protein GrpE HAMAP-Rule MF_01151
PRO_0000113821

Sequences

Sequence LengthMass (Da)Tools
P78017 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 3C66757AF4BEBEC3

FASTA21724,706
        10         20         30         40         50         60 
MSENSLTITE ILSSIRTLLV KHNKAKVTQI EKELLQAVAE LEKKFKQQVQ NFNELQQKIP 

        70         80         90        100        110        120 
NLQKVNEEFR LKVEKIQEEA QKKIQEKVAE LTIKSKEELE NAKKYVIEKS IDQPLIIIDQ 

       130        140        150        160        170        180 
FEIALSYAQK DPQVKNYTTG FNMVLDAFSR WLEGFGVTKI AIEPGAQFDE KVMAALEVVP 

       190        200        210 
SDQPANTVVK VSKSGYKLHD KVIRFASVVV SQGNKTE 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.
[2]"Towards a two-dimensional proteome map of Mycoplasma pneumoniae."
Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R.
Electrophoresis 21:3765-3780(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95682.1.
PIRS73360.
RefSeqNP_109808.1. NC_000912.1.

3D structure databases

ProteinModelPortalP78017.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP78017. 1 interaction.
STRING272634.MPN120.

Proteomic databases

PaxDbP78017.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95682; AAB95682; MPN_120.
GeneID877237.
KEGGmpn:MPN120.
PATRIC20021539. VBIMycPne110_0127.

Phylogenomic databases

eggNOGCOG0576.
KOK03687.
OMAQPFFEDF.
OrthoDBEOG64BQ6C.
ProtClustDBCLSK378131.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-126-MONOMER.

Family and domain databases

Gene3D2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPMF_01151. GrpE.
InterProIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERPTHR21237. PTHR21237. 1 hit.
PfamPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSPR00773. GRPEPROTEIN.
SUPFAMSSF51064. SSF51064. 1 hit.
PROSITEPS01071. GRPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGRPE_MYCPN
AccessionPrimary (citable) accession number: P78017
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names