P78017 (GRPE_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein GrpE

Alternative name(s):
HSP-70 cofactor

Gene names

Name:	grpE
Ordered Locus Names:	MPN_120
ORF Names:	MP034

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding By similarity. HAMAP-Rule MF_01151
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_01151.
Sequence similarities	Belongs to the GrpE family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Protein GrpE HAMAP-Rule MF_01151		PRO_0000113821

Sequences

	Sequence		Length	Mass (Da)
	P78017 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 3C66757AF4BEBEC3	FASTA	217	24,706
	10 20 30 40 50 60 MSENSLTITE ILSSIRTLLV KHNKAKVTQI EKELLQAVAE LEKKFKQQVQ NFNELQQKIP 70 80 90 100 110 120 NLQKVNEEFR LKVEKIQEEA QKKIQEKVAE LTIKSKEELE NAKKYVIEKS IDQPLIIIDQ 130 140 150 160 170 180 FEIALSYAQK DPQVKNYTTG FNMVLDAFSR WLEGFGVTKI AIEPGAQFDE KVMAALEVVP 190 200 210 SDQPANTVVK VSKSGYKLHD KVIRFASVVV SQGNKTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Towards a two-dimensional proteome map of Mycoplasma pneumoniae." Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R. Electrophoresis 21:3765-3780(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95682.1.
PIR	S73360.
RefSeq	NP_109808.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P78017.
ModBase	Search...
Protein-protein interaction databases
IntAct	P78017. 1 interaction.
STRING	272634.MPN120.
Proteomic databases
PaxDb	P78017.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB95682; AAB95682; MPN_120.
GeneID	877237.
KEGG	mpn:MPN120.
PATRIC	20021539. VBIMycPne110_0127.
Phylogenomic databases
eggNOG	COG0576.
KO	K03687.
OMA	IDQFEIA.
ProtClustDB	CLSK378131.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-126-MONOMER.
Family and domain databases
Gene3D	2.30.22.10. 1 hit. 3.90.20.20. 1 hit.
HAMAP	MF_01151. GrpE.
InterPro	IPR000740. GrpE. IPR013805. GrpE_coiled_coil. IPR009012. GrpE_head. [Graphical view]
PANTHER	PTHR21237. PTHR21237. 1 hit.
Pfam	PF01025. GrpE. 1 hit. [Graphical view]
PRINTS	PR00773. GRPEPROTEIN.
SUPFAM	SSF51064. GrpE_head. 1 hit.
PROSITE	PS01071. GRPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRPE_MYCPN

Accession

Primary (citable) accession number: P78017

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program