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P78011 (GLYA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine hydroxymethyltransferase

Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:MPN_576
ORF Names:MP266
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity. HAMAP-Rule MF_00051

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP-Rule MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1. HAMAP-Rule MF_00051

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00051

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00051.

Sequence similarities

Belongs to the SHMT family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
One-carbon metabolism
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycine biosynthetic process from serine

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglycine hydroxymethyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

pyridoxal phosphate binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406Serine hydroxymethyltransferase HAMAP-Rule MF_00051
PRO_0000113616

Regions

Region115 – 1173Substrate binding By similarity

Sites

Binding site251Pyridoxal phosphate By similarity
Binding site451Pyridoxal phosphate By similarity
Binding site471Substrate By similarity
Binding site541Substrate By similarity
Binding site551Pyridoxal phosphate By similarity
Binding site891Pyridoxal phosphate By similarity
Binding site1111Substrate; via carbonyl oxygen By similarity
Binding site1661Pyridoxal phosphate By similarity
Binding site1941Pyridoxal phosphate By similarity
Binding site2191Pyridoxal phosphate By similarity
Binding site2261Pyridoxal phosphate By similarity
Binding site2521Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3481Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2201N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P78011 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 0460FEA4F730E356

FASTA40645,266
        10         20         30         40         50         60 
MEPKIRRILN KELQRQRDCI CLIASENYVS RDILEVTGSI LTNKYAEGYP TRRFYEGCEV 

        70         80         90        100        110        120 
VDESESLAIN TCKELFGAKW ANVQPHSGSS ANYAVYLALL KPGDAILGLD LNCGGHLTHG 

       130        140        150        160        170        180 
NKFNFSGKQY QPYSYTINPE TEMLDYDEVL RVAREVKPKL IICGFSNYSR TVDFERFSAI 

       190        200        210        220        230        240 
AKEVGAYLLA DIAHIAGLVA AGLHPNPLPY TDVVTSTTHK TLRGPRGGLI MSNNEAIIRK 

       250        260        270        280        290        300 
LDSGVFPGCQ GGPLQHVIAA KYVCFKEALQ PKYKQYIQNV KTNAASMASW FKQQGYRVIS 

       310        320        330        340        350        360 
NGTDTHLFSL DVGKGKDVSQ WLQQANIVLN MNTVPFDKNP AINPSGIRIG TPAMTTRGFK 

       370        380        390        400 
EKHFLYVAAL IDKIIKSDGN KKVIKEVKKA VLKLLERFPL YKGLEY 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95914.1.
PIRS73592.
RefSeqNP_110265.1. NC_000912.1.

3D structure databases

ProteinModelPortalP78011.
SMRP78011. Positions 2-400.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272634.MPN576.

Proteomic databases

PaxDbP78011.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95914; AAB95914; MPN_576.
GeneID876801.
KEGGmpn:MPN576.
PATRIC20022631. VBIMycPne110_0638.

Phylogenomic databases

eggNOGCOG0112.
KOK00600.
OMAMLIDLRN.
OrthoDBEOG6Z0QB2.
ProtClustDBPRK00011.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-581.
MPNE272634:GJ6Z-622-MONOMER.
UniPathwayUPA00193.
UPA00288; UER01023.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00051. SHMT.
InterProIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERPTHR11680. PTHR11680. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_MYCPN
AccessionPrimary (citable) accession number: P78011
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 19, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names