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P78011

- GLYA_MYCPN

UniProt

P78011 - GLYA_MYCPN

Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251Pyridoxal phosphateUniRule annotation
    Binding sitei45 – 451Pyridoxal phosphateUniRule annotation
    Binding sitei47 – 471SubstrateUniRule annotation
    Binding sitei54 – 541SubstrateUniRule annotation
    Binding sitei55 – 551Pyridoxal phosphateUniRule annotation
    Binding sitei89 – 891Pyridoxal phosphateUniRule annotation
    Binding sitei111 – 1111Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei166 – 1661Pyridoxal phosphateUniRule annotation
    Binding sitei194 – 1941Pyridoxal phosphateUniRule annotation
    Binding sitei219 – 2191Pyridoxal phosphateUniRule annotation
    Binding sitei226 – 2261Pyridoxal phosphateUniRule annotation
    Binding sitei252 – 2521Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei348 – 3481Pyridoxal phosphateUniRule annotation

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-581.
    MPNE272634:GJ6Z-622-MONOMER.
    UniPathwayiUPA00193.
    UPA00288; UER01023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
    Short name:
    SHMTUniRule annotation
    Short name:
    Serine methylaseUniRule annotation
    Gene namesi
    Name:glyAUniRule annotation
    Ordered Locus Names:MPN_576
    ORF Names:MP266
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406Serine hydroxymethyltransferasePRO_0000113616Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei220 – 2201N6-(pyridoxal phosphate)lysineUniRule annotation

    Proteomic databases

    PaxDbiP78011.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272634.MPN576.

    Structurei

    3D structure databases

    ProteinModelPortaliP78011.
    SMRiP78011. Positions 2-400.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni115 – 1173Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the SHMT family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0112.
    KOiK00600.
    OMAiHLMLVDV.
    OrthoDBiEOG6Z0QB2.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P78011-1 [UniParc]FASTAAdd to Basket

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    MEPKIRRILN KELQRQRDCI CLIASENYVS RDILEVTGSI LTNKYAEGYP    50
    TRRFYEGCEV VDESESLAIN TCKELFGAKW ANVQPHSGSS ANYAVYLALL 100
    KPGDAILGLD LNCGGHLTHG NKFNFSGKQY QPYSYTINPE TEMLDYDEVL 150
    RVAREVKPKL IICGFSNYSR TVDFERFSAI AKEVGAYLLA DIAHIAGLVA 200
    AGLHPNPLPY TDVVTSTTHK TLRGPRGGLI MSNNEAIIRK LDSGVFPGCQ 250
    GGPLQHVIAA KYVCFKEALQ PKYKQYIQNV KTNAASMASW FKQQGYRVIS 300
    NGTDTHLFSL DVGKGKDVSQ WLQQANIVLN MNTVPFDKNP AINPSGIRIG 350
    TPAMTTRGFK EKHFLYVAAL IDKIIKSDGN KKVIKEVKKA VLKLLERFPL 400
    YKGLEY 406
    Length:406
    Mass (Da):45,266
    Last modified:February 1, 1997 - v1
    Checksum:i0460FEA4F730E356
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB95914.1.
    PIRiS73592.
    RefSeqiNP_110265.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB95914; AAB95914; MPN_576.
    GeneIDi876801.
    KEGGimpn:MPN576.
    PATRICi20022631. VBIMycPne110_0638.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB95914.1 .
    PIRi S73592.
    RefSeqi NP_110265.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P78011.
    SMRi P78011. Positions 2-400.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272634.MPN576.

    Proteomic databases

    PaxDbi P78011.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB95914 ; AAB95914 ; MPN_576 .
    GeneIDi 876801.
    KEGGi mpn:MPN576.
    PATRICi 20022631. VBIMycPne110_0638.

    Phylogenomic databases

    eggNOGi COG0112.
    KOi K00600.
    OMAi HLMLVDV.
    OrthoDBi EOG6Z0QB2.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    UPA00288 ; UER01023 .
    BioCyci MetaCyc:MONOMER-581.
    MPNE272634:GJ6Z-622-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiGLYA_MYCPN
    AccessioniPrimary (citable) accession number: P78011
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3