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P78011 (GLYA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:MPN_576
ORF Names:MP266
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_0000113616

Regions

Region115 – 1173Substrate binding By similarity

Sites

Binding site251Pyridoxal phosphate By similarity
Binding site451Pyridoxal phosphate By similarity
Binding site471Substrate By similarity
Binding site541Substrate binding By similarity
Binding site551Pyridoxal phosphate By similarity
Binding site891Pyridoxal phosphate By similarity
Binding site1111Substrate By similarity
Binding site1661Pyridoxal phosphate By similarity
Binding site1941Pyridoxal phosphate By similarity
Binding site2191Pyridoxal phosphate By similarity
Binding site2261Pyridoxal phosphate By similarity
Binding site2521Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3481Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2201N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P78011 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 0460FEA4F730E356

FASTA40645,266
        10         20         30         40         50         60 
MEPKIRRILN KELQRQRDCI CLIASENYVS RDILEVTGSI LTNKYAEGYP TRRFYEGCEV 

        70         80         90        100        110        120 
VDESESLAIN TCKELFGAKW ANVQPHSGSS ANYAVYLALL KPGDAILGLD LNCGGHLTHG 

       130        140        150        160        170        180 
NKFNFSGKQY QPYSYTINPE TEMLDYDEVL RVAREVKPKL IICGFSNYSR TVDFERFSAI 

       190        200        210        220        230        240 
AKEVGAYLLA DIAHIAGLVA AGLHPNPLPY TDVVTSTTHK TLRGPRGGLI MSNNEAIIRK 

       250        260        270        280        290        300 
LDSGVFPGCQ GGPLQHVIAA KYVCFKEALQ PKYKQYIQNV KTNAASMASW FKQQGYRVIS 

       310        320        330        340        350        360 
NGTDTHLFSL DVGKGKDVSQ WLQQANIVLN MNTVPFDKNP AINPSGIRIG TPAMTTRGFK 

       370        380        390        400 
EKHFLYVAAL IDKIIKSDGN KKVIKEVKKA VLKLLERFPL YKGLEY

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U00089 Genomic DNA. Translation: AAB95914.1.
PIRS73592.
RefSeqNP_110265.1. NC_000912.1.

3D structure databases

ProteinModelPortalP78011.
SMRP78011. Positions 2-400.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID876801.
GenomeReviewsGene locus MPN_576 in contig U00089_GR.
KEGGmpn:MPN576.
PATRIC20022631. VBIMycPne110_0638.

Phylogenomic databases

HOGENOMHBG301263.
OMAADVEANV.
PhylomeDBP78011.
ProtClustDBPRK00011.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-581.
MPNE272634:MPN576-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_MYCPN
AccessionPrimary (citable) accession number: P78011
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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