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P78004

- DNAJ_MYCPN

UniProt

P78004 - DNAJ_MYCPN

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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Binds 2 zinc ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi165 – 1651Zinc 1UniRule annotation
Metal bindingi168 – 1681Zinc 1UniRule annotation
Metal bindingi183 – 1831Zinc 2UniRule annotation
Metal bindingi186 – 1861Zinc 2UniRule annotation
Metal bindingi209 – 2091Zinc 2UniRule annotation
Metal bindingi212 – 2121Zinc 2UniRule annotation
Metal bindingi223 – 2231Zinc 1UniRule annotation
Metal bindingi226 – 2261Zinc 1UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri152 – 23584CR-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein folding Source: InterPro
  3. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-23-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:MPN_021
ORF Names:MP133
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Chaperone protein DnaJPRO_0000070833Add
BLAST

Proteomic databases

PaxDbiP78004.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP78004. 1 interaction.
STRINGi272634.MPN021.

Structurei

3D structure databases

ProteinModelPortaliP78004.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7975JUniRule annotationAdd
BLAST
Repeati165 – 1728CXXCXGXG motif
Repeati183 – 1908CXXCXGXG motif
Repeati209 – 2168CXXCXGXG motif
Repeati223 – 2308CXXCXGXG motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi84 – 13552Gly-richAdd
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation
Contains 1 CR-type zinc finger.UniRule annotation
Contains 1 J domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri152 – 23584CR-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
KOiK03686.
OMAiCHGNGQV.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P78004-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAGKRDYYE VLGVSRSATA QDIKRAFRKL AMQYHPDRHK GEGETVQKQN
60 70 80 90 100
EEKFKEVNEA YEVLSDTEKR GMYDRFGHEG LNASGFHETG FNPFDIFNSV
110 120 130 140 150
FGEGFSFDMD GGSPFDFIFS RGKKSRQNRV VLPYDLEIAV GVDISFFEMT
160 170 180 190 200
NGCTRTIEYT KRVTCSACDG FGAEGGETGM VSCNSCEGNG FILKNQRSIF
210 220 230 240 250
GTVQSQMLCQ SCGGQGKQAK HKCKTCKGSK YKKVPTTKEI QIPAGIYHGD
260 270 280 290 300
ALVDDHGGNE FGGHVGKLVV HVGVLPSKIF KRVDHNVVAN VLVDPMIAIT
310 320 330 340 350
GGKIELPTLE GIKEFNLRAG TKSGEQIVIP GGGIKFTKNF RKKAGDLIII
360 370 380 390
ISYARPSEYS APELRKLKEF IKPNQEVKQY LNALKNEYKS
Length:390
Mass (Da):43,167
Last modified:February 1, 1997 - v1
Checksum:i442E1A6304E8697E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB95781.1.
PIRiS73459.
RefSeqiNP_109709.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95781; AAB95781; MPN_021.
GeneIDi876982.
KEGGimpn:MPN021.
PATRICi20021317. VBIMycPne110_0020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB95781.1 .
PIRi S73459.
RefSeqi NP_109709.1. NC_000912.1.

3D structure databases

ProteinModelPortali P78004.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P78004. 1 interaction.
STRINGi 272634.MPN021.

Proteomic databases

PaxDbi P78004.

Protocols and materials databases

DNASUi 876982.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB95781 ; AAB95781 ; MPN_021 .
GeneIDi 876982.
KEGGi mpn:MPN021.
PATRICi 20021317. VBIMycPne110_0020.

Phylogenomic databases

eggNOGi COG0484.
KOi K03686.
OMAi CHGNGQV.
OrthoDBi EOG6BPDKP.

Enzyme and pathway databases

BioCyci MPNE272634:GJ6Z-23-MONOMER.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPi MF_01152. DnaJ.
InterProi IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiDNAJ_MYCPN
AccessioniPrimary (citable) accession number: P78004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3