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P78004 (DNAJ_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chaperone protein DnaJ
Gene names
Name:dnaJ
Ordered Locus Names:MPN_021
ORF Names:MP133
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity. HAMAP-Rule MF_01152

Cofactor

Binds 2 zinc ions per monomer By similarity. HAMAP-Rule MF_01152

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01152

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01152.

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity. HAMAP-Rule MF_01152

Sequence similarities

Belongs to the DnaJ family.

Contains 1 CR-type zinc finger.

Contains 1 J domain.

Ontologies

Keywords
   Biological processDNA replication
Stress response
   Cellular componentCytoplasm
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionChaperone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

protein folding

Inferred from electronic annotation. Source: InterPro

response to heat

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390Chaperone protein DnaJ HAMAP-Rule MF_01152
PRO_0000070833

Regions

Domain5 – 7975J
Repeat165 – 1728CXXCXGXG motif HAMAP-Rule MF_01152
Repeat183 – 1908CXXCXGXG motif HAMAP-Rule MF_01152
Repeat209 – 2168CXXCXGXG motif HAMAP-Rule MF_01152
Repeat223 – 2308CXXCXGXG motif HAMAP-Rule MF_01152
Zinc finger152 – 23584CR-type HAMAP-Rule MF_01152
Compositional bias84 – 13552Gly-rich HAMAP-Rule MF_01152

Sites

Metal binding1651Zinc 1 By similarity
Metal binding1681Zinc 1 By similarity
Metal binding1831Zinc 2 By similarity
Metal binding1861Zinc 2 By similarity
Metal binding2091Zinc 2 By similarity
Metal binding2121Zinc 2 By similarity
Metal binding2231Zinc 1 By similarity
Metal binding2261Zinc 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P78004 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 442E1A6304E8697E

FASTA39043,167
        10         20         30         40         50         60 
MAAGKRDYYE VLGVSRSATA QDIKRAFRKL AMQYHPDRHK GEGETVQKQN EEKFKEVNEA 

        70         80         90        100        110        120 
YEVLSDTEKR GMYDRFGHEG LNASGFHETG FNPFDIFNSV FGEGFSFDMD GGSPFDFIFS 

       130        140        150        160        170        180 
RGKKSRQNRV VLPYDLEIAV GVDISFFEMT NGCTRTIEYT KRVTCSACDG FGAEGGETGM 

       190        200        210        220        230        240 
VSCNSCEGNG FILKNQRSIF GTVQSQMLCQ SCGGQGKQAK HKCKTCKGSK YKKVPTTKEI 

       250        260        270        280        290        300 
QIPAGIYHGD ALVDDHGGNE FGGHVGKLVV HVGVLPSKIF KRVDHNVVAN VLVDPMIAIT 

       310        320        330        340        350        360 
GGKIELPTLE GIKEFNLRAG TKSGEQIVIP GGGIKFTKNF RKKAGDLIII ISYARPSEYS 

       370        380        390 
APELRKLKEF IKPNQEVKQY LNALKNEYKS 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95781.1.
PIRS73459.
RefSeqNP_109709.1. NC_000912.1.

3D structure databases

ProteinModelPortalP78004.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP78004. 1 interaction.
STRING272634.MPN021.

Proteomic databases

PaxDbP78004.

Protocols and materials databases

DNASU876982.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95781; AAB95781; MPN_021.
GeneID876982.
KEGGmpn:MPN021.
PATRIC20021317. VBIMycPne110_0020.

Phylogenomic databases

eggNOGCOG0484.
KOK03686.
OMACHGNGQV.
OrthoDBEOG6BPDKP.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-23-MONOMER.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPMF_01152. DnaJ.
InterProIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAJ_MYCPN
AccessionPrimary (citable) accession number: P78004
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names