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Reviewed, UniProtKB/Swiss-Prot P78004 (DNAJ_MYCPN)

Last modified November 24, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Chaperone protein dnaJ
Gene names
Name: dnaJ
Ordered Locus Names: MPN_021
ORF Names: MP133
OrganismMycoplasma pneumoniae [Complete proteome] [HAMAP]
Taxonomic identifier2104 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

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Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon interaction with the dnaJ-bound protein, dnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from dnaK; ATP binding to dnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between dnaJ, dnaK and grpE are required for fully efficient folding. Also involved, together with dnaK and grpE, in the DNA replication of plasmids through activation of initiation proteins By similarity.

Cofactor

Binds 2 zinc ions per monomer By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Probable.

Domain

The J domain is necessary and sufficient to stimulate dnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, dnaK-independent chaperone activity of dnaJ. Zinc center 2 is essential for interaction with dnaK and for dnaJ activity By similarity.

Sequence similarities

Belongs to the dnaJ family.

Contains 1 CR-type zinc finger.

Contains 1 J domain.

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Ontologies

Keywords
   Biological processDNA replication
Stress response
   Cellular componentCytoplasm
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionChaperone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

protein folding

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionheat shock protein binding

Inferred from electronic annotation. Source: InterPro

unfolded protein binding

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390Chaperone protein dnaJ HAMAP MF_01152
PRO_0000070833

Regions

Domain5 – 7975J
Repeat165 – 1728CXXCXGXG motif HAMAP MF_01152
Repeat183 – 1908CXXCXGXG motif HAMAP MF_01152
Repeat209 – 2168CXXCXGXG motif HAMAP MF_01152
Repeat223 – 2308CXXCXGXG motif HAMAP MF_01152
Zinc finger152 – 23584CR-type HAMAP MF_01152
Compositional bias84 – 13552Gly-rich HAMAP MF_01152

Sites

Metal binding1651Zinc 1 By similarity
Metal binding1681Zinc 1 By similarity
Metal binding1831Zinc 2 By similarity
Metal binding1861Zinc 2 By similarity
Metal binding2091Zinc 2 By similarity
Metal binding2121Zinc 2 By similarity
Metal binding2231Zinc 1 By similarity
Metal binding2261Zinc 1 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P78004-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 442E1A6304E8697E

FASTA39043,167
        10         20         30         40         50         60 
MAAGKRDYYE VLGVSRSATA QDIKRAFRKL AMQYHPDRHK GEGETVQKQN EEKFKEVNEA 

        70         80         90        100        110        120 
YEVLSDTEKR GMYDRFGHEG LNASGFHETG FNPFDIFNSV FGEGFSFDMD GGSPFDFIFS 

       130        140        150        160        170        180 
RGKKSRQNRV VLPYDLEIAV GVDISFFEMT NGCTRTIEYT KRVTCSACDG FGAEGGETGM 

       190        200        210        220        230        240 
VSCNSCEGNG FILKNQRSIF GTVQSQMLCQ SCGGQGKQAK HKCKTCKGSK YKKVPTTKEI 

       250        260        270        280        290        300 
QIPAGIYHGD ALVDDHGGNE FGGHVGKLVV HVGVLPSKIF KRVDHNVVAN VLVDPMIAIT 

       310        320        330        340        350        360 
GGKIELPTLE GIKEFNLRAG TKSGEQIVIP GGGIKFTKNF RKKAGDLIII ISYARPSEYS 

       370        380        390 
APELRKLKEF IKPNQEVKQY LNALKNEYKS

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References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

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Cross-references

Sequence databases

U00089 Genomic DNA. Translation: AAB95781.1.
PIRS73459.
RefSeqNP_109709.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID876982.
GenomeReviewsGene locus MPN_021 in contig U00089_GR.
KEGGmpn:MPN021.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP78004.
OMAFEHGGMG

Enzyme and pathway databases

BioCycMPNE272634:MPN021-MON.

Family and domain databases

HAMAPMF_01152.
[Tree]
InterProIPR002939. DnaJ_C.
IPR001623. DnaJ_N.
IPR018253. Heat_shock_DnaJ_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR015609. Hsp40/DnaJ_Rel.
IPR003095. Hsp_DnaJ.
IPR001305. HSP_DnaJ_cys-rich.
[Graphical view]
Gene3DG3DSA:1.10.287.110. DnaJ_N. 1 hit.
G3DSA:2.10.230.10. HSP_DnaJ_cys-rich. 1 hit.
PANTHERPTHR11821. Hsp40/DnaJ_Rel. 1 hit.
PfamPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSPR00625. DNAJPROTEIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDNAJ_MYCPN
AccessionPrimary (citable) accession number: P78004
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents