Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P78004

- DNAJ_MYCPN

UniProt

P78004 - DNAJ_MYCPN

Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

    Cofactori

    Binds 2 zinc ions per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi165 – 1651Zinc 1UniRule annotation
    Metal bindingi168 – 1681Zinc 1UniRule annotation
    Metal bindingi183 – 1831Zinc 2UniRule annotation
    Metal bindingi186 – 1861Zinc 2UniRule annotation
    Metal bindingi209 – 2091Zinc 2UniRule annotation
    Metal bindingi212 – 2121Zinc 2UniRule annotation
    Metal bindingi223 – 2231Zinc 1UniRule annotation
    Metal bindingi226 – 2261Zinc 1UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri152 – 23584CR-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein folding Source: InterPro
    3. response to heat Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    DNA replication, Stress response

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMPNE272634:GJ6Z-23-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chaperone protein DnaJUniRule annotation
    Gene namesi
    Name:dnaJUniRule annotation
    Ordered Locus Names:MPN_021
    ORF Names:MP133
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Chaperone protein DnaJPRO_0000070833Add
    BLAST

    Proteomic databases

    PaxDbiP78004.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    IntActiP78004. 1 interaction.
    STRINGi272634.MPN021.

    Structurei

    3D structure databases

    ProteinModelPortaliP78004.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 7975JUniRule annotationAdd
    BLAST
    Repeati165 – 1728CXXCXGXG motif
    Repeati183 – 1908CXXCXGXG motif
    Repeati209 – 2168CXXCXGXG motif
    Repeati223 – 2308CXXCXGXG motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi84 – 13552Gly-richAdd
    BLAST

    Domaini

    The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaJ family.UniRule annotation
    Contains 1 CR-type zinc finger.UniRule annotation
    Contains 1 J domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri152 – 23584CR-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0484.
    KOiK03686.
    OMAiCHGNGQV.
    OrthoDBiEOG6BPDKP.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPiMF_01152. DnaJ.
    InterProiIPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view]
    PfamiPF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P78004-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAGKRDYYE VLGVSRSATA QDIKRAFRKL AMQYHPDRHK GEGETVQKQN    50
    EEKFKEVNEA YEVLSDTEKR GMYDRFGHEG LNASGFHETG FNPFDIFNSV 100
    FGEGFSFDMD GGSPFDFIFS RGKKSRQNRV VLPYDLEIAV GVDISFFEMT 150
    NGCTRTIEYT KRVTCSACDG FGAEGGETGM VSCNSCEGNG FILKNQRSIF 200
    GTVQSQMLCQ SCGGQGKQAK HKCKTCKGSK YKKVPTTKEI QIPAGIYHGD 250
    ALVDDHGGNE FGGHVGKLVV HVGVLPSKIF KRVDHNVVAN VLVDPMIAIT 300
    GGKIELPTLE GIKEFNLRAG TKSGEQIVIP GGGIKFTKNF RKKAGDLIII 350
    ISYARPSEYS APELRKLKEF IKPNQEVKQY LNALKNEYKS 390
    Length:390
    Mass (Da):43,167
    Last modified:February 1, 1997 - v1
    Checksum:i442E1A6304E8697E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB95781.1.
    PIRiS73459.
    RefSeqiNP_109709.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB95781; AAB95781; MPN_021.
    GeneIDi876982.
    KEGGimpn:MPN021.
    PATRICi20021317. VBIMycPne110_0020.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB95781.1 .
    PIRi S73459.
    RefSeqi NP_109709.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P78004.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P78004. 1 interaction.
    STRINGi 272634.MPN021.

    Proteomic databases

    PaxDbi P78004.

    Protocols and materials databases

    DNASUi 876982.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB95781 ; AAB95781 ; MPN_021 .
    GeneIDi 876982.
    KEGGi mpn:MPN021.
    PATRICi 20021317. VBIMycPne110_0020.

    Phylogenomic databases

    eggNOGi COG0484.
    KOi K03686.
    OMAi CHGNGQV.
    OrthoDBi EOG6BPDKP.

    Enzyme and pathway databases

    BioCyci MPNE272634:GJ6Z-23-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPi MF_01152. DnaJ.
    InterProi IPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view ]
    Pfami PF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiDNAJ_MYCPN
    AccessioniPrimary (citable) accession number: P78004
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3