Reviewed, UniProtKB/Swiss-Prot P78003 (METK_MYCPN)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase
    AdoMet synthetase
    MAT

Gene names

Name:	metK
Synonyms:	metX
Ordered Locus Names:	MPN_060
ORF Names:	MP094

Organism

Mycoplasma pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

2104 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP MF_00086
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP MF_00086
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP methionine adenosyltransferase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	S-adenosylmethionine synthetase HAMAP MF_00086		PRO_0000174553
Regions
	Nucleotide binding	256 – 263	8	ATP Potential
Sites
	Metal binding	24	1	Magnesium By similarity
	Metal binding	50	1	Potassium By similarity
	Metal binding	260	1	Potassium By similarity
	Metal binding	268	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P78003-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 6E5817BAF4E65051	FASTA	383	42,560
	10 20 30 40 50 60 MAKTIKHPRW GRYVAEAVGR GHPDKICDQI ADSILDECIK QSPTSHVACE VFASKNLIMV 70 80 90 100 110 120 GGEILTTGYV DVVQTGWKVL NRLGYTENDF SFLSCINSQS SEINQAVQSN DEIGAGDQGI 130 140 150 160 170 180 TVGYACSETE QLMPLGSIVA QALVQRAARI IDQYPFIKHD MKSQVVLNYT GNKVQCESVL 190 200 210 220 230 240 MSVQHTQDVS LDQLRQTIIN QVILPVLTEY GLNDPKIKHL VNPGGSFVVG GPMADTGLTG 250 260 270 280 290 300 RKIIVDTYGP YANHGGGSFS GKDPTKVDRT GAYFARFIAK HIVSLGWAEE CEVSISWVFS 310 320 330 340 350 360 QPLPQSIQVK CFNINKEFSE QLINQVISQY FNWSVAKIIA KLKLLDQVEY FRYAVYGHFG 370 380 HQTAPWEQLS ERDSLQCLIK NFQ

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00089 Genomic DNA. Translation: AAB95742.1.
PIR	S73420.
RefSeq	NP_109748.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	876974.
GenomeReviews	Gene locus MPN_060 in contig U00089_GR.
KEGG	mpn:MPN060.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P78003.
OMA	VPRKLKY
Enzyme and pathway databases
BioCyc	MPNE272634:MPN060-MON.
BRENDA	2.5.1.6. 39500.
Family and domain databases
HAMAP	MF_00086. [Tree]
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK_MYCPN

Accession

Primary (citable) accession number: P78003

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)