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Protein

Beta-galactosidase

Gene

lacZ

Organism
Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei388Proton donorBy similarity1
Active sitei453NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Synonyms:lacA
Ordered Locus Names:Teth39_0611
OrganismiThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Taxonomic identifieri340099 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
Proteomesi
  • UP000002156 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000576771 – 743Beta-galactosidaseAdd BLAST743

Interactioni

Protein-protein interaction databases

STRINGi340099.Teth39_0611.

Structurei

3D structure databases

ProteinModelPortaliP77989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000253353.
KOiK01190.
OMAiKFAAYAY.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
2.60.40.920. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR003344. Big_1_dom.
IPR032311. DUF4982.
IPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR008964. Invasin/intimin_cell_adhesion.
[Graphical view]
PfamiPF16355. DUF4982. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49373. SSF49373. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRDVLNFNV DWLYIPEDLN DAYKFDFDES NFEVVSLPHA NKTFPHHYFK
60 70 80 90 100
EEDYRFVSWY RKHFKVDERY KGKKVYIHFE GVITVAKVYV NGEFVGEHKG
110 120 130 140 150
GYTPFEFDIT EYIKYGNFEN LIAVQVDSRE HKDIPPEGHL VDYMLFGGIY
160 170 180 190 200
RNVWLKILND THIKDVYFVV DKLQDSVAEI SITTTIAGKE ISNGKILTEV
210 220 230 240 250
INKEGVVCSS VVTDIKEMQK EIVQQIKMDN PLTWHPDHPY LYNVSVKLIA
260 270 280 290 300
ENEILDNYTF KTGIRTVEFR DDGKFYINGE PLKLRGLNRH QTFPYVGGAM
310 320 330 340 350
PDRVQRKDAD ILKYELGLNY VRTSHYPQAV SFLDRCDEIG LLVFEEIPGW
360 370 380 390 400
QHIGDENWKN IAKENLKEMI LRDRNHPCIF MWGVRINESL DDHDFYKEMN
410 420 430 440 450
EIAHKLDRSR PTGGVRYLRD SEKLEDVFTY NDFIYNLEGK IQLPNHKKYM
460 470 480 490 500
VTEYMGHMYP TKSYDNLNRL ITHARLHALI QDKQYGIPNM AGASGWCAFD
510 520 530 540 550
YNTTSAFGSG DNICYHGVCD IFRLPKFAAH FYRSQADPHL YGPYVFIASY
560 570 580 590 600
LIPSFEEENG DKLLVFSNCE EVELYINDKF VKRQMPNRVD FPSLPHPPFE
610 620 630 640 650
FSMKECGINY MEVRVNNASI TAIGLIDGKE VARHTLRPYG KPHKLILSCD
660 670 680 690 700
DNEIMADGAD CTRVVVSVVD ENGSILPYAN IPVSFEIEGE GKLIGENPLT
710 720 730 740
LEAGRGAVYV KSTRKPGEII LKAKSHYVAE ESNVSIKTKS IGY
Length:743
Mass (Da):85,765
Last modified:May 20, 2008 - v2
Checksum:iA803F29C65B73A5A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54Y → H in CAA69850 (Ref. 1) Curated1
Sequence conflicti84 – 87TVAK → LLR in CAA69850 (Ref. 1) Curated4
Sequence conflicti265R → KG in CAA69850 (Ref. 1) Curated1
Sequence conflicti341L → M in CAA69850 (Ref. 1) Curated1
Sequence conflicti389S → R in CAA69850 (Ref. 1) Curated1
Sequence conflicti609N → S in CAA69850 (Ref. 1) Curated1
Sequence conflicti618A → S in CAA69850 (Ref. 1) Curated1
Sequence conflicti623I → V in CAA69850 (Ref. 1) Curated1
Sequence conflicti648 – 649SC → TA in CAA69850 (Ref. 1) Curated2
Sequence conflicti652N → V in CAA69850 (Ref. 1) Curated1
Sequence conflicti659A → S in CAA69850 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08557 Genomic DNA. Translation: CAA69850.1.
CP000924 Genomic DNA. Translation: ABY94275.1.
RefSeqiWP_012269093.1. NC_010321.1.

Genome annotation databases

EnsemblBacteriaiABY94275; ABY94275; Teth39_0611.
KEGGitpd:Teth39_0611.
PATRICi23885832. VBIThePse6203_0640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08557 Genomic DNA. Translation: CAA69850.1.
CP000924 Genomic DNA. Translation: ABY94275.1.
RefSeqiWP_012269093.1. NC_010321.1.

3D structure databases

ProteinModelPortaliP77989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi340099.Teth39_0611.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY94275; ABY94275; Teth39_0611.
KEGGitpd:Teth39_0611.
PATRICi23885832. VBIThePse6203_0640.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000253353.
KOiK01190.
OMAiKFAAYAY.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
2.60.40.920. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR003344. Big_1_dom.
IPR032311. DUF4982.
IPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR008964. Invasin/intimin_cell_adhesion.
[Graphical view]
PfamiPF16355. DUF4982. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49373. SSF49373. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_THEP3
AccessioniPrimary (citable) accession number: P77989
Secondary accession number(s): B0K7M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 20, 2008
Last modified: November 2, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.