P77983 (KPYK1_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase I
EC=2.7.1.40

Alternative name(s):
PK-1

Gene names

Name:	pykF
Ordered Locus Names:	STM1378

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Enzyme regulation	Belongs to type I PK; fructose 1,6-bisphosphate-activated.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Pyruvate kinase I		PRO_0000112072
Sites
	Metal binding	34	1	Potassium By similarity
	Metal binding	36	1	Potassium By similarity
	Metal binding	66	1	Potassium By similarity
	Metal binding	67	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	222	1	Magnesium By similarity
	Metal binding	246	1	Magnesium By similarity
	Binding site	32	1	Substrate By similarity
	Binding site	245	1	Substrate; via amide nitrogen By similarity
	Binding site	246	1	Substrate; via amide nitrogen By similarity
	Binding site	278	1	Substrate By similarity
	Site	220	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	142	1	V → C in CAA68205. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P77983 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 116411A30C49AAC0	FASTA	470	50,657
	10 20 30 40 50 60 MKKTKIVCTI GPKTESEEML SKMLDAGMNV MRLNFSHGDY AEHGQRIQNL RNVMSKTGKK 70 80 90 100 110 120 AAILLDTKGP EIRTIKLEGG NDVSLKAGQT FTFTTDKSVV GNNEIVAVTY EGFTSDLSVG 130 140 150 160 170 180 NTVLVDDGLI GMEVTAIEGN KVICKVLNNG DLGENKGVNL PGVSIALPAL AEKDKQDLIF 190 200 210 220 230 240 GCEQGVDFVA ASFIRKRSDV VEIREHLKAH GGENIQIISK IENQEGLNNF DEILEASDGI 250 260 270 280 290 300 MVARGDLGVE IPVEEVIFAQ KMMIEKCIRA RKVVITATQM LDSMIKNPRP TRAEAGDVAN 310 320 330 340 350 360 AILDGTDAVM LSGESAKGKY PLEAVSIMAT ICERTDRVMN SRLDYNNDSR KLRITEAVCR 370 380 390 400 410 420 GAVETAEKLE APLIVVATQG GKSARAVRKY FPDATILALT TNEVTARQLV LSKGVVSQLV 430 440 450 460 470 KEINSTDDFY RLGKDVALQS GLAQKGDVVV MVSGALVPSG TTNTASVHVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the boundaries of Salmonella pathogenicity island 2 and the corresponding chromosomal region of Escherichia coli K-12." Hensel M., Shea J.E., Baeumler A.J., Gleeson C., Blattner F.R., Holden D.W. J. Bacteriol. 179:1105-1111(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99945 Genomic DNA. Translation: CAA68205.1. AE006468 Genomic DNA. Translation: AAL20302.1.
RefSeq	NP_460343.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P77983.
SMR	P77983. Positions 1-470.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM1378.
Proteomic databases
PaxDb	P77983.
PRIDE	P77983.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL20302; AAL20302; STM1378.
GeneID	1252896.
KEGG	stm:STM1378.
PATRIC	32381237. VBISalEnt20916_1461.
Phylogenomic databases
eggNOG	COG0469.
HOGENOM	HOG000021559.
KO	K00873.
OMA	VKMMATI.
ProtClustDB	PRK09206.
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK1_SALTY

Accession

Primary (citable) accession number: P77983

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program