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P77983 (KPYK1_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase I

EC=2.7.1.40
Alternative name(s):
PK-1
Gene names
Name:pykF
Ordered Locus Names:STM1378
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Enzyme regulation

Belongs to type I PK; fructose 1,6-bisphosphate-activated.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 470470Pyruvate kinase I
PRO_0000112072

Sites

Metal binding341Potassium By similarity
Metal binding361Potassium By similarity
Metal binding661Potassium By similarity
Metal binding671Potassium; via carbonyl oxygen By similarity
Metal binding2221Magnesium By similarity
Metal binding2461Magnesium By similarity
Binding site321Substrate By similarity
Binding site2451Substrate; via amide nitrogen By similarity
Binding site2461Substrate; via amide nitrogen By similarity
Binding site2781Substrate By similarity
Site2201Transition state stabilizer By similarity

Experimental info

Sequence conflict1421V → C in CAA68205. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P77983 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 116411A30C49AAC0

FASTA47050,657
        10         20         30         40         50         60 
MKKTKIVCTI GPKTESEEML SKMLDAGMNV MRLNFSHGDY AEHGQRIQNL RNVMSKTGKK 

        70         80         90        100        110        120 
AAILLDTKGP EIRTIKLEGG NDVSLKAGQT FTFTTDKSVV GNNEIVAVTY EGFTSDLSVG 

       130        140        150        160        170        180 
NTVLVDDGLI GMEVTAIEGN KVICKVLNNG DLGENKGVNL PGVSIALPAL AEKDKQDLIF 

       190        200        210        220        230        240 
GCEQGVDFVA ASFIRKRSDV VEIREHLKAH GGENIQIISK IENQEGLNNF DEILEASDGI 

       250        260        270        280        290        300 
MVARGDLGVE IPVEEVIFAQ KMMIEKCIRA RKVVITATQM LDSMIKNPRP TRAEAGDVAN 

       310        320        330        340        350        360 
AILDGTDAVM LSGESAKGKY PLEAVSIMAT ICERTDRVMN SRLDYNNDSR KLRITEAVCR 

       370        380        390        400        410        420 
GAVETAEKLE APLIVVATQG GKSARAVRKY FPDATILALT TNEVTARQLV LSKGVVSQLV 

       430        440        450        460        470 
KEINSTDDFY RLGKDVALQS GLAQKGDVVV MVSGALVPSG TTNTASVHVL 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the boundaries of Salmonella pathogenicity island 2 and the corresponding chromosomal region of Escherichia coli K-12."
Hensel M., Shea J.E., Baeumler A.J., Gleeson C., Blattner F.R., Holden D.W.
J. Bacteriol. 179:1105-1111(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT2.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99945 Genomic DNA. Translation: CAA68205.1.
AE006468 Genomic DNA. Translation: AAL20302.1.
RefSeqNP_460343.1. NC_003197.1.

3D structure databases

ProteinModelPortalP77983.
SMRP77983. Positions 1-470.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM1378.

Proteomic databases

PaxDbP77983.
PRIDEP77983.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL20302; AAL20302; STM1378.
GeneID1252896.
KEGGstm:STM1378.
PATRIC32381237. VBISalEnt20916_1461.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
KOK00873.
OMAHGTRIAN.
OrthoDBEOG6GBMB0.
ProtClustDBPRK09206.

Enzyme and pathway databases

BioCycSENT99287:GCTI-1388-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK1_SALTY
AccessionPrimary (citable) accession number: P77983
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: November 13, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways