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Protein

Argininosuccinate synthase

Gene

argG

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei89 – 891CitrullineUniRule annotation
Binding sitei119 – 1191ATP; via amide nitrogenUniRule annotation
Binding sitei121 – 1211AspartateUniRule annotation
Binding sitei125 – 1251AspartateUniRule annotation
Binding sitei125 – 1251CitrullineUniRule annotation
Binding sitei126 – 1261AspartateUniRule annotation
Binding sitei129 – 1291CitrullineUniRule annotation
Binding sitei177 – 1771CitrullineUniRule annotation
Binding sitei186 – 1861CitrullineUniRule annotation
Binding sitei262 – 2621CitrullineUniRule annotation
Binding sitei274 – 2741CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPUniRule annotation

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: GO_Central
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: GO_Central
  2. argininosuccinate metabolic process Source: GO_Central
  3. urea cycle Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:slr0585
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Argininosuccinate synthasePRO_0000148655Add
BLAST

Proteomic databases

PaxDbiP77973.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
trxAP522312EBI-862317,EBI-862916

Protein-protein interaction databases

IntActiP77973. 7 interactions.
STRINGi1148.slr0585.

Structurei

3D structure databases

ProteinModelPortaliP77973.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiP77973.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.
PhylomeDBiP77973.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77973-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRAKKVVLA YSGGVDTSVC IPYLMHEWGV EEVITLAADL GQGDELGPIQ
60 70 80 90 100
EKALRCGAVE SLVIDGKEEF VKEYAFRSIQ ANALYENRYP LSTALARPLI
110 120 130 140 150
AKMLVEAAEK YGADAVAHGC TGKGNDQVRF DISIMALNPN LKVLAPAREW
160 170 180 190 200
KMSREETIAY GERYGVESPV KKSSPYSIDR NILGRSIEAG PLEDPMTEPT
210 220 230 240 250
EEIYLMTKAI ADTPDEPEYV DIGFEKGIPV SLNGVMLDPV TLVERLNEIA
260 270 280 290 300
GNHGVGRLDM VENRVVGIKS REIYEAPALL VLIDAHRDLE SLTQTADVTH
310 320 330 340 350
YKNTVEEIYS QLIYRGLWYS PLKEALDAFI VKTQERVTGM VRVKFFKGNA
360 370 380 390 400
NVAGRKSDYS IYDAELATYG MEDQFDHKAA EGFIYIWGLP TKVWAQKMRG
Length:400
Mass (Da):44,485
Last modified:February 1, 1997 - v1
Checksum:i37BDB758CBB82C05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18841.1.
PIRiS76929.
RefSeqiNP_443029.1. NC_000911.1.
YP_005653088.1. NC_017277.1.
YP_007452904.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA18841; BAA18841; BAA18841.
GeneIDi951914.
KEGGisyn:slr0585.
PATRICi23843914. VBISynSp132158_3487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18841.1.
PIRiS76929.
RefSeqiNP_443029.1. NC_000911.1.
YP_005653088.1. NC_017277.1.
YP_007452904.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP77973.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP77973. 7 interactions.
STRINGi1148.slr0585.

Proteomic databases

PaxDbiP77973.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18841; BAA18841; BAA18841.
GeneIDi951914.
KEGGisyn:slr0585.
PATRICi23843914. VBISynSp132158_3487.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiP77973.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.
PhylomeDBiP77973.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiASSY_SYNY3
AccessioniPrimary (citable) accession number: P77973
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.