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Protein

Argininosuccinate synthase

Gene

argG

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei89CitrullineUniRule annotation1
Binding sitei119ATP; via amide nitrogenUniRule annotation1
Binding sitei121AspartateUniRule annotation1
Binding sitei125AspartateUniRule annotation1
Binding sitei125CitrullineUniRule annotation1
Binding sitei126AspartateUniRule annotation1
Binding sitei129CitrullineUniRule annotation1
Binding sitei177CitrullineUniRule annotation1
Binding sitei186CitrullineUniRule annotation1
Binding sitei262CitrullineUniRule annotation1
Binding sitei274CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:slr0585
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001486551 – 400Argininosuccinate synthaseAdd BLAST400

Proteomic databases

PRIDEiP77973.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
trxAP522312EBI-862317,EBI-862916

Protein-protein interaction databases

IntActiP77973. 7 interactors.

Structurei

3D structure databases

ProteinModelPortaliP77973.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230093.
InParanoidiP77973.
KOiK01940.
OMAiQCEVVTF.
PhylomeDBiP77973.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77973-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRAKKVVLA YSGGVDTSVC IPYLMHEWGV EEVITLAADL GQGDELGPIQ
60 70 80 90 100
EKALRCGAVE SLVIDGKEEF VKEYAFRSIQ ANALYENRYP LSTALARPLI
110 120 130 140 150
AKMLVEAAEK YGADAVAHGC TGKGNDQVRF DISIMALNPN LKVLAPAREW
160 170 180 190 200
KMSREETIAY GERYGVESPV KKSSPYSIDR NILGRSIEAG PLEDPMTEPT
210 220 230 240 250
EEIYLMTKAI ADTPDEPEYV DIGFEKGIPV SLNGVMLDPV TLVERLNEIA
260 270 280 290 300
GNHGVGRLDM VENRVVGIKS REIYEAPALL VLIDAHRDLE SLTQTADVTH
310 320 330 340 350
YKNTVEEIYS QLIYRGLWYS PLKEALDAFI VKTQERVTGM VRVKFFKGNA
360 370 380 390 400
NVAGRKSDYS IYDAELATYG MEDQFDHKAA EGFIYIWGLP TKVWAQKMRG
Length:400
Mass (Da):44,485
Last modified:February 1, 1997 - v1
Checksum:i37BDB758CBB82C05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18841.1.
PIRiS76929.

Genome annotation databases

EnsemblBacteriaiBAA18841; BAA18841; BAA18841.
KEGGisyn:slr0585.
PATRICi23843914. VBISynSp132158_3487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18841.1.
PIRiS76929.

3D structure databases

ProteinModelPortaliP77973.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP77973. 7 interactors.

Proteomic databases

PRIDEiP77973.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18841; BAA18841; BAA18841.
KEGGisyn:slr0585.
PATRICi23843914. VBISynSp132158_3487.

Phylogenomic databases

HOGENOMiHOG000230093.
InParanoidiP77973.
KOiK01940.
OMAiQCEVVTF.
PhylomeDBiP77973.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_SYNY3
AccessioniPrimary (citable) accession number: P77973
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.