Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P77969

- HEM2_SYNY3

UniProt

P77969 - HEM2_SYNY3

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 1 zinc ion per monomer.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi119 – 1191Zinc; catalyticBy similarity
    Metal bindingi121 – 1211Zinc; catalyticBy similarity
    Metal bindingi129 – 1291Zinc; catalyticBy similarity
    Active sitei198 – 1981Schiff-base intermediate with substrateBy similarity
    Binding sitei208 – 2081Substrate 1By similarity
    Binding sitei220 – 2201Substrate 1By similarity
    Metal bindingi236 – 2361MagnesiumBy similarity
    Active sitei251 – 2511Schiff-base intermediate with substrateBy similarity
    Binding sitei277 – 2771Substrate 2By similarity
    Binding sitei316 – 3161Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:sll1994
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Delta-aminolevulinic acid dehydratasePRO_0000140521Add
    BLAST

    Proteomic databases

    PaxDbiP77969.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    trxAP522311EBI-862729,EBI-862916

    Protein-protein interaction databases

    IntActiP77969. 2 interactions.
    STRINGi1148.sll1994.

    Structurei

    3D structure databases

    ProteinModelPortaliP77969.
    SMRiP77969. Positions 5-326.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    KOiK01698.
    OMAiQINPANS.
    OrthoDBiEOG6VXFCB.
    PhylomeDBiP77969.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P77969-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPTIRPRRL RQTDVLRRMV RENTLTVNDL IYPLFAVPGN AIAKEVVSMP    50
    GVYQLSVDKI VDEAKEVRDL GIPAIILFGI PEDKDTDATG AWHDCGIVQK 100
    ATEAVKKAVP DLVVIVDTCL CEYTNHGHCG YLETGDLTGR VLNDPTLELL 150
    KKTAVSQANA GADVIAPSGM MDGFVQAIRE ALDDHDFQNI PILSYAAKYA 200
    SAYYGPFRDA ADSSPQFGDR RTYQMDPGNS REALKEVELD LLEGADMVMV 250
    KPALSYMDII WRIKEMTNLP VAAYNVSGEY SMVKAAALNG WIDEQKVTLE 300
    TLTSFKRAGA DLILTYHAKD AARWLQD 327
    Length:327
    Mass (Da):36,163
    Last modified:February 1, 1997 - v1
    Checksum:iA7FAA7D5915300C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18067.1.
    PIRiS75506.
    RefSeqiNP_441387.1. NC_000911.1.
    YP_005651444.1. NC_017277.1.
    YP_007451269.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA18067; BAA18067; BAA18067.
    GeneIDi954742.
    KEGGisyn:sll1994.
    syy:SYNGTS_1491.
    syz:MYO_115050.
    PATRICi23840161. VBISynSp132158_1640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18067.1 .
    PIRi S75506.
    RefSeqi NP_441387.1. NC_000911.1.
    YP_005651444.1. NC_017277.1.
    YP_007451269.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P77969.
    SMRi P77969. Positions 5-326.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P77969. 2 interactions.
    STRINGi 1148.sll1994.

    Proteomic databases

    PaxDbi P77969.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA18067 ; BAA18067 ; BAA18067 .
    GeneIDi 954742.
    KEGGi syn:sll1994.
    syy:SYNGTS_1491.
    syz:MYO_115050.
    PATRICi 23840161. VBISynSp132158_1640.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    KOi K01698.
    OMAi QINPANS.
    OrthoDBi EOG6VXFCB.
    PhylomeDBi P77969.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiHEM2_SYNY3
    AccessioniPrimary (citable) accession number: P77969
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3