Reviewed, UniProtKB/Swiss-Prot P77969 (HEM2_SYNY3)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	sll1994

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Zinc By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Ligand	Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	porphobilinogen synthase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	1	EBI-862729,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Delta-aminolevulinic acid dehydratase		PRO_0000140521
Regions
	Region	116 – 134	19	Zinc-binding By similarity
Sites
	Active site	251	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77969-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: A7FAA7D5915300C9	FASTA	327	36,163
	10 20 30 40 50 60 MFPTIRPRRL RQTDVLRRMV RENTLTVNDL IYPLFAVPGN AIAKEVVSMP GVYQLSVDKI 70 80 90 100 110 120 VDEAKEVRDL GIPAIILFGI PEDKDTDATG AWHDCGIVQK ATEAVKKAVP DLVVIVDTCL 130 140 150 160 170 180 CEYTNHGHCG YLETGDLTGR VLNDPTLELL KKTAVSQANA GADVIAPSGM MDGFVQAIRE 190 200 210 220 230 240 ALDDHDFQNI PILSYAAKYA SAYYGPFRDA ADSSPQFGDR RTYQMDPGNS REALKEVELD 250 260 270 280 290 300 LLEGADMVMV KPALSYMDII WRIKEMTNLP VAAYNVSGEY SMVKAAALNG WIDEQKVTLE 310 320 TLTSFKRAGA DLILTYHAKD AARWLQD

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA18067.1.
PIR	S75506.
RefSeq	NP_441387.1.
3D structure databases
HSSP	HSSP built from PDB template 1B4K based on UniProtKB Q59643.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77969. 2 interactions.
Genome annotation databases
GeneID	954742.
GenomeReviews	Gene locus sll1994 in contig BA000022_GR.
KEGG	syn:sll1994.
NMPDR	fig|1148.1.peg.1488.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P77969.
OMA	P77969. AESTPQF.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1994-MON.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
ProDom	PD002304. AlaD_dehydratase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_SYNY3

Accession

Primary (citable) accession number: P77969

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)