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P77969 (HEM2_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-aminolevulinic acid dehydratase

Short name=ALAD
Short name=ALADH
EC=4.2.1.24
Alternative name(s):
Porphobilinogen synthase
Gene names
Name:hemB
Ordered Locus Names:sll1994
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activity

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactor

Binds 1 zinc ion per monomer By similarity.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.

Subunit structure

Homooctamer By similarity.

Sequence similarities

Belongs to the ALADH family.

Ontologies

Keywords
   Biological processHeme biosynthesis
Porphyrin biosynthesis
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

porphobilinogen synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

trxAP522311EBI-862729,EBI-862916

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327Delta-aminolevulinic acid dehydratase
PRO_0000140521

Sites

Active site1981Schiff-base intermediate with substrate By similarity
Active site2511Schiff-base intermediate with substrate By similarity
Metal binding1191Zinc; catalytic By similarity
Metal binding1211Zinc; catalytic By similarity
Metal binding1291Zinc; catalytic By similarity
Metal binding2361Magnesium By similarity
Binding site2081Substrate 1 By similarity
Binding site2201Substrate 1 By similarity
Binding site2771Substrate 2 By similarity
Binding site3161Substrate 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P77969 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: A7FAA7D5915300C9

FASTA32736,163
        10         20         30         40         50         60 
MFPTIRPRRL RQTDVLRRMV RENTLTVNDL IYPLFAVPGN AIAKEVVSMP GVYQLSVDKI 

        70         80         90        100        110        120 
VDEAKEVRDL GIPAIILFGI PEDKDTDATG AWHDCGIVQK ATEAVKKAVP DLVVIVDTCL 

       130        140        150        160        170        180 
CEYTNHGHCG YLETGDLTGR VLNDPTLELL KKTAVSQANA GADVIAPSGM MDGFVQAIRE 

       190        200        210        220        230        240 
ALDDHDFQNI PILSYAAKYA SAYYGPFRDA ADSSPQFGDR RTYQMDPGNS REALKEVELD 

       250        260        270        280        290        300 
LLEGADMVMV KPALSYMDII WRIKEMTNLP VAAYNVSGEY SMVKAAALNG WIDEQKVTLE 

       310        320 
TLTSFKRAGA DLILTYHAKD AARWLQD 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA18067.1.
PIRS75506.
RefSeqNP_441387.1. NC_000911.1.
YP_005651444.1. NC_017277.1.
YP_007451269.1. NC_020286.1.

3D structure databases

ProteinModelPortalP77969.
SMRP77969. Positions 5-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP77969. 2 interactions.
STRING1148.sll1994.

Proteomic databases

PaxDbP77969.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18067; BAA18067; BAA18067.
GeneID954742.
KEGGsyn:sll1994.
syy:SYNGTS_1491.
syz:MYO_115050.
PATRIC23840161. VBISynSp132158_1640.

Phylogenomic databases

eggNOGCOG0113.
HOGENOMHOG000020323.
KOK01698.
OMAQINPANS.
OrthoDBEOG6VXFCB.
PhylomeDBP77969.

Enzyme and pathway databases

UniPathwayUPA00251; UER00318.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERPTHR11458. PTHR11458. 1 hit.
PfamPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSPR00144. DALDHYDRTASE.
SMARTSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM2_SYNY3
AccessionPrimary (citable) accession number: P77969
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways