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P77969

- HEM2_SYNY3

UniProt

P77969 - HEM2_SYNY3

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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 1 zinc ion per monomer.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191Zinc; catalyticBy similarity
Metal bindingi121 – 1211Zinc; catalyticBy similarity
Metal bindingi129 – 1291Zinc; catalyticBy similarity
Active sitei198 – 1981Schiff-base intermediate with substrateBy similarity
Binding sitei208 – 2081Substrate 1By similarity
Binding sitei220 – 2201Substrate 1By similarity
Metal bindingi236 – 2361MagnesiumBy similarity
Active sitei251 – 2511Schiff-base intermediate with substrateBy similarity
Binding sitei277 – 2771Substrate 2By similarity
Binding sitei316 – 3161Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:sll1994
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Delta-aminolevulinic acid dehydratasePRO_0000140521Add
BLAST

Proteomic databases

PaxDbiP77969.

Interactioni

Subunit structurei

Homooctamer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
trxAP522311EBI-862729,EBI-862916

Protein-protein interaction databases

IntActiP77969. 2 interactions.
STRINGi1148.sll1994.

Structurei

3D structure databases

ProteinModelPortaliP77969.
SMRiP77969. Positions 5-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
InParanoidiP77969.
KOiK01698.
OMAiQINPANS.
OrthoDBiEOG6VXFCB.
PhylomeDBiP77969.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77969-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFPTIRPRRL RQTDVLRRMV RENTLTVNDL IYPLFAVPGN AIAKEVVSMP
60 70 80 90 100
GVYQLSVDKI VDEAKEVRDL GIPAIILFGI PEDKDTDATG AWHDCGIVQK
110 120 130 140 150
ATEAVKKAVP DLVVIVDTCL CEYTNHGHCG YLETGDLTGR VLNDPTLELL
160 170 180 190 200
KKTAVSQANA GADVIAPSGM MDGFVQAIRE ALDDHDFQNI PILSYAAKYA
210 220 230 240 250
SAYYGPFRDA ADSSPQFGDR RTYQMDPGNS REALKEVELD LLEGADMVMV
260 270 280 290 300
KPALSYMDII WRIKEMTNLP VAAYNVSGEY SMVKAAALNG WIDEQKVTLE
310 320
TLTSFKRAGA DLILTYHAKD AARWLQD
Length:327
Mass (Da):36,163
Last modified:February 1, 1997 - v1
Checksum:iA7FAA7D5915300C9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA18067.1.
PIRiS75506.
RefSeqiNP_441387.1. NC_000911.1.
YP_005651444.1. NC_017277.1.
YP_007451269.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA18067; BAA18067; BAA18067.
GeneIDi954742.
KEGGisyn:sll1994.
syy:SYNGTS_1491.
syz:MYO_115050.
PATRICi23840161. VBISynSp132158_1640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA18067.1 .
PIRi S75506.
RefSeqi NP_441387.1. NC_000911.1.
YP_005651444.1. NC_017277.1.
YP_007451269.1. NC_020286.1.

3D structure databases

ProteinModelPortali P77969.
SMRi P77969. Positions 5-326.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P77969. 2 interactions.
STRINGi 1148.sll1994.

Proteomic databases

PaxDbi P77969.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA18067 ; BAA18067 ; BAA18067 .
GeneIDi 954742.
KEGGi syn:sll1994.
syy:SYNGTS_1491.
syz:MYO_115050.
PATRICi 23840161. VBISynSp132158_1640.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
InParanoidi P77969.
KOi K01698.
OMAi QINPANS.
OrthoDBi EOG6VXFCB.
PhylomeDBi P77969.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiHEM2_SYNY3
AccessioniPrimary (citable) accession number: P77969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3