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P77966 (GYRB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit B

EC=5.99.1.3

Cleaved into the following chain:

  1. Ssp gyrB intein
Gene names
Name:gyrB
Ordered Locus Names:sll2005
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length1078 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity.

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA.

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity.

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.

Subcellular location

Cytoplasm Potential.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the type II topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 436436DNA gyrase subunit B, 1st part Potential
PRO_0000034816
Chain437 – 871435Ssp gyrB intein Potential
PRO_0000034817
Chain872 – 1078207DNA gyrase subunit B, 2nd part Potential
PRO_0000034818

Regions

Domain889 – 97486Toprim

Sites

Metal binding8951Magnesium 1; catalytic By similarity
Metal binding9391Magnesium 1; catalytic By similarity
Metal binding9391Magnesium 2 By similarity
Metal binding9411Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P77966 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 6CA02586DFEA607B

FASTA1,078122,819
        10         20         30         40         50         60 
MTMTTTNYGA DQIQVLEGLE PVRKRPGMYI GSTGPKGLHH LVYEVVDNAI DEALAGYCTH 

        70         80         90        100        110        120 
IEIDINADGS VTVVDNGRGI PTDIHPTTGR SALETVLTVL HAGGKFGGGG YKVSGGLHGV 

       130        140        150        160        170        180 
GVSVVNALSE WVEVKVWRQG KEHFQRFERG NPIGTLEATP NEGHSTGTQV SFLPDTQIFK 

       190        200        210        220        230        240 
DGIEFDYHTL ASRLKELAYL NAGVRITFGD RRADSLKEEQ FYYEGGIREY VTYMTTDKTP 

       250        260        270        280        290        300 
LHEEIIYTSG EKNDVQVEVA LQWCVDAYSD TLLGFANNIR TIDGGTHLEG LKAVLTRTLN 

       310        320        330        340        350        360 
SVARKRNKLK DGDSNLGGEN IREGLTGVIS VKVPDPEFEG QTKTKLGNTE VRGIVDTLVG 

       370        380        390        400        410        420 
EALTEFLEFN PGVADAIIEK AVQAFKAAEA ARRARELVRR KSVLESSTLP GKLADCSSKD 

       430        440        450        460        470        480 
PSESEIFIVE GDSAGGCFSG DTLVALTDGR SVSFEQLVEE EKQGKQNFCY TIRHDGSIGV 

       490        500        510        520        530        540 
EKIINARKTK TNAKVIKVTL DNGESIICTP DHKFMLRDGS YKCAMDLTLD DSLMPLHRKI 

       550        560        570        580        590        600 
STTEDSGITI DGYEMVWSPR SDSWLFTHLV ADWYNRWQGI YIAEEKQHCH HKDFNKRNNN 

       610        620        630        640        650        660 
PDNLIRLSPE KHLALHRKHI SKTLHRPDVV EKCRRIHQSP EFRRKMSARM QSPETRAILS 

       670        680        690        700        710        720 
KQAQAQWQNE TYKLTMMESW RSFYDSNEDY RQQNAEQLNR AQQEYWAQAE NRTAQAERVR 

       730        740        750        760        770        780 
QHFAQNPGLR QQYSENAVKQ WNNPELLKWR QKKTKEQWTP EFREKRREAL AQTYYRKTLA 

       790        800        810        820        830        840 
ALKQVEIENG YLDISAYDSY RISTKDKSLL RFDRFCERYF ENDENLAREA VLNYNHRIVN 

       850        860        870        880        890        900 
IEAVSETIDV YDIEVPHTHN FALASGVFVH NSAKQGRDRR FQAILPLRGK ILNIEKTDDA 

       910        920        930        940        950        960 
KIYKNTEIQA LITALGLGIK GDDFDISSLR YHRVVIMTDA DVDGAHIRTL LLTFFYRYQR 

       970        980        990       1000       1010       1020 
DLVDQGYIYI ACPPLYKLER GKNHFYCYSD RELQEQISQF PPNANYTIQR FKGLGEMMPQ 

      1030       1040       1050       1060       1070 
QLWDTTMNPE SRTMKRVHIE DAAEADRIFT VLMGDRVAPR REFIETYGTK LDLTDLDI 

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References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA17720.1.
PIRS77162.
RefSeqNP_441040.1. NC_000911.1.
YP_005651097.1. NC_017277.1.
YP_007450922.1. NC_020286.1.

3D structure databases

ProteinModelPortalP77966.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48809N.
IntActP77966. 15 interactions.
STRING1148.sll2005.

Proteomic databases

PaxDbP77966.
PRIDEP77966.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17720; BAA17720; BAA17720.
GeneID954353.
KEGGsyn:sll2005.
syy:SYNGTS_1144.
syz:MYO_111540.
PATRIC23839370. VBISynSp132158_1249.

Phylogenomic databases

eggNOGCOG0187.
HOGENOMHOG000075154.
KOK02470.
OMANARWREV.
OrthoDBEOG6P334W.
PhylomeDBP77966.

Family and domain databases

Gene3D2.170.16.10. 2 hits.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 2 hits.
InterProIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF51294. SSF51294. 2 hits.
SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 2 hits.
TIGRFAMsTIGR01059. gyrB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGYRB_SYNY3
AccessionPrimary (citable) accession number: P77966
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries