ID   RPOB_SYNY3              Reviewed;        1102 AA.
AC   P77965;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   27-MAR-2024, entry version 145.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN   OrderedLocusNames=sll1787;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales; Merismopediaceae;
OC   Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2
CC       alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma
CC       factor is associated with the core the holoenzyme is formed, which can
CC       initiate transcription. {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; BA000022; BAA17365.1; -; Genomic_DNA.
DR   PIR; S77518; S77518.
DR   PDB; 8GZG; EM; 3.13 A; C=1-1102.
DR   PDB; 8GZH; EM; 2.96 A; C=1-1102.
DR   PDBsum; 8GZG; -.
DR   PDBsum; 8GZH; -.
DR   AlphaFoldDB; P77965; -.
DR   SMR; P77965; -.
DR   DIP; DIP-37767N; -.
DR   IntAct; P77965; 10.
DR   STRING; 1148.gene:10498228; -.
DR   PaxDb; 1148-1652443; -.
DR   EnsemblBacteria; BAA17365; BAA17365; BAA17365.
DR   KEGG; syn:sll1787; -.
DR   eggNOG; COG0085; Bacteria.
DR   InParanoid; P77965; -.
DR   PhylomeDB; P77965; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.50.100; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1100.10; -; 1.
DR   Gene3D; 2.30.150.10; DNA-directed RNA polymerase, beta subunit, external 1 domain; 1.
DR   Gene3D; 2.40.270.10; DNA-directed RNA polymerase, subunit 2, domain 6; 1.
DR   Gene3D; 3.90.1800.10; RNA polymerase alpha subunit dimerisation domain; 1.
DR   Gene3D; 3.90.1110.10; RNA polymerase Rpb2, domain 2; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   NCBIfam; TIGR02013; rpoB; 1.
DR   PANTHER; PTHR20856; DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2; 1.
DR   PANTHER; PTHR20856:SF20; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..1102
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047982"
FT   REGION          1076..1102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           14..25
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           27..35
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          41..43
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          45..49
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           51..53
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   HELIX           63..67
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            68..70
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          74..88
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          92..102
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          110..119
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          121..125
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          127..134
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          136..139
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   STRAND          142..148
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          150..152
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          155..159
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            161..163
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   STRAND          165..169
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           177..184
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           188..194
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           198..208
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           213..224
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           232..242
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            246..248
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           253..263
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           276..288
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           289..291
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          304..308
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           310..335
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           343..346
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           350..361
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          364..368
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           374..380
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          383..385
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            394..396
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   TURN            400..402
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           406..408
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            409..411
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          419..421
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            423..425
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          426..429
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          441..443
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          445..449
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          462..464
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          466..469
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          483..485
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          490..494
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          497..501
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           503..505
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          508..511
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            513..516
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           519..522
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          524..526
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           527..529
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           532..542
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          548..550
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          555..557
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           561..568
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          571..573
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          578..583
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          585..592
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          605..608
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          612..614
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          638..641
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          643..645
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   STRAND          647..650
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   STRAND          653..660
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          668..676
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           677..680
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            681..684
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   STRAND          686..699
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          718..721
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          725..727
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          738..740
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          742..744
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           753..761
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          768..771
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   STRAND          782..786
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          789..791
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           792..794
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          802..814
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          821..823
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          825..827
FT                   /evidence="ECO:0007829|PDB:8GZG"
FT   STRAND          829..836
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            838..840
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          851..854
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           858..862
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           866..880
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           890..893
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           897..910
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          912..914
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          921..923
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            932..934
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          942..953
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           956..958
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          961..965
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          970..972
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            979..982
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           989..997
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           1001..1008
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   TURN            1009..1011
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           1015..1026
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   HELIX           1038..1049
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          1055..1059
FT                   /evidence="ECO:0007829|PDB:8GZH"
FT   STRAND          1064..1066
FT                   /evidence="ECO:0007829|PDB:8GZH"
SQ   SEQUENCE   1102 AA;  123365 MW;  40235F5EBA7CE881 CRC64;
     MTNLATTMLP DLIEIQHASF HWFLEEGLIE ELNSFSPISD YTGKLELHFL GKDYKLKQPK
     YDVDESKRRD ASYSVQMYVP TRLINKETGE IKEQEVFIGD LPLMTERGTF IINGAERVIV
     NQIVRSPGVY YKKELDKNGR RTYSASLIPN RGAWLKFETD KNGLVYVRID KTRKLSAQVL
     LKAIGLSDNE ILDSLSHPEF YQKTLDKEGN PTEEEALVEL YKKLRPGEPP TVSGGQQLLE
     SRFFDPKRYD LGRVGRYKLN KKLRLNEADT TRVLTPQDIL AAINYLINLE FDVGTTDDID
     HLGNRRVRSV GELLQNQIRV GLNRLERIIR ERMTVSESDA LTPASLVNPK PLVAAIKEFF
     GSSQLSQFMD QTNPLAELTH KRRISALGPG GLTRERAGFA VRDIHPSHHG RICPVETPEG
     PNAGLIGSLA TCARVNDYGF IETPYFRVES GRVRKDLDPV YLTADEEDDM RVAPGDIPTD
     EEGNIIGESV PIRYRQEFST TSPEQVDYVA VSPVQIISVA TSMIPFLEHD DANRALMGSN
     MQRQAVPLLR PERPLVGTGL EAQAARDSGM VIVSRTHGIV TYVDATEIRV QPHSPDNPAE
     KGEEIVYPIQ KYQRSNQDTC LNQRPLVYAG EDVVPGQVLA DGSATEGGEL ALGQNILVAY
     MPWEGYNYED AILISERLVY DDVYTSIHIE KFEIEARQTK LGPEEITREI PNVGEDALRN
     LDEHGIIRIG AWVESGDILV GKVTPKGEAD QPPEEKLLRA IFGEKARDVR DNSLRVPNGE
     KGRVVDVRVF TREKGDELPP GANMVVRIYV AQKRKIQVGD KMAGRHGNKG IISRILPIED
     MPYLPDGRPI DIALNPLGVP SRMNVGQVFE CLLGWAGENL GVRFKITPFD EMYGEEASRD
     TVHGLLEEAS QRPNKDWVFN ENHPGKIQVF DGRTGEPFDR PITVGQAYML KLVHLVDDKI
     HARSTGPYSL VTQQPLGGKA QQGGQRFGEM EVWALEAYGA AYILQELLTV KSDDMQGRNE
     ALNAIVKGKS IPRPGTPESF KVLMRELQSL GLDIAAHKVQ LSEDGESADA EVDLMIDSQR
     RAPNRPTYES LHTEEDLEEE EV
//