Reviewed, UniProtKB/Swiss-Prot P77965 (RPOB_SYNY3)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit beta
      Short name=RNAP subunit beta
    EC=2.7.7.6
Alternative name(s):
    Transcriptase subunit beta
    RNA polymerase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	sll1787

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	1102 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	1	EBI-862811,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1102	1102	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047982

Sequences

	Sequence		Length	Mass (Da)
	P77965-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 40235F5EBA7CE881	FASTA	1,102	123,365
	10 20 30 40 50 60 MTNLATTMLP DLIEIQHASF HWFLEEGLIE ELNSFSPISD YTGKLELHFL GKDYKLKQPK 70 80 90 100 110 120 YDVDESKRRD ASYSVQMYVP TRLINKETGE IKEQEVFIGD LPLMTERGTF IINGAERVIV 130 140 150 160 170 180 NQIVRSPGVY YKKELDKNGR RTYSASLIPN RGAWLKFETD KNGLVYVRID KTRKLSAQVL 190 200 210 220 230 240 LKAIGLSDNE ILDSLSHPEF YQKTLDKEGN PTEEEALVEL YKKLRPGEPP TVSGGQQLLE 250 260 270 280 290 300 SRFFDPKRYD LGRVGRYKLN KKLRLNEADT TRVLTPQDIL AAINYLINLE FDVGTTDDID 310 320 330 340 350 360 HLGNRRVRSV GELLQNQIRV GLNRLERIIR ERMTVSESDA LTPASLVNPK PLVAAIKEFF 370 380 390 400 410 420 GSSQLSQFMD QTNPLAELTH KRRISALGPG GLTRERAGFA VRDIHPSHHG RICPVETPEG 430 440 450 460 470 480 PNAGLIGSLA TCARVNDYGF IETPYFRVES GRVRKDLDPV YLTADEEDDM RVAPGDIPTD 490 500 510 520 530 540 EEGNIIGESV PIRYRQEFST TSPEQVDYVA VSPVQIISVA TSMIPFLEHD DANRALMGSN 550 560 570 580 590 600 MQRQAVPLLR PERPLVGTGL EAQAARDSGM VIVSRTHGIV TYVDATEIRV QPHSPDNPAE 610 620 630 640 650 660 KGEEIVYPIQ KYQRSNQDTC LNQRPLVYAG EDVVPGQVLA DGSATEGGEL ALGQNILVAY 670 680 690 700 710 720 MPWEGYNYED AILISERLVY DDVYTSIHIE KFEIEARQTK LGPEEITREI PNVGEDALRN 730 740 750 760 770 780 LDEHGIIRIG AWVESGDILV GKVTPKGEAD QPPEEKLLRA IFGEKARDVR DNSLRVPNGE 790 800 810 820 830 840 KGRVVDVRVF TREKGDELPP GANMVVRIYV AQKRKIQVGD KMAGRHGNKG IISRILPIED 850 860 870 880 890 900 MPYLPDGRPI DIALNPLGVP SRMNVGQVFE CLLGWAGENL GVRFKITPFD EMYGEEASRD 910 920 930 940 950 960 TVHGLLEEAS QRPNKDWVFN ENHPGKIQVF DGRTGEPFDR PITVGQAYML KLVHLVDDKI 970 980 990 1000 1010 1020 HARSTGPYSL VTQQPLGGKA QQGGQRFGEM EVWALEAYGA AYILQELLTV KSDDMQGRNE 1030 1040 1050 1060 1070 1080 ALNAIVKGKS IPRPGTPESF KVLMRELQSL GLDIAAHKVQ LSEDGESADA EVDLMIDSQR 1090 1100 RAPNRPTYES LHTEEDLEEE EV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA17365.1.
PIR	S77518.
RefSeq	NP_440685.1.
3D structure databases
HSSP	HSSP built from PDB template 1HQM based on UniProtKB Q9KWU7.
ModBase	Search...
Protein-protein interaction databases
IntAct	P77965. 9 interactions.
Genome annotation databases
GeneID	953988.
GenomeReviews	Gene locus sll1787 in contig BA000022_GR.
KEGG	syn:sll1787.
NMPDR	fig|1148.1.peg.786.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P77965.
OMA	P77965. PVRYRQD.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1787-MON.
Family and domain databases
HAMAP	MF_01321. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. rpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_SYNY3

Accession

Primary (citable) accession number: P77965

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)