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Protein

Glutamine synthetase

Gene

glnA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulationi

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 382.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:glnA
Ordered Locus Names:slr1756
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532721 – 473Glutamine synthetaseAdd BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei401O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP77961.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

DIPiDIP-48822N.
IntActiP77961. 4 interactors.

Structurei

Secondary structure

1473
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 14Combined sources10
Beta strandi19 – 25Combined sources7
Beta strandi27 – 29Combined sources3
Beta strandi31 – 37Combined sources7
Helixi38 – 40Combined sources3
Helixi43 – 46Combined sources4
Beta strandi50 – 53Combined sources4
Helixi63 – 65Combined sources3
Beta strandi67 – 72Combined sources6
Helixi74 – 76Combined sources3
Beta strandi87 – 95Combined sources9
Turni97 – 99Combined sources3
Beta strandi100 – 102Combined sources3
Helixi107 – 120Combined sources14
Beta strandi121 – 123Combined sources3
Beta strandi125 – 133Combined sources9
Beta strandi135 – 145Combined sources11
Beta strandi150 – 155Combined sources6
Helixi160 – 162Combined sources3
Turni186 – 188Combined sources3
Helixi192 – 204Combined sources13
Beta strandi209 – 214Combined sources6
Turni218 – 220Combined sources3
Beta strandi221 – 226Combined sources6
Helixi231 – 251Combined sources21
Beta strandi255 – 257Combined sources3
Beta strandi271 – 279Combined sources9
Beta strandi286 – 289Combined sources4
Helixi290 – 292Combined sources3
Helixi295 – 315Combined sources21
Helixi321 – 324Combined sources4
Turni327 – 329Combined sources3
Beta strandi337 – 340Combined sources4
Beta strandi344 – 348Combined sources5
Helixi355 – 357Combined sources3
Beta strandi360 – 362Combined sources3
Helixi371 – 388Combined sources18
Helixi418 – 427Combined sources10
Helixi430 – 433Combined sources4
Beta strandi436 – 438Combined sources3
Helixi440 – 453Combined sources14
Helixi455 – 460Combined sources6
Helixi465 – 470Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NG0X-ray2.80A1-473[»]
ProteinModelPortaliP77961.
SMRiP77961.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

HOGENOMiHOG000005157.
InParanoidiP77961.
KOiK01915.
PhylomeDBiP77961.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTPQEVLK WIQDENIKII DLKFIDTPGI WQHCSFYYDQ LDENSFTEGI
60 70 80 90 100
PFDGSSIRGW KAINESDMCM VPDPNTATID PFCKEPTLSM ICSIKEPRTG
110 120 130 140 150
EWYNRDPRTI AAKAVEYLRG TGIADTVYFG PEAEFFLFDD IRFGQTENSS
160 170 180 190 200
YYFADSVEGR WNTGREEEGG NLGYKPGYKQ GYFPVAPTDT AQDIRTEMLL
210 220 230 240 250
TMAGLCVPIE KHHHEVASGG QNELGIKFDK LVNSADNLMI YKYVIKNVAK
260 270 280 290 300
KYGKTVTFMP KPIFNDNGSG MHVHQSLWKD GQPLFAGDKY AGFSQMGLWY
310 320 330 340 350
IGGILKHAPA LLAFTNPTTN SYKRLVPGFE APVNLAYSQG NRSASVRIPL
360 370 380 390 400
SGGNPKAKRL EFRCPDATSN PYLAFAAMLC AGIDGIKNQI DPGEPLDVDI
410 420 430 440 450
YDLSPEELAK IPSTPGSLEA ALEALEKDHE FLTGTGVFSP DFVESWIEYK
460 470
LDNEVNPMRL RPHPYEFSLY YDC
Length:473
Mass (Da):53,026
Last modified:November 1, 1997 - v2
Checksum:i75F8E28EB5EA9515
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115V → A in BAA17055 (PubMed:8905231).Curated1
Sequence conflicti145 – 147QTE → PNG in CAA49139 (PubMed:7727755).Curated3
Sequence conflicti180Q → E in CAA49139 (PubMed:7727755).Curated1
Sequence conflicti204 – 206GLC → AFG in BAA17055 (PubMed:8905231).Curated3
Sequence conflicti230K → KFDK (PubMed:7727755).Curated1
Sequence conflicti239M → I in CAA49139 (PubMed:7727755).Curated1
Sequence conflicti358K → N in CAA49139 (PubMed:7727755).Curated1
Sequence conflicti378 – 379ML → IV in CAA49139 (PubMed:7727755).Curated2
Sequence conflicti430E → Q in CAA49139 (PubMed:7727755).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69199 Genomic DNA. Translation: CAA49139.1.
BA000022 Genomic DNA. Translation: BAA17055.1.
PIRiS75141.

Genome annotation databases

EnsemblBacteriaiBAA17055; BAA17055; BAA17055.
KEGGisyn:slr1756.
PATRICi23837908. VBISynSp132158_0523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69199 Genomic DNA. Translation: CAA49139.1.
BA000022 Genomic DNA. Translation: BAA17055.1.
PIRiS75141.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NG0X-ray2.80A1-473[»]
ProteinModelPortaliP77961.
SMRiP77961.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48822N.
IntActiP77961. 4 interactors.

Proteomic databases

PRIDEiP77961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17055; BAA17055; BAA17055.
KEGGisyn:slr1756.
PATRICi23837908. VBISynSp132158_0523.

Phylogenomic databases

HOGENOMiHOG000005157.
InParanoidiP77961.
KOiK01915.
PhylomeDBiP77961.

Enzyme and pathway databases

BRENDAi6.3.1.2. 382.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA_SYNY3
AccessioniPrimary (citable) accession number: P77961
Secondary accession number(s): Q59981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.