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P77961 (GLNA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
Ordered Locus Names:slr1756
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulation

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

Subunit structure

Oligomer of 12 subunits arranged in the form of two hexagons By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 473473Glutamine synthetase
PRO_0000153272

Amino acid modifications

Modified residue4011O-AMP-tyrosine By similarity

Experimental info

Sequence conflict1151V → A in BAA17055. Ref.2
Sequence conflict145 – 1473QTE → PNG in CAA49139. Ref.1
Sequence conflict1801Q → E in CAA49139. Ref.1
Sequence conflict204 – 2063GLC → AFG in BAA17055. Ref.2
Sequence conflict2301K → KFDK Ref.1
Sequence conflict2391M → I in CAA49139. Ref.1
Sequence conflict3581K → N in CAA49139. Ref.1
Sequence conflict378 – 3792ML → IV in CAA49139. Ref.1
Sequence conflict4301E → Q in CAA49139. Ref.1

Secondary structure

.................................................................................. 473
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P77961 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 75F8E28EB5EA9515

FASTA47353,026
        10         20         30         40         50         60 
MARTPQEVLK WIQDENIKII DLKFIDTPGI WQHCSFYYDQ LDENSFTEGI PFDGSSIRGW 

        70         80         90        100        110        120 
KAINESDMCM VPDPNTATID PFCKEPTLSM ICSIKEPRTG EWYNRDPRTI AAKAVEYLRG 

       130        140        150        160        170        180 
TGIADTVYFG PEAEFFLFDD IRFGQTENSS YYFADSVEGR WNTGREEEGG NLGYKPGYKQ 

       190        200        210        220        230        240 
GYFPVAPTDT AQDIRTEMLL TMAGLCVPIE KHHHEVASGG QNELGIKFDK LVNSADNLMI 

       250        260        270        280        290        300 
YKYVIKNVAK KYGKTVTFMP KPIFNDNGSG MHVHQSLWKD GQPLFAGDKY AGFSQMGLWY 

       310        320        330        340        350        360 
IGGILKHAPA LLAFTNPTTN SYKRLVPGFE APVNLAYSQG NRSASVRIPL SGGNPKAKRL 

       370        380        390        400        410        420 
EFRCPDATSN PYLAFAAMLC AGIDGIKNQI DPGEPLDVDI YDLSPEELAK IPSTPGSLEA 

       430        440        450        460        470 
ALEALEKDHE FLTGTGVFSP DFVESWIEYK LDNEVNPMRL RPHPYEFSLY YDC 

« Hide

References

« Hide 'large scale' references
[1]"Electron transport controls transcription of the glutamine synthetase gene (glnA) from the cyanobacterium Synechocystis sp. PCC 6803."
Reyes J.C., Florencio F.J.
Plant Mol. Biol. 27:789-799(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69199 Genomic DNA. Translation: CAA49139.1.
BA000022 Genomic DNA. Translation: BAA17055.1.
PIRS75141.
RefSeqNP_440375.1. NC_000911.1.
YP_005650432.1. NC_017277.1.
YP_007450259.1. NC_020286.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3NG0X-ray2.80A1-473[»]
ProteinModelPortalP77961.
SMRP77961. Positions 2-473.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48822N.
IntActP77961. 4 interactions.
STRING1148.slr1756.

Proteomic databases

PaxDbP77961.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17055; BAA17055; BAA17055.
GeneID953675.
KEGGsyn:slr1756.
syy:SYNGTS_0479.
syz:MYO_14850.
PATRIC23837908. VBISynSp132158_0523.

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000005157.
KOK01915.
OrthoDBEOG6B360N.
PhylomeDBP77961.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_SYNY3
AccessionPrimary (citable) accession number: P77961
Secondary accession number(s): Q59981
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references