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P77961

- GLNA_SYNY3

UniProt

P77961 - GLNA_SYNY3

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Protein

Glutamine synthetase

Gene

glnA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulationi

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:glnA
Ordered Locus Names:slr1756
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Glutamine synthetasePRO_0000153272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei401 – 4011O-AMP-tyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP77961.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

DIPiDIP-48822N.
IntActiP77961. 4 interactions.
STRINGi1148.slr1756.

Structurei

Secondary structure

1
473
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1410
Beta strandi19 – 257
Beta strandi27 – 293
Beta strandi31 – 377
Helixi38 – 403
Helixi43 – 464
Beta strandi50 – 534
Helixi63 – 653
Beta strandi67 – 726
Helixi74 – 763
Beta strandi87 – 959
Turni97 – 993
Beta strandi100 – 1023
Helixi107 – 12014
Beta strandi121 – 1233
Beta strandi125 – 1339
Beta strandi135 – 14511
Beta strandi150 – 1556
Helixi160 – 1623
Turni186 – 1883
Helixi192 – 20413
Beta strandi209 – 2146
Turni218 – 2203
Beta strandi221 – 2266
Helixi231 – 25121
Beta strandi255 – 2573
Beta strandi271 – 2799
Beta strandi286 – 2894
Helixi290 – 2923
Helixi295 – 31521
Helixi321 – 3244
Turni327 – 3293
Beta strandi337 – 3404
Beta strandi344 – 3485
Helixi355 – 3573
Beta strandi360 – 3623
Helixi371 – 38818
Helixi418 – 42710
Helixi430 – 4334
Beta strandi436 – 4383
Helixi440 – 45314
Helixi455 – 4606
Helixi465 – 4706

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NG0X-ray2.80A1-473[»]
ProteinModelPortaliP77961.
SMRiP77961. Positions 2-473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000005157.
InParanoidiP77961.
KOiK01915.
OrthoDBiEOG6B360N.
PhylomeDBiP77961.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77961-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARTPQEVLK WIQDENIKII DLKFIDTPGI WQHCSFYYDQ LDENSFTEGI
60 70 80 90 100
PFDGSSIRGW KAINESDMCM VPDPNTATID PFCKEPTLSM ICSIKEPRTG
110 120 130 140 150
EWYNRDPRTI AAKAVEYLRG TGIADTVYFG PEAEFFLFDD IRFGQTENSS
160 170 180 190 200
YYFADSVEGR WNTGREEEGG NLGYKPGYKQ GYFPVAPTDT AQDIRTEMLL
210 220 230 240 250
TMAGLCVPIE KHHHEVASGG QNELGIKFDK LVNSADNLMI YKYVIKNVAK
260 270 280 290 300
KYGKTVTFMP KPIFNDNGSG MHVHQSLWKD GQPLFAGDKY AGFSQMGLWY
310 320 330 340 350
IGGILKHAPA LLAFTNPTTN SYKRLVPGFE APVNLAYSQG NRSASVRIPL
360 370 380 390 400
SGGNPKAKRL EFRCPDATSN PYLAFAAMLC AGIDGIKNQI DPGEPLDVDI
410 420 430 440 450
YDLSPEELAK IPSTPGSLEA ALEALEKDHE FLTGTGVFSP DFVESWIEYK
460 470
LDNEVNPMRL RPHPYEFSLY YDC
Length:473
Mass (Da):53,026
Last modified:November 1, 1997 - v2
Checksum:i75F8E28EB5EA9515
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151V → A in BAA17055. (PubMed:8905231)Curated
Sequence conflicti145 – 1473QTE → PNG in CAA49139. (PubMed:7727755)Curated
Sequence conflicti180 – 1801Q → E in CAA49139. (PubMed:7727755)Curated
Sequence conflicti204 – 2063GLC → AFG in BAA17055. (PubMed:8905231)Curated
Sequence conflicti230 – 2301K → KFDK(PubMed:7727755)Curated
Sequence conflicti239 – 2391M → I in CAA49139. (PubMed:7727755)Curated
Sequence conflicti358 – 3581K → N in CAA49139. (PubMed:7727755)Curated
Sequence conflicti378 – 3792ML → IV in CAA49139. (PubMed:7727755)Curated
Sequence conflicti430 – 4301E → Q in CAA49139. (PubMed:7727755)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69199 Genomic DNA. Translation: CAA49139.1.
BA000022 Genomic DNA. Translation: BAA17055.1.
PIRiS75141.
RefSeqiNP_440375.1. NC_000911.1.
YP_005650432.1. NC_017277.1.
YP_007450259.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17055; BAA17055; BAA17055.
GeneIDi953675.
KEGGisyn:slr1756.
syy:SYNGTS_0479.
syz:MYO_14850.
PATRICi23837908. VBISynSp132158_0523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69199 Genomic DNA. Translation: CAA49139.1 .
BA000022 Genomic DNA. Translation: BAA17055.1 .
PIRi S75141.
RefSeqi NP_440375.1. NC_000911.1.
YP_005650432.1. NC_017277.1.
YP_007450259.1. NC_020286.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3NG0 X-ray 2.80 A 1-473 [» ]
ProteinModelPortali P77961.
SMRi P77961. Positions 2-473.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48822N.
IntActi P77961. 4 interactions.
STRINGi 1148.slr1756.

Proteomic databases

PaxDbi P77961.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA17055 ; BAA17055 ; BAA17055 .
GeneIDi 953675.
KEGGi syn:slr1756.
syy:SYNGTS_0479.
syz:MYO_14850.
PATRICi 23837908. VBISynSp132158_0523.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000005157.
InParanoidi P77961.
KOi K01915.
OrthoDBi EOG6B360N.
PhylomeDBi P77961.

Family and domain databases

Gene3Di 3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
TIGRFAMsi TIGR00653. GlnA. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Electron transport controls transcription of the glutamine synthetase gene (glnA) from the cyanobacterium Synechocystis sp. PCC 6803."
    Reyes J.C., Florencio F.J.
    Plant Mol. Biol. 27:789-799(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiGLNA_SYNY3
AccessioniPrimary (citable) accession number: P77961
Secondary accession number(s): Q59981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3