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P77961

- GLNA_SYNY3

UniProt

P77961 - GLNA_SYNY3

Protein

Glutamine synthetase

Gene

glnA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Enzyme regulationi

    The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase
    Gene namesi
    Name:glnA
    Ordered Locus Names:slr1756
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Glutamine synthetasePRO_0000153272Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei401 – 4011O-AMP-tyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP77961.

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

    Protein-protein interaction databases

    DIPiDIP-48822N.
    IntActiP77961. 4 interactions.
    STRINGi1148.slr1756.

    Structurei

    Secondary structure

    1
    473
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 1410
    Beta strandi19 – 257
    Beta strandi27 – 293
    Beta strandi31 – 377
    Helixi38 – 403
    Helixi43 – 464
    Beta strandi50 – 534
    Helixi63 – 653
    Beta strandi67 – 726
    Helixi74 – 763
    Beta strandi87 – 959
    Turni97 – 993
    Beta strandi100 – 1023
    Helixi107 – 12014
    Beta strandi121 – 1233
    Beta strandi125 – 1339
    Beta strandi135 – 14511
    Beta strandi150 – 1556
    Helixi160 – 1623
    Turni186 – 1883
    Helixi192 – 20413
    Beta strandi209 – 2146
    Turni218 – 2203
    Beta strandi221 – 2266
    Helixi231 – 25121
    Beta strandi255 – 2573
    Beta strandi271 – 2799
    Beta strandi286 – 2894
    Helixi290 – 2923
    Helixi295 – 31521
    Helixi321 – 3244
    Turni327 – 3293
    Beta strandi337 – 3404
    Beta strandi344 – 3485
    Helixi355 – 3573
    Beta strandi360 – 3623
    Helixi371 – 38818
    Helixi418 – 42710
    Helixi430 – 4334
    Beta strandi436 – 4383
    Helixi440 – 45314
    Helixi455 – 4606
    Helixi465 – 4706

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3NG0X-ray2.80A1-473[»]
    ProteinModelPortaliP77961.
    SMRiP77961. Positions 2-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000005157.
    KOiK01915.
    OrthoDBiEOG6B360N.
    PhylomeDBiP77961.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P77961-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARTPQEVLK WIQDENIKII DLKFIDTPGI WQHCSFYYDQ LDENSFTEGI    50
    PFDGSSIRGW KAINESDMCM VPDPNTATID PFCKEPTLSM ICSIKEPRTG 100
    EWYNRDPRTI AAKAVEYLRG TGIADTVYFG PEAEFFLFDD IRFGQTENSS 150
    YYFADSVEGR WNTGREEEGG NLGYKPGYKQ GYFPVAPTDT AQDIRTEMLL 200
    TMAGLCVPIE KHHHEVASGG QNELGIKFDK LVNSADNLMI YKYVIKNVAK 250
    KYGKTVTFMP KPIFNDNGSG MHVHQSLWKD GQPLFAGDKY AGFSQMGLWY 300
    IGGILKHAPA LLAFTNPTTN SYKRLVPGFE APVNLAYSQG NRSASVRIPL 350
    SGGNPKAKRL EFRCPDATSN PYLAFAAMLC AGIDGIKNQI DPGEPLDVDI 400
    YDLSPEELAK IPSTPGSLEA ALEALEKDHE FLTGTGVFSP DFVESWIEYK 450
    LDNEVNPMRL RPHPYEFSLY YDC 473
    Length:473
    Mass (Da):53,026
    Last modified:November 1, 1997 - v2
    Checksum:i75F8E28EB5EA9515
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1151V → A in BAA17055. (PubMed:8905231)Curated
    Sequence conflicti145 – 1473QTE → PNG in CAA49139. (PubMed:7727755)Curated
    Sequence conflicti180 – 1801Q → E in CAA49139. (PubMed:7727755)Curated
    Sequence conflicti204 – 2063GLC → AFG in BAA17055. (PubMed:8905231)Curated
    Sequence conflicti230 – 2301K → KFDK(PubMed:7727755)Curated
    Sequence conflicti239 – 2391M → I in CAA49139. (PubMed:7727755)Curated
    Sequence conflicti358 – 3581K → N in CAA49139. (PubMed:7727755)Curated
    Sequence conflicti378 – 3792ML → IV in CAA49139. (PubMed:7727755)Curated
    Sequence conflicti430 – 4301E → Q in CAA49139. (PubMed:7727755)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69199 Genomic DNA. Translation: CAA49139.1.
    BA000022 Genomic DNA. Translation: BAA17055.1.
    PIRiS75141.
    RefSeqiNP_440375.1. NC_000911.1.
    YP_005650432.1. NC_017277.1.
    YP_007450259.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA17055; BAA17055; BAA17055.
    GeneIDi953675.
    KEGGisyn:slr1756.
    syy:SYNGTS_0479.
    syz:MYO_14850.
    PATRICi23837908. VBISynSp132158_0523.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69199 Genomic DNA. Translation: CAA49139.1 .
    BA000022 Genomic DNA. Translation: BAA17055.1 .
    PIRi S75141.
    RefSeqi NP_440375.1. NC_000911.1.
    YP_005650432.1. NC_017277.1.
    YP_007450259.1. NC_020286.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3NG0 X-ray 2.80 A 1-473 [» ]
    ProteinModelPortali P77961.
    SMRi P77961. Positions 2-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48822N.
    IntActi P77961. 4 interactions.
    STRINGi 1148.slr1756.

    Proteomic databases

    PaxDbi P77961.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA17055 ; BAA17055 ; BAA17055 .
    GeneIDi 953675.
    KEGGi syn:slr1756.
    syy:SYNGTS_0479.
    syz:MYO_14850.
    PATRICi 23837908. VBISynSp132158_0523.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000005157.
    KOi K01915.
    OrthoDBi EOG6B360N.
    PhylomeDBi P77961.

    Family and domain databases

    Gene3Di 3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    TIGRFAMsi TIGR00653. GlnA. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Electron transport controls transcription of the glutamine synthetase gene (glnA) from the cyanobacterium Synechocystis sp. PCC 6803."
      Reyes J.C., Florencio F.J.
      Plant Mol. Biol. 27:789-799(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiGLNA_SYNY3
    AccessioniPrimary (citable) accession number: P77961
    Secondary accession number(s): Q59981
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3