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Protein

Glutamine synthetase

Gene

glnA

Organism
Streptomyces filamentosus (Streptomyces roseosporus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi129Magnesium 1By similarity1
Metal bindingi131Magnesium 2By similarity1
Binding sitei205ATPBy similarity1
Metal bindingi210Magnesium 2By similarity1
Metal bindingi218Magnesium 2By similarity1
Binding sitei263L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi267Magnesium 1; via pros nitrogenBy similarity1
Binding sitei271ATPBy similarity1
Binding sitei320L-glutamateBy similarity1
Binding sitei326L-glutamateBy similarity1
Binding sitei338ATPBy similarity1
Binding sitei338L-glutamateBy similarity1
Binding sitei343ATPBy similarity1
Binding sitei352ATPBy similarity1
Metal bindingi357Magnesium 1By similarity1
Binding sitei359L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 223ATPBy similarity3
Nucleotide bindingi269 – 271ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
OrganismiStreptomyces filamentosus (Streptomyces roseosporus)
Taxonomic identifieri67294 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532681 – 469Glutamine synthetaseAdd BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei397O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Structurei

3D structure databases

ProteinModelPortaliP77958.
SMRiP77958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 263L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P77958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQNADEVQK YVADNDVKFI DVRFCDLPGV MQHFTIPAAT FDPAEELAFD
60 70 80 90 100
GSSIRGFQAI HESDMALRAD LSTARVDPFR RDKTININFF IHDPITGEQY
110 120 130 140 150
SRDPRNIAKK AEAYLASTGI ADTAYFGPEA EFYVFDNVRF QTSANESFYH
160 170 180 190 200
IDSEAGAWNT GAVENNRGYK VRYKGGYFPA PPVDHFADLR AEISLELDKN
210 220 230 240 250
GLQVERQHHE VGTAGQAEIN YKFNTLLAAA DDLMLFKYIV KNVAWRNGKT
260 270 280 290 300
ATFMPKPIFG DNGSGMHVHQ SLWQGGSPLF YDEQGYAGLS DTARYYIGGI
310 320 330 340 350
LKHAPSLLAF TNPTVNSYHR LVPGFEAPVN MVYSQRNRSA AMRIPITGSN
360 370 380 390 400
PKAKRVEFRA PDPSSNPYLA FSALLMAGLD GVKNKIEPAE PIDKDLYELA
410 420 430 440 450
PEEHANVQQV PTSLPAVLDA LEADNEYLQA GGVFTSDLIE TWIDYKRTNE
460
IAPIQLRPHP HEFELYFDI
Length:469
Mass (Da):52,514
Last modified:February 1, 1997 - v1
Checksum:i9AE0FF7A6675F792
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58138 Genomic DNA. Translation: AAB41959.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1B_STRFL
AccessioniPrimary (citable) accession number: P77958
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 12, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families