P77952 (GLSA_STRGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-glutamine:scyllo-inosose aminotransferase
EC=2.6.1.50

Alternative name(s):
Glutamine--scyllo-inositol transaminase

Gene names

Name:	stsC
ORF Names:	SG7F10.11c

Organism

Streptomyces griseus

Taxonomic identifier

1911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces griseus group

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the PLP-dependent transamination of scyllo-inosose to form scyllo-inosamine. Ref.1
Catalytic activity	L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone = 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol.
Cofactor	Pyridoxal phosphate. Ref.1
Pathway	Antibiotic biosynthesis; streptomycin biosynthesis.
Subunit structure	Homodimer. Ref.1
Sequence similarities	Belongs to the DegT/DnrJ/EryC1 family. L-glutamine:2-deoxy-scyllo-inosose/scyllo-inosose aminotransferase subfamily.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Gene Ontology (GO)
Biological_process	streptomycin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	L-glutamine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC glutamine-scyllo-inositol transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	L-glutamine:scyllo-inosose aminotransferase		PRO_0000233018
Amino acid modifications
	Modified residue	202	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77952 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: AED73E61EA9B0E33	FASTA	424	45,501
	10 20 30 40 50 60 MDSSLAISGG PRLSNREWPR WPQPGDRALK SLEDVLTSGR WTISCAYQGR DSYERQFASA 70 80 90 100 110 120 FADYCGSAMC VPISTGTASL AIALEACGVG AGDEVIVPGL SWVASASAVL GINAVPVLVD 130 140 150 160 170 180 VDPATYCLDP AATEAAITER TRAITVVHAY SAVADLDALL DIARRHGLPL IEDCAHAHGA 190 200 210 220 230 240 GFRGRPVGAH GAAGVFSMQG SKLLTCGEGG ALVTDDADVA LRAEHLRADG RVVRREPVGV 250 260 270 280 290 300 GEMELEETGR MMGSNACLSE FHAAVLLDQL ELLDGQNARR TRAADHLTDR LSELGMTAQA 310 320 330 340 350 360 TAPGTTARAY YRYLVRLPDE VLAVAPVERF AHALTAELGF AVTQTHRPLN DNPLNRPSSR 370 380 390 400 410 420 RRFATDARYL ERVDPSRFDL PAAKRAHESV VSFSHEVLLA PLDAIDDIAR AFRKVLDNVR EVSR

References

[1]

"Identification of stsC, the gene encoding the L-glutamine:scyllo-inosose aminotransferase from streptomycin-producing Streptomycetes." Ahlert J., Distler J., Mansouri K., Piepersberg W. Arch. Microbiol. 168:102-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CHARACTERIZATION, COFACTOR, SUBUNIT. Strain: N2-3-11.

[2]

"Cloning and heterologous expression of the str/sts-gene cluster from a Streptomyces griseus DSM40236 PAC library: the cluster for streptomycin production lies in a highly conserved genomic area." van der Geize R., Dijkhuizen L., Wellington E.M., Piepersberg W. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23345 / DSM 40236 / JCM 4644 / NBRC 12875 / NCIMB 13023 / NRRL B-2682 / VKM Ac-800.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08763 Genomic DNA. Translation: CAA70012.1. AJ862840 Genomic DNA. Translation: CAH94315.1.
3D structure databases
ProteinModelPortal	P77952.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14011.
UniPathway	UPA00066.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR000653. DegT/StrS_aminotransferase. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF01041. DegT_DnrJ_EryC1. 1 hit. [Graphical view]
PIRSF	PIRSF000390. PLP_StrS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA_STRGR

Accession

Primary (citable) accession number: P77952
Secondary accession number(s): Q53IE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program