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Protein

Malate synthase

Gene

aceB

Organism
Streptomyces arenae
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway:iglyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase (aceB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase (EC:2.3.3.9)
Gene namesi
Name:aceB
OrganismiStreptomyces arenae
Taxonomic identifieri29301 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Malate synthasePRO_0000166877Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP77947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPAPSPLA IVDAEPLPRQ EEVLTDAALA FVAELLHRRF TRAVTNSSPR
60 70 80 90 100
RADAAREIAR TSTLDFLPET AAVRADDSWR VAGPAALNDR RVEITGPTDR
110 120 130 140 150
KMTINALNSG AKVWLADFEE PSAPTWENVV LGQVNLSDAY TRNIDFTDER
160 170 180 190 200
TGKTYALRPL KSWRPSSWPR GWHLNERHLV DPDGSFGARR VVVDFGLYFF
210 220 230 240 250
HNAQRLLDLG KGPYFYLPKT ESHLEARLWN DVFVFAQDYV GIPQGTVRAT
260 270 280 290 300
VLIETITAAY EMEEILYELR DHASGLNAGR WDYLFSIVKN FPATRPKFVL
310 320 330 340 350
PDRNVTMTKL TMTAFMRRYT NCSSYCHNAR ASAAIGGMAA FIPSRDAEVN
360 370 380 390 400
KVAFEKVRAD KDREANDGFD GSWVAHPDLV PIAMESFDRC WHRPNQKDRL
410 420 430 440 450
REDVHVEAAD LIAVDSLEAT TYAGSSSTAV QVGIRYIEAW LRGLGRVAIF
460 470 480 490 500
NLMEDAATAE ISRSQIWQWI NAGVEFEHGE KATPIWPRKS RRGRKWRPVR
510 520 530 540
EELGEEPFAA RDWQHAHDLV VQVSLDEDYA DFLTLPAYER LRG
Length:543
Mass (Da):61,408
Last modified:February 1, 1997 - v1
Checksum:i60829A40F62A0D85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63518 Genomic DNA. Translation: AAB53938.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63518 Genomic DNA. Translation: AAB53938.1.

3D structure databases

ProteinModelPortaliP77947.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Gene cloning and sequencing, and enzyme purification of the malate synthase of Streptomyces arenae."
    Huettner S., Mecke D., Froehlich K.-U.
    Gene 188:239-246(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: Tu469.

Entry informationi

Entry nameiMASY_STRAE
AccessioniPrimary (citable) accession number: P77947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.