Reviewed, UniProtKB/Swiss-Prot P77947 (MASY_STRAE)

Names and origin

Protein names

Recommended name:
    Malate synthase
    EC=2.3.3.9

Gene names

Name:

aceB

Organism

Streptomyces arenae

Taxonomic identifier

29301 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Malate synthase		PRO_0000166877

Sequences

	Sequence		Length	Mass (Da)
	P77947-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 60829A40F62A0D85	FASTA	543	61,408
	10 20 30 40 50 60 MSAPAPSPLA IVDAEPLPRQ EEVLTDAALA FVAELLHRRF TRAVTNSSPR RADAAREIAR 70 80 90 100 110 120 TSTLDFLPET AAVRADDSWR VAGPAALNDR RVEITGPTDR KMTINALNSG AKVWLADFEE 130 140 150 160 170 180 PSAPTWENVV LGQVNLSDAY TRNIDFTDER TGKTYALRPL KSWRPSSWPR GWHLNERHLV 190 200 210 220 230 240 DPDGSFGARR VVVDFGLYFF HNAQRLLDLG KGPYFYLPKT ESHLEARLWN DVFVFAQDYV 250 260 270 280 290 300 GIPQGTVRAT VLIETITAAY EMEEILYELR DHASGLNAGR WDYLFSIVKN FPATRPKFVL 310 320 330 340 350 360 PDRNVTMTKL TMTAFMRRYT NCSSYCHNAR ASAAIGGMAA FIPSRDAEVN KVAFEKVRAD 370 380 390 400 410 420 KDREANDGFD GSWVAHPDLV PIAMESFDRC WHRPNQKDRL REDVHVEAAD LIAVDSLEAT 430 440 450 460 470 480 TYAGSSSTAV QVGIRYIEAW LRGLGRVAIF NLMEDAATAE ISRSQIWQWI NAGVEFEHGE 490 500 510 520 530 540 KATPIWPRKS RRGRKWRPVR EELGEEPFAA RDWQHAHDLV VQVSLDEDYA DFLTLPAYER LRG

References

[1]	"Gene cloning and sequencing, and enzyme purification of the malate synthase of Streptomyces arenae." Huettner S., Mecke D., Froehlich K.-U. Gene 188:239-246(1997) [PubMed: 9133598] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: Tu469.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U63518 Genomic DNA. Translation: AAB53938.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.3.9. 191182.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_STRAE

Accession

Primary (citable) accession number: P77947

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	September 22, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)