Reviewed, UniProtKB/Swiss-Prot P77935 (PUR1_RHIET)

Names and origin

Protein names

Recommended name:
    Amidophosphoribosyltransferase
      Short name=ATase
    EC=2.4.2.14
Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
      Short name=GPATase

Gene names

Name:

purF

Organism

Rhizobium etli

Taxonomic identifier

29449 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 21	21	By similarity		PRO_0000029261
	Chain	22 – 498	477	Amidophosphoribosyltransferase		PRO_0000029262
Regions
	Domain	22 – 241	220	Glutamine amidotransferase type-2
Sites
	Active site	22	1	For GATase activity By similarity
	Metal binding	373	1	Magnesium By similarity
	Metal binding	374	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77935-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 6BF1451CD2DF979D	FASTA	498	54,439
	10 20 30 40 50 60 MNQSHSFPTD DPLDGDTLHE ECGVFGILGH PDAAALTALG LHALQHRGQE AAGIVSFDGK 70 80 90 100 110 120 RFYQERHMGL VGDHYTNPMT LARLPGSISI GHTRYSTTGE VAMRNVQPLF AELEEGGIAI 130 140 150 160 170 180 AHNGNFTNGL TLRRQIIATG AICQSTSDTE VVLHLIARSR HASTSDRFID AIRQMEGGYS 190 200 210 220 230 240 MLAMTRTKLI AASHPTGIRP LVMGELDGKP IFCSETCALD IIGAKFIRDV ENGEVIICEI 250 260 270 280 290 300 QPDGSISIDA RKPSKPQPER LCLFEYVYFA RPDSVVGGRN VYTTRKNMGM NLAKESPVDA 310 320 330 340 350 360 DVVVPVPDGG TSAAGGYAQE SGIPFEYEYG IIRNHYVGRT FIEPTQQIRA FGVKLKHSAN 370 380 390 400 410 420 RAMIEGKRVV LVDDSIVRGT TSLKIVQMIR EAGAREVHIR VASPMIFFRD FYGSIRPTPD 430 440 450 460 470 480 KLLANQYADV EAMAKYIGAD SLAFLSINGL YRAVGGEDRN PARPQFTDHY FTGDYPTRLL 490 DKNGESMGNK LSMLASNG

References

[1]	Soberon M., Lopez O., Girard L., Miranda J., Morera C. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CE3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U65392 Genomic DNA. Translation: AAB06461.1.
3D structure databases
HSSP	HSSP built from PDB template 1AO0 based on UniProtKB P00497.
ModBase	Search...
Protein family/group databases
MEROPS	C44.001.
Enzyme and pathway databases
BRENDA	2.4.2.14. 1020.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_RHIET

Accession

Primary (citable) accession number: P77935

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)