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P77935

- PUR1_RHIEC

UniProt

P77935 - PUR1_RHIEC

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Protein
Amidophosphoribosyltransferase
Gene
purF, RHE_CH01428
Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei22 – 221Nucleophile By similarity
Metal bindingi371 – 3711Magnesium By similarity
Metal bindingi372 – 3721Magnesium By similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-1437-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPATase
Gene namesi
Name:purF
Ordered Locus Names:RHE_CH01428
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000001936: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2121 By similarity
PRO_0000029261Add
BLAST
Chaini22 – 496475Amidophosphoribosyltransferase
PRO_0000029262Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi347834.RHE_CH01428.

Structurei

3D structure databases

ProteinModelPortaliP77935.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 241220Glutamine amidotransferase type-2
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiWLVASES.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77935-1 [UniParc]FASTAAdd to Basket

« Hide

MNQSHSFPTD DPLDGDTLHE ECGVFGILGH PDAAALTALG LHALQHRGQE    50
AAGIVSFDGK RFYQERHMGL VGDHYTNPMT LARLPGSISI GHTRYSTTGE 100
VAMRNVQPLF AELEEGGIAI AHNGNFTNGL TLRRQIIATG AICQSTSDTE 150
VVLHLIARSR HASTSDRFID AIRQMEGGYS MLAMTRTKLI AARDPTGIRP 200
LVMGELDGKP IFCSETCALD IIGAKFIRDV ENGEVIICEI QPDGSISIDA 250
RKPSKPQPER LCLFEYVYFA RPDSVVGGRN VYTTRKNMGM NLAKESPVDA 300
DVVVPVPDGG TPAALGYAQE SGIPFEYGII RNHYVGRTFI EPTQQIRAFG 350
VKLKHSANRA MIEGKRVVLV DDSIVRGTTS LKIVQMIREA GAREVHIRVA 400
SPMIFFPDFY GIDTPDADKL LANQYADVEA MAKYIGADSL AFLSINGLYR 450
AVGGEDRNPA RPQFTDHYFT GDYPTRLLDK NGESMGNKLS MLASNG 496
Length:496
Mass (Da):54,162
Last modified:September 21, 2011 - v2
Checksum:iEA8F1F6F9189915C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti193 – 1942RD → SH in AAB06461. 1 Publication
Sequence conflicti312 – 3121P → S in AAB06461. 1 Publication
Sequence conflicti315 – 3151L → G in AAB06461. 1 Publication
Sequence conflicti327 – 3271Y → YEY in AAB06461. 1 Publication
Sequence conflicti407 – 4071P → R in AAB06461. 1 Publication
Sequence conflicti412 – 4176IDTPDA → SIRPTP in AAB06461. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U65392 Genomic DNA. Translation: AAB06461.1.
CP000133 Genomic DNA. Translation: ABC90231.1.
RefSeqiYP_468958.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC90231; ABC90231; RHE_CH01428.
GeneIDi3892667.
KEGGiret:RHE_CH01428.
PATRICi23084261. VBIRhiEtl108884_1802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U65392 Genomic DNA. Translation: AAB06461.1 .
CP000133 Genomic DNA. Translation: ABC90231.1 .
RefSeqi YP_468958.1. NC_007761.1.

3D structure databases

ProteinModelPortali P77935.
ModBasei Search...

Protein-protein interaction databases

STRINGi 347834.RHE_CH01428.

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC90231 ; ABC90231 ; RHE_CH01428 .
GeneIDi 3892667.
KEGGi ret:RHE_CH01428.
PATRICi 23084261. VBIRhiEtl108884_1802.

Phylogenomic databases

eggNOGi COG0034.
HOGENOMi HOG000033688.
KOi K00764.
OMAi WLVASES.
OrthoDBi EOG6KT2Q1.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci RETL347834:GJJ0-1437-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Soberon M., Lopez O., Girard L., Miranda J., Morera C.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CE3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.

Entry informationi

Entry nameiPUR1_RHIEC
AccessioniPrimary (citable) accession number: P77935
Secondary accession number(s): Q2KAA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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