P77935 (PUR1_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase
Short name=ATase
EC=2.4.2.14

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase

Gene names

Name:	purF
Ordered Locus Names:	RHE_CH01428

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium etli

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 21	21	By similarity		PRO_0000029261
	Chain	22 – 496	475	Amidophosphoribosyltransferase		PRO_0000029262
Regions
	Domain	22 – 241	220	Glutamine amidotransferase type-2
Sites
	Active site	22	1	Nucleophile By similarity
	Metal binding	371	1	Magnesium By similarity
	Metal binding	372	1	Magnesium By similarity
Experimental info
	Sequence conflict	193 – 194	2	RD → SH in AAB06461. Ref.1
	Sequence conflict	312	1	P → S in AAB06461. Ref.1
	Sequence conflict	315	1	L → G in AAB06461. Ref.1
	Sequence conflict	327	1	Y → YEY in AAB06461. Ref.1
	Sequence conflict	407	1	P → R in AAB06461. Ref.1
	Sequence conflict	412 – 417	6	IDTPDA → SIRPTP in AAB06461. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P77935 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: EA8F1F6F9189915C	FASTA	496	54,162
	10 20 30 40 50 60 MNQSHSFPTD DPLDGDTLHE ECGVFGILGH PDAAALTALG LHALQHRGQE AAGIVSFDGK 70 80 90 100 110 120 RFYQERHMGL VGDHYTNPMT LARLPGSISI GHTRYSTTGE VAMRNVQPLF AELEEGGIAI 130 140 150 160 170 180 AHNGNFTNGL TLRRQIIATG AICQSTSDTE VVLHLIARSR HASTSDRFID AIRQMEGGYS 190 200 210 220 230 240 MLAMTRTKLI AARDPTGIRP LVMGELDGKP IFCSETCALD IIGAKFIRDV ENGEVIICEI 250 260 270 280 290 300 QPDGSISIDA RKPSKPQPER LCLFEYVYFA RPDSVVGGRN VYTTRKNMGM NLAKESPVDA 310 320 330 340 350 360 DVVVPVPDGG TPAALGYAQE SGIPFEYGII RNHYVGRTFI EPTQQIRAFG VKLKHSANRA 370 380 390 400 410 420 MIEGKRVVLV DDSIVRGTTS LKIVQMIREA GAREVHIRVA SPMIFFPDFY GIDTPDADKL 430 440 450 460 470 480 LANQYADVEA MAKYIGADSL AFLSINGLYR AVGGEDRNPA RPQFTDHYFT GDYPTRLLDK 490 NGESMGNKLS MLASNG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Soberon M., Lopez O., Girard L., Miranda J., Morera C. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CE3.
[2]	"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons." Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G. Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U65392 Genomic DNA. Translation: AAB06461.1. CP000133 Genomic DNA. Translation: ABC90231.1.
RefSeq	YP_468958.1. NC_007761.1.
3D structure databases
ProteinModelPortal	P77935.
ModBase	Search...
Protein-protein interaction databases
STRING	347834.RHE_CH01428.
Protein family/group databases
MEROPS	C44.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC90231; ABC90231; RHE_CH01428.
GeneID	3892667.
KEGG	ret:RHE_CH01428.
PATRIC	23084261. VBIRhiEtl108884_1802.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0034.
HOGENOM	HOG000033688.
KO	K00764.
OMA	GIPFELG.
ProtClustDB	PRK09123.
Enzyme and pathway databases
BioCyc	RETL347834:GJJ0-1437-MONOMER.
UniPathway	UPA00074; UER00124.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR017932. GATase_2_dom. IPR000583. GATase_dom. IPR000836. PRibTrfase_dom. [Graphical view]
Pfam	PF13537. GATase_7. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_RHIEC

Accession

Primary (citable) accession number: P77935
Secondary accession number(s): Q2KAA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 21, 2011
Last modified:	April 3, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program