UniProtKB - P77935 (PUR1_RHIEC)
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Protein
Amidophosphoribosyltransferase
Gene
purF
Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Functioni
Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation
Catalytic activityi
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Amidophosphoribosyltransferase (purF)
- Phosphoribosylamine--glycine ligase (purD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 22 | NucleophileUniRule annotation | 1 |
GO - Molecular functioni
- amidophosphoribosyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
- glutamine metabolic process Source: UniProtKB-KW
- nucleoside metabolic process Source: InterPro
- purine nucleobase biosynthetic process Source: InterPro
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00074; UER00124 |
Protein family/group databases
MEROPSi | C44.001 |
Names & Taxonomyi
Protein namesi | Recommended name: AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)Short name: ATaseUniRule annotation Alternative name(s): Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation Short name: GPATaseUniRule annotation |
Gene namesi | Name:purFUniRule annotation Ordered Locus Names:RHE_CH01428 |
Organismi | Rhizobium etli (strain CFN 42 / ATCC 51251) |
Taxonomic identifieri | 347834 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000029261 | 1 – 21 | By similarityAdd BLAST | 21 | |
ChainiPRO_0000029262 | 22 – 496 | AmidophosphoribosyltransferaseAdd BLAST | 475 |
Structurei
3D structure databases
ProteinModelPortali | P77935 |
SMRi | P77935 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 241 | Glutamine amidotransferase type-2UniRule annotationAdd BLAST | 220 |
Sequence similaritiesi
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | ENOG4105CBA Bacteria COG0034 LUCA |
HOGENOMi | HOG000033688 |
KOi | K00764 |
OMAi | FRPLCLG |
Family and domain databases
CDDi | cd00715 GPATase_N, 1 hit cd06223 PRTases_typeI, 1 hit |
Gene3Di | 3.60.20.103 hits |
HAMAPi | MF_01931 PurF, 1 hit |
InterProi | View protein in InterPro IPR017932 GATase_2_dom IPR029055 Ntn_hydrolases_N IPR000836 PRibTrfase_dom IPR029057 PRTase-like IPR005854 PurF IPR035584 PurF_N |
Pfami | View protein in Pfam PF13537 GATase_7, 1 hit PF00156 Pribosyltran, 1 hit |
PIRSFi | PIRSF000485 Amd_phspho_trans, 1 hit |
SUPFAMi | SSF53271 SSF53271, 1 hit SSF56235 SSF56235, 1 hit |
TIGRFAMsi | TIGR01134 purF, 1 hit |
PROSITEi | View protein in PROSITE PS51278 GATASE_TYPE_2, 1 hit PS00103 PUR_PYR_PR_TRANSFER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P77935-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNQSHSFPTD DPLDGDTLHE ECGVFGILGH PDAAALTALG LHALQHRGQE
60 70 80 90 100
AAGIVSFDGK RFYQERHMGL VGDHYTNPMT LARLPGSISI GHTRYSTTGE
110 120 130 140 150
VAMRNVQPLF AELEEGGIAI AHNGNFTNGL TLRRQIIATG AICQSTSDTE
160 170 180 190 200
VVLHLIARSR HASTSDRFID AIRQMEGGYS MLAMTRTKLI AARDPTGIRP
210 220 230 240 250
LVMGELDGKP IFCSETCALD IIGAKFIRDV ENGEVIICEI QPDGSISIDA
260 270 280 290 300
RKPSKPQPER LCLFEYVYFA RPDSVVGGRN VYTTRKNMGM NLAKESPVDA
310 320 330 340 350
DVVVPVPDGG TPAALGYAQE SGIPFEYGII RNHYVGRTFI EPTQQIRAFG
360 370 380 390 400
VKLKHSANRA MIEGKRVVLV DDSIVRGTTS LKIVQMIREA GAREVHIRVA
410 420 430 440 450
SPMIFFPDFY GIDTPDADKL LANQYADVEA MAKYIGADSL AFLSINGLYR
460 470 480 490
AVGGEDRNPA RPQFTDHYFT GDYPTRLLDK NGESMGNKLS MLASNG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 193 – 194 | RD → SH in AAB06461 (Ref. 1) Curated | 2 | |
Sequence conflicti | 312 | P → S in AAB06461 (Ref. 1) Curated | 1 | |
Sequence conflicti | 315 | L → G in AAB06461 (Ref. 1) Curated | 1 | |
Sequence conflicti | 327 | Y → YEY in AAB06461 (Ref. 1) Curated | 1 | |
Sequence conflicti | 407 | P → R in AAB06461 (Ref. 1) Curated | 1 | |
Sequence conflicti | 412 – 417 | IDTPDA → SIRPTP in AAB06461 (Ref. 1) Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U65392 Genomic DNA Translation: AAB06461.1 CP000133 Genomic DNA Translation: ABC90231.1 |
RefSeqi | WP_011424763.1, NC_007761.1 |
Genome annotation databases
EnsemblBacteriai | ABC90231; ABC90231; RHE_CH01428 |
GeneIDi | 24299557 |
KEGGi | ret:RHE_CH01428 |
Similar proteinsi
Entry informationi
Entry namei | PUR1_RHIEC | |
Accessioni | P77935Primary (citable) accession number: P77935 Secondary accession number(s): Q2KAA5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | September 21, 2011 | |
Last modified: | March 28, 2018 | |
This is version 106 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |