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P77935

- PUR1_RHIEC

UniProt

P77935 - PUR1_RHIEC

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei22 – 221NucleophilePROSITE-ProRule annotation
    Metal bindingi371 – 3711MagnesiumBy similarity
    Metal bindingi372 – 3721MagnesiumBy similarity

    GO - Molecular functioni

    1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciRETL347834:GJJ0-1437-MONOMER.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:RHE_CH01428
    OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
    Taxonomic identifieri347834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
    ProteomesiUP000001936: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 2121By similarityPRO_0000029261Add
    BLAST
    Chaini22 – 496475AmidophosphoribosyltransferasePRO_0000029262Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi347834.RHE_CH01428.

    Structurei

    3D structure databases

    ProteinModelPortaliP77935.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 241220Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033688.
    KOiK00764.
    OMAiWLVASES.
    OrthoDBiEOG6KT2Q1.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P77935-1 [UniParc]FASTAAdd to Basket

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    MNQSHSFPTD DPLDGDTLHE ECGVFGILGH PDAAALTALG LHALQHRGQE    50
    AAGIVSFDGK RFYQERHMGL VGDHYTNPMT LARLPGSISI GHTRYSTTGE 100
    VAMRNVQPLF AELEEGGIAI AHNGNFTNGL TLRRQIIATG AICQSTSDTE 150
    VVLHLIARSR HASTSDRFID AIRQMEGGYS MLAMTRTKLI AARDPTGIRP 200
    LVMGELDGKP IFCSETCALD IIGAKFIRDV ENGEVIICEI QPDGSISIDA 250
    RKPSKPQPER LCLFEYVYFA RPDSVVGGRN VYTTRKNMGM NLAKESPVDA 300
    DVVVPVPDGG TPAALGYAQE SGIPFEYGII RNHYVGRTFI EPTQQIRAFG 350
    VKLKHSANRA MIEGKRVVLV DDSIVRGTTS LKIVQMIREA GAREVHIRVA 400
    SPMIFFPDFY GIDTPDADKL LANQYADVEA MAKYIGADSL AFLSINGLYR 450
    AVGGEDRNPA RPQFTDHYFT GDYPTRLLDK NGESMGNKLS MLASNG 496
    Length:496
    Mass (Da):54,162
    Last modified:September 21, 2011 - v2
    Checksum:iEA8F1F6F9189915C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti193 – 1942RD → SH in AAB06461. 1 PublicationCurated
    Sequence conflicti312 – 3121P → S in AAB06461. 1 PublicationCurated
    Sequence conflicti315 – 3151L → G in AAB06461. 1 PublicationCurated
    Sequence conflicti327 – 3271Y → YEY in AAB06461. 1 PublicationCurated
    Sequence conflicti407 – 4071P → R in AAB06461. 1 PublicationCurated
    Sequence conflicti412 – 4176IDTPDA → SIRPTP in AAB06461. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65392 Genomic DNA. Translation: AAB06461.1.
    CP000133 Genomic DNA. Translation: ABC90231.1.
    RefSeqiYP_468958.1. NC_007761.1.

    Genome annotation databases

    EnsemblBacteriaiABC90231; ABC90231; RHE_CH01428.
    GeneIDi3892667.
    KEGGiret:RHE_CH01428.
    PATRICi23084261. VBIRhiEtl108884_1802.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65392 Genomic DNA. Translation: AAB06461.1 .
    CP000133 Genomic DNA. Translation: ABC90231.1 .
    RefSeqi YP_468958.1. NC_007761.1.

    3D structure databases

    ProteinModelPortali P77935.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 347834.RHE_CH01428.

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC90231 ; ABC90231 ; RHE_CH01428 .
    GeneIDi 3892667.
    KEGGi ret:RHE_CH01428.
    PATRICi 23084261. VBIRhiEtl108884_1802.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033688.
    KOi K00764.
    OMAi WLVASES.
    OrthoDBi EOG6KT2Q1.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci RETL347834:GJJ0-1437-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Soberon M., Lopez O., Girard L., Miranda J., Morera C.
      Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CE3.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CFN 42 / ATCC 51251.

    Entry informationi

    Entry nameiPUR1_RHIEC
    AccessioniPrimary (citable) accession number: P77935
    Secondary accession number(s): Q2KAA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3