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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei22 – 221NucleophileUniRule annotation

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-1437-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:RHE_CH01428
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000001936: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2121By similarityPRO_0000029261Add
BLAST
Chaini22 – 496475AmidophosphoribosyltransferasePRO_0000029262Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi347834.RHE_CH01428.

Structurei

3D structure databases

ProteinModelPortaliP77935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 241220Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQSHSFPTD DPLDGDTLHE ECGVFGILGH PDAAALTALG LHALQHRGQE
60 70 80 90 100
AAGIVSFDGK RFYQERHMGL VGDHYTNPMT LARLPGSISI GHTRYSTTGE
110 120 130 140 150
VAMRNVQPLF AELEEGGIAI AHNGNFTNGL TLRRQIIATG AICQSTSDTE
160 170 180 190 200
VVLHLIARSR HASTSDRFID AIRQMEGGYS MLAMTRTKLI AARDPTGIRP
210 220 230 240 250
LVMGELDGKP IFCSETCALD IIGAKFIRDV ENGEVIICEI QPDGSISIDA
260 270 280 290 300
RKPSKPQPER LCLFEYVYFA RPDSVVGGRN VYTTRKNMGM NLAKESPVDA
310 320 330 340 350
DVVVPVPDGG TPAALGYAQE SGIPFEYGII RNHYVGRTFI EPTQQIRAFG
360 370 380 390 400
VKLKHSANRA MIEGKRVVLV DDSIVRGTTS LKIVQMIREA GAREVHIRVA
410 420 430 440 450
SPMIFFPDFY GIDTPDADKL LANQYADVEA MAKYIGADSL AFLSINGLYR
460 470 480 490
AVGGEDRNPA RPQFTDHYFT GDYPTRLLDK NGESMGNKLS MLASNG
Length:496
Mass (Da):54,162
Last modified:September 21, 2011 - v2
Checksum:iEA8F1F6F9189915C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti193 – 1942RD → SH in AAB06461 (Ref. 1) Curated
Sequence conflicti312 – 3121P → S in AAB06461 (Ref. 1) Curated
Sequence conflicti315 – 3151L → G in AAB06461 (Ref. 1) Curated
Sequence conflicti327 – 3271Y → YEY in AAB06461 (Ref. 1) Curated
Sequence conflicti407 – 4071P → R in AAB06461 (Ref. 1) Curated
Sequence conflicti412 – 4176IDTPDA → SIRPTP in AAB06461 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65392 Genomic DNA. Translation: AAB06461.1.
CP000133 Genomic DNA. Translation: ABC90231.1.
RefSeqiYP_468958.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC90231; ABC90231; RHE_CH01428.
GeneIDi3892667.
KEGGiret:RHE_CH01428.
PATRICi23084261. VBIRhiEtl108884_1802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65392 Genomic DNA. Translation: AAB06461.1.
CP000133 Genomic DNA. Translation: ABC90231.1.
RefSeqiYP_468958.1. NC_007761.1.

3D structure databases

ProteinModelPortaliP77935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347834.RHE_CH01428.

Protein family/group databases

MEROPSiC44.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC90231; ABC90231; RHE_CH01428.
GeneIDi3892667.
KEGGiret:RHE_CH01428.
PATRICi23084261. VBIRhiEtl108884_1802.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciRETL347834:GJJ0-1437-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Soberon M., Lopez O., Girard L., Miranda J., Morera C.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CE3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.

Entry informationi

Entry nameiPUR1_RHIEC
AccessioniPrimary (citable) accession number: P77935
Secondary accession number(s): Q2KAA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 21, 2011
Last modified: March 4, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.