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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei22NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:RHE_CH01428
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000001936 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000292611 – 21By similarityAdd BLAST21
ChainiPRO_000002926222 – 496AmidophosphoribosyltransferaseAdd BLAST475

Interactioni

Protein-protein interaction databases

STRINGi347834.RHE_CH01428

Structurei

3D structure databases

ProteinModelPortaliP77935
SMRiP77935
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 241Glutamine amidotransferase type-2UniRule annotationAdd BLAST220

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBA Bacteria
COG0034 LUCA
HOGENOMiHOG000033688
KOiK00764
OMAiFRPLCLG

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.103 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF13537 GATase_7, 1 hit
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQSHSFPTD DPLDGDTLHE ECGVFGILGH PDAAALTALG LHALQHRGQE
60 70 80 90 100
AAGIVSFDGK RFYQERHMGL VGDHYTNPMT LARLPGSISI GHTRYSTTGE
110 120 130 140 150
VAMRNVQPLF AELEEGGIAI AHNGNFTNGL TLRRQIIATG AICQSTSDTE
160 170 180 190 200
VVLHLIARSR HASTSDRFID AIRQMEGGYS MLAMTRTKLI AARDPTGIRP
210 220 230 240 250
LVMGELDGKP IFCSETCALD IIGAKFIRDV ENGEVIICEI QPDGSISIDA
260 270 280 290 300
RKPSKPQPER LCLFEYVYFA RPDSVVGGRN VYTTRKNMGM NLAKESPVDA
310 320 330 340 350
DVVVPVPDGG TPAALGYAQE SGIPFEYGII RNHYVGRTFI EPTQQIRAFG
360 370 380 390 400
VKLKHSANRA MIEGKRVVLV DDSIVRGTTS LKIVQMIREA GAREVHIRVA
410 420 430 440 450
SPMIFFPDFY GIDTPDADKL LANQYADVEA MAKYIGADSL AFLSINGLYR
460 470 480 490
AVGGEDRNPA RPQFTDHYFT GDYPTRLLDK NGESMGNKLS MLASNG
Length:496
Mass (Da):54,162
Last modified:September 21, 2011 - v2
Checksum:iEA8F1F6F9189915C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193 – 194RD → SH in AAB06461 (Ref. 1) Curated2
Sequence conflicti312P → S in AAB06461 (Ref. 1) Curated1
Sequence conflicti315L → G in AAB06461 (Ref. 1) Curated1
Sequence conflicti327Y → YEY in AAB06461 (Ref. 1) Curated1
Sequence conflicti407P → R in AAB06461 (Ref. 1) Curated1
Sequence conflicti412 – 417IDTPDA → SIRPTP in AAB06461 (Ref. 1) Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65392 Genomic DNA Translation: AAB06461.1
CP000133 Genomic DNA Translation: ABC90231.1
RefSeqiWP_011424763.1, NC_007761.1

Genome annotation databases

EnsemblBacteriaiABC90231; ABC90231; RHE_CH01428
GeneIDi24299557
KEGGiret:RHE_CH01428

Similar proteinsi

Entry informationi

Entry nameiPUR1_RHIEC
AccessioniPrimary (citable) accession number: P77935
Secondary accession number(s): Q2KAA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 21, 2011
Last modified: March 28, 2018
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome