P77923 (HEM2_PROFF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase
Short name=ALAD
Short name=ALADH
EC=4.2.1.24

Alternative name(s):
Porphobilinogen synthase

Gene names

Name:

hemB

Organism

Propionibacterium freudenreichii subsp. freudenreichii

Taxonomic identifier

66712 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Propionibacteriaceae › Propionibacterium › Propionibacterium freudenreichii

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.
Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Delta-aminolevulinic acid dehydratase		PRO_0000140506
Sites
	Active site	201	1	Schiff-base intermediate with substrate By similarity
	Active site	253	1	Schiff-base intermediate with substrate By similarity
	Metal binding	238	1	Magnesium By similarity
	Binding site	211	1	Substrate 1 By similarity
	Binding site	222	1	Substrate 1 By similarity
	Binding site	279	1	Substrate 2 By similarity
	Binding site	318	1	Substrate 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77923 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 09FDDC7E45D216CB	FASTA	332	35,832
	10 20 30 40 50 60 MADISIDPIV QRPRRLRRTP AIRRMVAETR LDPADLVLPM FAVEGLTEPR EIATMPGVWQ 70 80 90 100 110 120 HTKESLQKAV HDAADAGVGA VMLFGTPLER DPIGSQAWNP HGILANAVRW AVEAEPELPV 130 140 150 160 170 180 IADVCLDEFT DHGHCGVLAP DGTVDNDATL PLYAKMSVVL ADAGAAMLGP SGMMDGQIAV 190 200 210 220 230 240 IRKALDDAGH TDTVLMAYSA KYASGFFGPF REAVDSQLKG DRRAYQQDPA NAIESLREIE 250 260 270 280 290 300 LDLEQGADFV MVKPAMAYLD VLAAARAISN VPVAAYVVSG EYSMIEFAAK AGALDRKRCI 310 320 330 MEALTSVKRA GASTIVTYWA TEVAIWLNEV RS

References

[1]	Roessner C.A. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of the hemB gene encoding delta-aminolevulinic acid dehydratase from Propionibacterium freudenreichii." Hashimoto Y., Yamashita Y., Ono H., Murooka Y. J. Ferment. Bioeng. 82:93-100(1996) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6207 / DSM 20271 / LMG 16412 / NBRC 12424 / NCIMB 5959 / NCTC 10470 / NRRL B-3523.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51164 Genomic DNA. Translation: AAB07863.1. D85417 Genomic DNA. Translation: BAA21911.1.
3D structure databases
ProteinModelPortal	P77923.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00251; UER00318.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
PANTHER	PTHR11458. PTHR11458. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_PROFF

Accession

Primary (citable) accession number: P77923

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	March 6, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program