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P77923

- HEM2_PROFF

UniProt

P77923 - HEM2_PROFF

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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Propionibacterium freudenreichii subsp. freudenreichii
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei201 – 2011Schiff-base intermediate with substrateBy similarity
Binding sitei211 – 2111Substrate 1By similarity
Binding sitei222 – 2221Substrate 1By similarity
Metal bindingi238 – 2381MagnesiumBy similarity
Active sitei253 – 2531Schiff-base intermediate with substrateBy similarity
Binding sitei279 – 2791Substrate 2By similarity
Binding sitei318 – 3181Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
OrganismiPropionibacterium freudenreichii subsp. freudenreichii
Taxonomic identifieri66712 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Delta-aminolevulinic acid dehydratasePRO_0000140506Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP77923.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77923-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADISIDPIV QRPRRLRRTP AIRRMVAETR LDPADLVLPM FAVEGLTEPR
60 70 80 90 100
EIATMPGVWQ HTKESLQKAV HDAADAGVGA VMLFGTPLER DPIGSQAWNP
110 120 130 140 150
HGILANAVRW AVEAEPELPV IADVCLDEFT DHGHCGVLAP DGTVDNDATL
160 170 180 190 200
PLYAKMSVVL ADAGAAMLGP SGMMDGQIAV IRKALDDAGH TDTVLMAYSA
210 220 230 240 250
KYASGFFGPF REAVDSQLKG DRRAYQQDPA NAIESLREIE LDLEQGADFV
260 270 280 290 300
MVKPAMAYLD VLAAARAISN VPVAAYVVSG EYSMIEFAAK AGALDRKRCI
310 320 330
MEALTSVKRA GASTIVTYWA TEVAIWLNEV RS
Length:332
Mass (Da):35,832
Last modified:February 1, 1997 - v1
Checksum:i09FDDC7E45D216CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51164 Genomic DNA. Translation: AAB07863.1.
D85417 Genomic DNA. Translation: BAA21911.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51164 Genomic DNA. Translation: AAB07863.1 .
D85417 Genomic DNA. Translation: BAA21911.1 .

3D structure databases

ProteinModelPortali P77923.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Roessner C.A.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of the hemB gene encoding delta-aminolevulinic acid dehydratase from Propionibacterium freudenreichii."
    Hashimoto Y., Yamashita Y., Ono H., Murooka Y.
    J. Ferment. Bioeng. 82:93-100(1996)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 6207 / DSM 20271 / LMG 16412 / NBRC 12424 / NCIMB 5959 / NCTC 10470 / NRRL B-3523.

Entry informationi

Entry nameiHEM2_PROFF
AccessioniPrimary (citable) accession number: P77923
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3