Reviewed, UniProtKB/Swiss-Prot P77918 (PYRB_PYRAB)

Names and origin

Protein names

Recommended name:
    Aspartate carbamoyltransferase
    EC=2.1.3.2
Alternative name(s):
    Aspartate transcarbamylase
      Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	PYRAB13270
ORF Names:	PAB1498

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 2/6. HAMAP MF_00001
Subunit structure	Heterooligomer of catalytic and regulatory chains. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 308

308

Aspartate carbamoyltransferase HAMAP MF_00001

PRO_0000113253

Secondary structure

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308

Helix Strand Turn

Details...

Helix

11 – 13

3

Helix

16 – 36

21

Turn

41 – 44

4

Beta strand

46 – 53

8

Helix

57 – 68

12

Beta strand

72 – 77

6

Helix

79 – 81

3

Helix

83 – 86

4

Helix

90 – 97

8

Turn

98 – 100

3

Beta strand

102 – 110

9

Helix

113 – 119

7

Beta strand

125 – 130

6

Helix

136 – 149

14

Beta strand

153 – 162

10

Turn

164 – 166

3

Helix

168 – 176

9

Helix

177 – 179

3

Beta strand

182 – 187

6

Helix

190 – 192

3

Helix

196 – 204

9

Beta strand

209 – 213

5

Helix

216 – 219

4

Beta strand

223 – 227

5

Helix

232 – 234

3

Beta strand

235 – 237

3

Helix

238 – 242

5

Turn

243 – 246

4

Helix

254 – 257

4

Beta strand

263 – 265

3

Beta strand

271 – 274

4

Helix

276 – 280

5

Helix

286 – 291

6

Helix

293 – 304

12

Sequences

	Sequence		Length	Mass (Da)
	P77918-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: D36961E7749C012E	FASTA	308	34,901
	10 20 30 40 50 60 MDWKGRDVIS IRDFSKEDIE TVLATAERLE RELKEKGQLE YAKGKILATL FFEPSTRTRL 70 80 90 100 110 120 SFESAMHRLG GAVIGFAEAS TSSVKKGESL RDTIKTVEQY CDVIVIRHPK EGAARLAAEV 130 140 150 160 170 180 AEVPVINAGD GSNQHPTQTL LDLYTIKKEF GRIDGLKIGL LGDLKYGRTV HSLAEALTFY 190 200 210 220 230 240 DVELYLISPE LLRMPRHIVE ELREKGMKVV ETTTLEDVIG KLDVLYVTRI QKERFPDEQE 250 260 270 280 290 300 YLKVKGSYQV NLKVLEKAKD ELRIMHPLPR VDEIHPEVDN TKHAIYFRQV FNGVPVRMAL LALVLGVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Aspartate transcarbamylase from the deep-sea hyperthermophilic archaeon Pyrococcus abyssi: genetic organization, structure, and expression in Escherichia coli." Purcarea C., Herve G., Ladjimi M.M., Cunin R. J. Bacteriol. 179:4143-4157(1997) [PubMed: 9209027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GE5 / Orsay.
[2]	"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U61765 Genomic DNA. Translation: AAB62984.1.
AJ248287 Genomic DNA. Translation: CAB50232.1.

PIR

C75042.

RefSeq

NP_127002.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ML4	X-ray	1.80	A	1-308	[»]

ModBase

Search...

Genome annotation databases

GeneID

1496715.

GenomeReviews

Gene locus PYRAB13270 in contig AL096836_GR.

KEGG

pab:PAB1498.

NMPDR

fig|272844.1.peg.1408.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P77918.

OMA

P77918. RFDESEY.

Enzyme and pathway databases

BioCyc

PABY272844:PAB1498-MON.

BRENDA

2.1.3.2. 262861.

Family and domain databases

HAMAP

MF_00001.
[Tree]

InterPro

IPR006132. Asp/Orn_carbamoyltranf_P_bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn_bd.
IPR002082. Aspartate_carbamoyltransf_euk.
[Graphical view]

Pfam

PF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]

PRINTS

PR00100. AOTCASE.
PR00101. ATCASE.

TIGRFAMs

TIGR00670. asp_carb_tr. 1 hit.

PROSITE

PS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PYRB_PYRAB

Accession

Primary (citable) accession number: P77918

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)