Oxygen-independent coproporphyrinogen III oxidase

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Reviewed, UniProtKB/Swiss-Prot P77915 (HEMN_PSEAE)

Last modified November 25, 2008. Version 61. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemN
Ordered Locus Names:	PA1546

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO(2) + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen III oxidase family.
Sequence caution	The sequence AAA25710.1 differs from that shown. Reason: Frameshift at position 160. The sequence AAA25711.1 differs from that shown. Reason: Frameshift at position 160. The sequence CAA40903.1 differs from that shown. Reason: Frameshift at position 160. The sequence CAA40904.1 differs from that shown. Reason: Frameshift at position 160.

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Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 460

460

Oxygen-independent coproporphyrinogen III oxidase

PRO_0000109947

Regions

Region

115 – 116

S-adenosyl-L-methionine 2 binding By similarity

Sites

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Binding site

S-adenosyl-L-methionine 1 By similarity

Binding site

S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity

Binding site

114

S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity

Binding site

149

S-adenosyl-L-methionine 1 By similarity

Binding site

176

S-adenosyl-L-methionine 2 By similarity

Binding site

188

S-adenosyl-L-methionine 2 By similarity

Binding site

213

S-adenosyl-L-methionine 2 By similarity

Experimental info

Sequence conflict

104

E → A Ref.1 Ref.3

Sequence conflict

422

L → P in CAA66617. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P77915-1 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: A15EFD0FD04AB03E
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FASTA

460

52,488

        10         20         30         40         50         60 
MLDTIRWDAD LIRRYDLSGP RYTSYPTAVQ FHEGIGPFDQ LHALRDSRKA GHPLSLYVHI 

        70         80         90        100        110        120 
PFCANICYYC ACNKVITKDR GRSAPYLARL VREIEIVSRH LSREQVVEQL HFGGGTPTFL 

       130        140        150        160        170        180 
SPGQLRELMS QLRTHLNLLD DDSGDYGIEI DPREADWSTM GLLRELGFNR VSLGVQDFDM 

       190        200        210        220        230        240 
EVQKAVNRMQ TPEETRTIVE AARTLQYRSI NLDLIYGLPK QTPDSFARTV DEVIALQPDR 

       250        260        270        280        290        300 
LSVFNYAHLP ERFMPQRRIN ADDLPSPGQK LEMLQRTTEQ LAAAGYRYIG MDHFALPDDE 

       310        320        330        340        350        360 
LASAQEDGTL QRNFQGYTTH GHCDLVGLGV SAISQIGDLY SQNSSDINDY QTSLDNGQLA 

       370        380        390        400        410        420 
IRRGLHCNSD DRVRRAVIQQ LICHFELAFE DIETEFGIDF RSYFAELWPD LERFAADGLI 

       430        440        450        460 
RLDAKGIDIT SSGRLLVRSI CMLFDRYLPS LNRQRFSRVI

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr." Rompf A., Hungerer C., Hoffmann T., Lindenmeyer M., Romling U., Gross U., Doss M.O., Arai H., Igarashi Y., Jahn D. Mol. Microbiol. 29:985-997(1998) [PubMed: 9767567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"RpoN-independent promoters having a conserved GG-N10-GC motif in Pseudomonas aeruginosa." Savioz A., Zimmermann A., Haas D. Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

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Cross-references

Sequence databases
	X97981 Genomic DNA. Translation: CAA66617.1. AE004091 Genomic DNA. Translation: AAG04935.1. M98276 Genomic DNA. Translation: AAA25710.1. Frameshift. M98276 Genomic DNA. Translation: AAA25711.1. Frameshift. X57736 Genomic DNA. Translation: CAA40903.1. Frameshift. X57736 Genomic DNA. Translation: CAA40904.1. Frameshift.
PIR	A83454.
RefSeq	NP_250237.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	883053.
GenomeReviews	Gene locus PA1546 in contig AE004091_GR.
KEGG	pae:PA1546.
Organism-specific databases
PseudoCAP	PA1546.
CMR	Search...
Phylogenomic databases
HOGENOM	P77915.
Enzyme and pathway databases
BioCyc	PAER208964:PA1546-MON.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004558. HemN. IPR010723. HemN_C. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00538. hemN. 1 hit.
ProtoNet	Search...

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Entry information

Entry name

HEMN_PSEAE

Accession

Primary (citable) accession number: P77915
Secondary accession number(s): Q04626, Q04627

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	November 25, 2008
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents