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Protein

Carbamoyl-phosphate synthase pyrimidine-specific small chain

Gene

pyrAA

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Enzyme regulationi

Inhibited by pyrimidine.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA), Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB), Carbamoyl-phosphate synthase small chain (pyrAA2)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei244 – 2441NucleophileBy similarity
Active sitei329 – 3291By similarity
Active sitei331 – 3311By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLPLA220668-WGS:GSPK-2313-MONOMER.
UniPathwayiUPA00070; UER00115.

Protein family/group databases

MEROPSiC26.963.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase pyrimidine-specific small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Short name:
CPS-P
Gene namesi
Name:pyrAA
Ordered Locus Names:lp_2701
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000000432 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Carbamoyl-phosphate synthase pyrimidine-specific small chainPRO_0000112286Add
BLAST

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Protein-protein interaction databases

STRINGi220668.lp_2701.

Structurei

3D structure databases

ProteinModelPortaliP77885.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini169 – 356188Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 165165CPSaseAdd
BLAST

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
HOGENOMiHOG000038087.
KOiK01956.
OMAiTNAHIGN.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRYLVLEDG TIYPGTGFGA TTATVGELVF NTGMSGYQES ITDQSYNGEI
60 70 80 90 100
LMFTYPLIGN YGINRDDHES IKPTCKGVVV HEVARRASNW RNAQSLDDYL
110 120 130 140 150
KQNAIPGIMD IDTRAVTKHI RTKGAMKATI VDNVLPDTVD RLKVTELNRA
160 170 180 190 200
VVAQSSTNNA YPNPATGPNV VVVDFGLKHS ILRELAKRQC NLTVLPYNTT
210 220 230 240 250
ASEIMALNPD GVMLTNGPGD PKDVPGALEM IREVEKHVPL FGICLGHQLF
260 270 280 290 300
ALANGADTFK MKFGHRGFNH PVREIATGRI DFTSQNHGYA VDRDSLAQTD
310 320 330 340 350
LLITHEEIND GTVEGLRHRD YAAFSVQYHP DAAPGPHDAD HIFDEFIDLM
360
AANQATQKGS QFNA
Length:364
Mass (Da):39,983
Last modified:March 25, 2003 - v2
Checksum:i5B916580EE4C3267
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441V → I in CAA91004 (PubMed:8982065).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54240 Genomic DNA. Translation: CAA91004.1.
AL935263 Genomic DNA. Translation: CCC79822.1.
RefSeqiWP_011101887.1. NC_004567.2.
YP_004890336.1. NC_004567.2.

Genome annotation databases

EnsemblBacteriaiCCC79822; CCC79822; lp_2701.
GeneIDi1062973.
KEGGilpl:lp_2701.
PATRICi22251307. VBILacPla27411_2261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54240 Genomic DNA. Translation: CAA91004.1.
AL935263 Genomic DNA. Translation: CCC79822.1.
RefSeqiWP_011101887.1. NC_004567.2.
YP_004890336.1. NC_004567.2.

3D structure databases

ProteinModelPortaliP77885.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220668.lp_2701.

Protein family/group databases

MEROPSiC26.963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC79822; CCC79822; lp_2701.
GeneIDi1062973.
KEGGilpl:lp_2701.
PATRICi22251307. VBILacPla27411_2261.

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
HOGENOMiHOG000038087.
KOiK01956.
OMAiTNAHIGN.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00115.
BioCyciLPLA220668-WGS:GSPK-2313-MONOMER.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and organisation of the pyrimidine biosynthesis pathway genes in Lactobacillus plantarum: a PCR strategy for sequencing without cloning."
    Elagoez A., Abdi A., Hubert J.-C., Kammerer B.
    Gene 182:37-43(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 8014 / CCM 1904 / DSM 20205 / NCDO 82 / NCIB 6376.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.
  3. "In Lactobacillus plantarum, carbamoyl phosphate is synthesized by two carbamoyl-phosphate synthetases (CPS): carbon dioxide differentiates the arginine-repressed from the pyrimidine-regulated CPS."
    Nicoloff H., Hubert J.-C., Bringel F.
    J. Bacteriol. 182:3416-3422(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: ATCC 8014 / CCM 1904 / DSM 20205 / NCDO 82 / NCIB 6376.

Entry informationi

Entry nameiCARA_LACPL
AccessioniPrimary (citable) accession number: P77885
Secondary accession number(s): F9URI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: March 25, 2003
Last modified: November 11, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.