Reviewed, UniProtKB/Swiss-Prot P77885 (CARA_LACPL)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase pyrimidine-specific small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain
      Short name=CPS-P

Gene names

Name:	pyrAA
Ordered Locus Names:	lp_2701

Organism

Lactobacillus plantarum [Complete proteome] [HAMAP]

Taxonomic identifier

1590 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Enzyme regulation	Inhibited by pyrimidine. HAMAP MF_01209
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Carbamoyl-phosphate synthase pyrimidine-specific small chain HAMAP MF_01209		PRO_0000112286
Regions
	Domain	169 – 356	188	Glutamine amidotransferase type-1
	Region	1 – 165	165	CPSase HAMAP MF_01209
Sites
	Active site	244	1	Nucleophile By similarity
	Active site	329	1	By similarity
	Active site	331	1	By similarity
Experimental info
	Sequence conflict	144	1	V → I in CAA91004. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P77885-1 [UniParc]. Last modified March 25, 2003. Version 2. Checksum: 5B916580EE4C3267	FASTA	364	39,983
	10 20 30 40 50 60 MKRYLVLEDG TIYPGTGFGA TTATVGELVF NTGMSGYQES ITDQSYNGEI LMFTYPLIGN 70 80 90 100 110 120 YGINRDDHES IKPTCKGVVV HEVARRASNW RNAQSLDDYL KQNAIPGIMD IDTRAVTKHI 130 140 150 160 170 180 RTKGAMKATI VDNVLPDTVD RLKVTELNRA VVAQSSTNNA YPNPATGPNV VVVDFGLKHS 190 200 210 220 230 240 ILRELAKRQC NLTVLPYNTT ASEIMALNPD GVMLTNGPGD PKDVPGALEM IREVEKHVPL 250 260 270 280 290 300 FGICLGHQLF ALANGADTFK MKFGHRGFNH PVREIATGRI DFTSQNHGYA VDRDSLAQTD 310 320 330 340 350 360 LLITHEEIND GTVEGLRHRD YAAFSVQYHP DAAPGPHDAD HIFDEFIDLM AANQATQKGS QFNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organisation of the pyrimidine biosynthesis pathway genes in Lactobacillus plantarum: a PCR strategy for sequencing without cloning." Elagoez A., Abdi A., Hubert J.-C., Kammerer B. Gene 182:37-43(1996) [PubMed: 8982065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 8014 / CCM 1904 / DSM 20205 / NCDO 82 / NCIB 6376.
[2]	"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.
[3]	"In Lactobacillus plantarum, carbamoyl phosphate is synthesized by two carbamoyl-phosphate synthetases (CPS): carbon dioxide differentiates the arginine-repressed from the pyrimidine-regulated CPS." Nicoloff H., Hubert J.-C., Bringel F. J. Bacteriol. 182:3416-3422(2000) [PubMed: 10852872] [Abstract] Cited for: FUNCTION. Strain: ATCC 8014 / CCM 1904 / DSM 20205 / NCDO 82 / NCIB 6376.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z54240 Genomic DNA. Translation: CAA91004.1. AL935259 Genomic DNA. Translation: CAD64944.1.
RefSeq	NP_786093.1.
3D structure databases
HSSP	HSSP built from PDB template 1A9X based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1062973.
GenomeReviews	Gene locus lp_2701 in contig AL935263_GR.
KEGG	lpl:lp_2701.
NMPDR	fig|220668.1.peg.2227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P77885.
OMA	P77885. LFDGSNC.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_2701-MON.
BRENDA	6.3.5.5. 593.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_LACPL

Accession

Primary (citable) accession number: P77885

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	March 25, 2003
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)