P77883 (PYRB_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	lp_2703

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000113148

Sequences

	Sequence		Length	Mass (Da)
	P77883 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C035D9D9281A5431	FASTA	311	34,712
	10 20 30 40 50 60 MKTSQNLVSV EQFSNQDVMA YLKLAQAFKN GKTVQLSQPT FAMNLFFENS TRTHTSFEMA 70 80 90 100 110 120 ERRLGLQVIP FDPKTSSVTK GESLLDTLKT IEAIGVNLAV VRHPRDRYYQ PLLDAGFDMS 130 140 150 160 170 180 LINAGDGSGQ HPSQSLLDML TIYEEFGHFD GLKIAIVGDL AHSRVARSNM ALLTQLGAQV 190 200 210 220 230 240 YFGGPKEWYG RDFEAYGEYQ TMDQLVATMD VMMLLRVQHE RLSQVNNQTF DASAYHQQYG 250 260 270 280 290 300 LTAERAARMP KHAIIMHPAP VNRGVELASD LVEAPQSRIF QQMTNGVYIR MAMVASVLAH 310 QGLISATQVE V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organisation of the pyrimidine biosynthesis pathway genes in Lactobacillus plantarum: a PCR strategy for sequencing without cloning." Elagoez A., Abdi A., Hubert J.-C., Kammerer B. Gene 182:37-43(1996) [PubMed: 8982065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 8014 / CCM 1904 / DSM 20205 / NCDO 82 / NCIB 6376.
[2]	"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z54240 Genomic DNA. Translation: CAA91002.1. AL935263 Genomic DNA. Translation: CCC79824.1.
RefSeq	NP_786095.1. NC_004567.1.
3D structure databases
ProteinModelPortal	P77883.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1062971.
GenomeReviews	Gene locus lp_2703 in contig AL935263_GR.
KEGG	lpl:lp_2703.
NMPDR	fig|220668.1.peg.2229.
PATRIC	22251311. VBILacPla27411_2263.
Phylogenomic databases
HOGENOM	HBG579429.
OMA	VGIPIIN.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_2703-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_LACPL

Accession

Primary (citable) accession number: P77883
Secondary accession number(s): F9URI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program