P77858 (HYFC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydrogenase-4 component C
EC=1.-.-.-

Gene names

Name:	hyfC
Ordered Locus Names:	b2483, JW2468

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Hydrogenase-4 component C		PRO_0000117535
Regions
	Topological domain	1 – 10	10	Periplasmic Potential
	Transmembrane	11 – 31	21	Helical; Potential
	Topological domain	32 – 73	42	Cytoplasmic Potential
	Transmembrane	74 – 94	21	Helical; Potential
	Topological domain	95 – 98	4	Periplasmic Potential
	Transmembrane	99 – 119	21	Helical; Potential
	Topological domain	120 – 140	21	Cytoplasmic Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Topological domain	162 – 181	20	Periplasmic Potential
	Transmembrane	182 – 202	21	Helical; Potential
	Topological domain	203 – 228	26	Cytoplasmic Potential
	Transmembrane	229 – 249	21	Helical; Potential
	Topological domain	250 – 256	7	Periplasmic Potential
	Transmembrane	257 – 277	21	Helical; Potential
	Topological domain	278 – 289	12	Cytoplasmic Potential
	Transmembrane	290 – 310	21	Helical; Potential
	Topological domain	311 – 315	5	Periplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P77858 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 18B2988ED37DD1C9	FASTA	315	34,359
	10 20 30 40 50 60 MRQTLCDGYL VIFALAQAVI LLMLTPLFTG ISRQIRARMH SRRGPGIWQD YRDIHKLFKR 70 80 90 100 110 120 QEVAPTSSGL MFRLMPWVLI SSMLVLAMAL PLFITVSPFA GGGDLITLIY LLALFRFFFA 130 140 150 160 170 180 LSGLDTGSPF AGVGASRELT LGILVEPMLI LSLLVLALIA GSTHIEMISN TLAMGWNSPL 190 200 210 220 230 240 TTVLALLACG FACFIEMGKI PFDVAEAEQE LQEGPLTEYS GAGLALAKWG LGLKQVVMAS 250 260 270 280 290 300 LFVALFLPFG RAQELSLACL LTSLVVTLLK VLLIFVLASI AENTLARGRF LLIHHVTWLG 310 FSLAALAWVF WLTGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63654 Genomic DNA. Translation: AAB88565.1. U00096 Genomic DNA. Translation: AAC75536.2. AP009048 Genomic DNA. Translation: BAA16361.2.
PIR	B65024.
RefSeq	NP_416978.4. NC_000913.2.
3D structure databases
ProteinModelPortal	P77858.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000270; EBESCP00000000270; EBESCG00000000224. EBESCT00000015401; EBESCP00000014692; EBESCG00000014461.
GeneID	947492.
GenomeReviews	Gene locus JW2468 in contig AP009048_GR. Gene locus b2483 in contig U00096_GR.
KEGG	ecj:JW2468. eco:b2483.
PATRIC	32120353. VBIEscCol129921_2579.
Organism-specific databases
EchoBASE	EB3963.
EcoGene	EG14211. hyfC.
Phylogenomic databases
eggNOG	COG0650.
GeneTree	EBGT00050000009143.
HOGENOM	HBG727670.
OMA	ADSTHIE.
PhylomeDB	P77858.
ProtClustDB	CLSK880406.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-154.
Gene expression databases
Genevestigator	P77858.
Family and domain databases
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
KO	K12138.
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. False negative. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYFC_ECOLI

Accession

Primary (citable) accession number: P77858
Secondary accession number(s): P78262

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program