Beta-1,4-mannanase - Caldocellum saccharolyticum

Preferred order of sections

Names and origin

Protein names

Submitted name:
Beta-1,4-mannanase EMBL AAC44232.1
EC=3.2.1.78 EMBL AAC44232.1

Gene names

Name:

manA EMBL AAC44232.1

Organism

Caldocellum saccharolyticum (Caldicellulosiruptor saccharolyticus) EMBL AAC44232.1

Taxonomic identifier

44001 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1PMJ PDB 1PMH Metal-binding PDB 1PMJ PDB 1PMH
Molecular function	Glycosidase EMBL AAC44232.1 Hydrolase
Technical term	3D-structure PDB 1PMJ PDB 1PMH
Gene Ontology (GO)
Biological process	substituted mannan metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

95 – 97

3

Beta-D-mannose binding PDB 1PMH

Region

145 – 148

4

Beta-D-mannose binding PDB 1PMH

Sites

Metal binding

45

1

Calcium; via carbonyl oxygen PDB 1PMJ PDB 1PMH

Metal binding

47

1

Calcium PDB 1PMJ PDB 1PMH

Metal binding

79

1

Calcium PDB 1PMJ PDB 1PMH

Metal binding

81

1

Calcium; via carbonyl oxygen PDB 1PMJ PDB 1PMH

Metal binding

211

1

Calcium PDB 1PMJ PDB 1PMH

Binding site

141

1

Beta-D-mannose PDB 1PMH

Binding site

196

1

Beta-D-mannose PDB 1PMH

Sequences

Sequence

Length

Mass (Da)

Tools

P77847 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: AD4A7FF0C1C8DF0B
Show »

FASTA

911

103,325

        10         20         30         40         50         60 
MGVCLDMWRN WRVLFVLVTI IFLFSLIPTG IFGAVESSVN PVVLDFEDGT VMSFGEAWGD 

        70         80         90        100        110        120 
SLKCIKKVSV SQDLQRPGNK YALRLDVEFN PNNGWDQGDL GTWFGGVVEG QFDFTGYKSV 

       130        140        150        160        170        180 
EFEMFIPYDE FSKSQGGFPY KVVINDGWKE LGSEFNITAN AGKKVKINGK DYTVIHKAFA 

       190        200        210        220        230        240 
IPEDFRTKKR AQLVFQFAGQ NSNYKGPIYL DNVRIRPEDA SNLSKEDYGE QAEEGVAVED 

       250        260        270        280        290        300 
FFTGVKLVYP EQGKSFVYNF EIDTMGFYKY SGDGFNKKTK SLEYSQDLKR SGNNGALKVN 

       310        320        330        340        350        360 
ASLAGTAFDE MNIAIKLTDK DDKKFDFSKY STLEYTLYIP NPDKISGKLM VASAIDNPWQ 

       370        380        390        400        410        420 
IIKDFTAINY KDKNAIQKIN GKDYAVIKCS DNLYNVNTKA NVLVLRIAGS YVKYTGPIYI 

       430        440        450        460        470        480 
DNVKLVAGKK VAPKVKTTSS IPNIKNYYRV KIEAETAKDG WAYSIEKENA KYSGKGYILL 

       490        500        510        520        530        540 
FGNNMGNTLY DLKIPKTGYY IFTISSSTLG MVNYGSVDIW IDGELKGAAK VPNVKGKFQE 

       550        560        570        580        590        600 
VVVMKKIYLT QGEHTLSLQK SGGYTIAIDY FTIEELVLAN KNKISVDTKL VTPNPHPNAQ 

       610        620        630        640        650        660 
KLMKYLASIY GEKILSGQQS SGDGKEIQMI FDVTKRYPAV RGFDFMDYSP SRVEHGTKGT 

       670        680        690        700        710        720 
DVEEAIKWWK SGGIVAFCWH WNAPTGLIDQ PGKEWWRGFY TEATTFDIKK AMDNPNSTEY 

       730        740        750        760        770        780 
KLILRDIDAI AEQLKRLQKE GVPVLFRPLH EASGGWFWWG AKGPEPYIKL WKLMFDRLVN 

       790        800        810        820        830        840 
YHKLNNLIWV WNGQDAAWYP GDNYVDIIGE DIYEEKAQYS PYADRFAKAL KYTNARKMIA 

       850        860        870        880        890        900 
LTECGTIPDP AVLKQEGISW SWFSVWAGNF VMTGSKYNDE WNDNHMLRKI YNSDYVITKD 

       910 
ELPDIKNIPL K

« Hide

References

[1]	"Sequencing, cloning and expression of a beta-1,4-mannanase gene, manA, from the extremely thermophilic anaerobic bacterium, Caldicellulosiruptor Rt8B.4." Gibbs M.D., Elinder A.U., Reeves R.A., Bergquist P.L. FEMS Microbiol. Lett. 141:37-43(1996) [PubMed: 8764509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Rt8B.4 EMBL AAC44232.1.
[2]	"High-resolution crystal structures of Caldicellulosiruptor strain Rt8B.4 carbohydrate-binding module CBM27-1 and its complex with mannohexaose." Roske Y., Sunna A., Pfeil W., Heinemann U. J. Mol. Biol. 340:543-554(2004) [PubMed: 15210353] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS) OF 36-220 IN COMPLEX WITH BETA-D-MANNOSE AND CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U39812 Genomic DNA. Translation: AAC44232.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PMH	X-ray	1.06	X	36-220	[»]
1PMJ	X-ray	1.55	X	36-220	[»]

ProteinModelPortal

P77847.

SMR

P77847. Positions 36-220.

ModBase

Search...

Protein family/group databases

CAZy

CBM27. Carbohydrate-Binding Module Family 27.
CBM35. Carbohydrate-Binding Module Family 35.
GH26. Glycoside Hydrolase Family 26.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR015295. Carb-bd_module_27.
IPR005084. CMB_fam6.
IPR022790. EndoGluc_H/Glyco_hydro_26.
IPR008979. Galactose-bd-like.
IPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF09212. CBM27. 2 hits.
PF03422. CBM_6. 1 hit.
PF02156. Glyco_hydro_26. 1 hit.
[Graphical view]

PRINTS

PR00739. GLHYDRLASE26.

SUPFAM

SSF49785. Gal_bind_like. 3 hits.
SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS51175. CBM6. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

P77847_CALSA

Accession

Primary (citable) accession number: P77847

Entry history

Integrated into UniProtKB/TrEMBL:	February 1, 1997
Last sequence update:	February 1, 1997
Last modified:	September 21, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)