Unreviewed, UniProtKB/TrEMBL P77847 (P77847_CALSA)

Names and origin

Protein names

Submitted name:
    Beta-1,4-mannanase EMBL AAC44232.1
    EC=3.2.1.78

Gene names

Name:

manA EMBL AAC44232.1

Organism

Caldocellum saccharolyticum (Caldicellulosiruptor saccharolyticus) EMBL AAC44232.1

Taxonomic identifier

44001 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: InterPro substituted mannan metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	P77847-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: AD4A7FF0C1C8DF0B	FASTA	911	103,325
	10 20 30 40 50 60 MGVCLDMWRN WRVLFVLVTI IFLFSLIPTG IFGAVESSVN PVVLDFEDGT VMSFGEAWGD 70 80 90 100 110 120 SLKCIKKVSV SQDLQRPGNK YALRLDVEFN PNNGWDQGDL GTWFGGVVEG QFDFTGYKSV 130 140 150 160 170 180 EFEMFIPYDE FSKSQGGFPY KVVINDGWKE LGSEFNITAN AGKKVKINGK DYTVIHKAFA 190 200 210 220 230 240 IPEDFRTKKR AQLVFQFAGQ NSNYKGPIYL DNVRIRPEDA SNLSKEDYGE QAEEGVAVED 250 260 270 280 290 300 FFTGVKLVYP EQGKSFVYNF EIDTMGFYKY SGDGFNKKTK SLEYSQDLKR SGNNGALKVN 310 320 330 340 350 360 ASLAGTAFDE MNIAIKLTDK DDKKFDFSKY STLEYTLYIP NPDKISGKLM VASAIDNPWQ 370 380 390 400 410 420 IIKDFTAINY KDKNAIQKIN GKDYAVIKCS DNLYNVNTKA NVLVLRIAGS YVKYTGPIYI 430 440 450 460 470 480 DNVKLVAGKK VAPKVKTTSS IPNIKNYYRV KIEAETAKDG WAYSIEKENA KYSGKGYILL 490 500 510 520 530 540 FGNNMGNTLY DLKIPKTGYY IFTISSSTLG MVNYGSVDIW IDGELKGAAK VPNVKGKFQE 550 560 570 580 590 600 VVVMKKIYLT QGEHTLSLQK SGGYTIAIDY FTIEELVLAN KNKISVDTKL VTPNPHPNAQ 610 620 630 640 650 660 KLMKYLASIY GEKILSGQQS SGDGKEIQMI FDVTKRYPAV RGFDFMDYSP SRVEHGTKGT 670 680 690 700 710 720 DVEEAIKWWK SGGIVAFCWH WNAPTGLIDQ PGKEWWRGFY TEATTFDIKK AMDNPNSTEY 730 740 750 760 770 780 KLILRDIDAI AEQLKRLQKE GVPVLFRPLH EASGGWFWWG AKGPEPYIKL WKLMFDRLVN 790 800 810 820 830 840 YHKLNNLIWV WNGQDAAWYP GDNYVDIIGE DIYEEKAQYS PYADRFAKAL KYTNARKMIA 850 860 870 880 890 900 LTECGTIPDP AVLKQEGISW SWFSVWAGNF VMTGSKYNDE WNDNHMLRKI YNSDYVITKD 910 ELPDIKNIPL K

References

[1]	"Sequencing, cloning and expression of a beta-1,4-mannanase gene, manA, from the extremely thermophilic anaerobic bacterium, Caldicellulosiruptor Rt8B.4." Gibbs M.D., Elinder A.U., Reeves R.A., Bergquist P.L. FEMS Microbiol. Lett. 141:37-43(1996) [PubMed: 8764509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Rt8B.4 EMBL AAC44232.1.
[2]	"High-resolution crystal structures of Caldicellulosiruptor strain Rt8B.4 carbohydrate-binding module CBM27-1 and its complex with mannohexaose." Roske Y., Sunna A., Pfeil W., Heinemann U. J. Mol. Biol. 340:543-554(2004) [PubMed: 15210353] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS) OF 36-220.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U39812 Genomic DNA. Translation: AAC44232.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PMH	X-ray	1.06	X	36-220	[»]
1PMJ	X-ray	1.55	X	36-220	[»]

ModBase

Search...

Protein family/group databases

CAZy

CBM27. Carbohydrate-Binding Module Family 27.
CBM35. Carbohydrate-Binding Module Family 35.
GH26. Glycoside Hydrolase Family 26.

Family and domain databases

InterPro

IPR005086. CBM_17_28.
IPR005084. CBM_6.
IPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF03424. CBM_17_28. 2 hits.
PF03422. CBM_6. 1 hit.
PF02156. Glyco_hydro_26. 1 hit.
[Graphical view]

PRINTS

PR00739. GLHYDRLASE26.

PROSITE

PS51175. CBM6. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

P77847_CALSA

Accession

Primary (citable) accession number: P77847

Entry history

Integrated into UniProtKB/TrEMBL:	February 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)