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Protein

Acetate kinase

Gene

ackA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8MagnesiumUniRule annotation1
Binding sitei15ATPUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Active sitei149Proton donor/acceptorUniRule annotation1
Sitei181Transition state stabilizerUniRule annotation1
Sitei242Transition state stabilizerUniRule annotation1
Metal bindingi385MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi209 – 213ATPUniRule annotation5
Nucleotide bindingi283 – 285ATPUniRule annotation3
Nucleotide bindingi331 – 335ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCORYNE:G18NG-12369-MONOMER.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Synonyms:ack
Ordered Locus Names:Cgl2752, cg3047
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001075531 – 397Acetate kinaseAdd BLAST397

Proteomic databases

PRIDEiP77845.

2D gel databases

World-2DPAGEi0001:P77845.

Expressioni

Inductioni

Activated by RamA and repressed by RamB and GlxR.3 Publications

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg3047.

Structurei

3D structure databases

ProteinModelPortaliP77845.
SMRiP77845.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288398.
KOiK00925.
OMAiWKALTGT.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiView protein in InterPro
IPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiView protein in Pfam
PF00871. Acetate_kinase. 1 hit.
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiView protein in PROSITE
PS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P77845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALALVLNSG SSSIKFQLVN PENSAIDEPY VSGLVEQIGE PNGRIVLKIE
60 70 80 90 100
GEKYTLETPI ADHSEGLNLA FDLMDQHNCG PSQLEITAVG HRVVHGGILF
110 120 130 140 150
SAPELITDEI VEMIRDLIPL APLHNPANVD GIDVARKILP DVPHVAVFDT
160 170 180 190 200
GFFHSLPPAA ALYAINKDVA AEHGIRRYGF HGTSHEFVSK RVVEILEKPT
210 220 230 240 250
EDINTITFHL GNGASMAAVQ GGRAVDTSMG MTPLAGLVMG TRSGDIDPGI
260 270 280 290 300
VFHLSRTAGM SIDEIDNLLN KKSGVKGLSG VNDFRELREM IDNNDQDAWS
310 320 330 340 350
AYNIYIHQLR RYLGSYMVAL GRVDTIVFTA GVGENAQFVR EDALAGLEMY
360 370 380 390
GIEIDPERNA LPNDGPRLIS TDASKVKVFV IPTNEELAIA RYAVKFA
Length:397
Mass (Da):43,092
Last modified:February 1, 1997 - v1
Checksum:i248FF97A3C52B0E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89084 Genomic DNA. Translation: CAA61456.1.
BA000036 Genomic DNA. Translation: BAC00146.1.
BX927156 Genomic DNA. Translation: CAF20774.1.
RefSeqiNP_601947.1. NC_003450.3.
WP_003862874.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAC00146; BAC00146; BAC00146.
CAF20774; CAF20774; cg3047.
GeneIDi1020697.
KEGGicgb:cg3047.
cgl:NCgl2656.
PATRICifig|196627.13.peg.2683.

Similar proteinsi

Entry informationi

Entry nameiACKA_CORGL
AccessioniPrimary (citable) accession number: P77845
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 7, 2017
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. UniProtKB entry view manual
    User manual for the UniProtKB entry view