Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Urease subunit alpha

Gene

ureC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation2 Publications

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi137 – 1371Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi139 – 1391Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi220 – 2201Nickel 1; via carbamate groupUniRule annotation
Metal bindingi220 – 2201Nickel 2; via carbamate groupUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Metal bindingi249 – 2491Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi275 – 2751Nickel 2; via tele nitrogenUniRule annotation
Active sitei323 – 3231Proton donorUniRule annotation
Metal bindingi363 – 3631Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciBSUB:BSU36640-MONOMER.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:BSU36640
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Urease subunit alphaPRO_0000067538Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei220 – 2201N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PaxDbiP77837.

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019806.

Structurei

3D structure databases

ProteinModelPortaliP77837.
SMRiP77837. Positions 3-569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini132 – 569438UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
InParanoidiP77837.
KOiK01428.
OMAiFDSHIHF.
PhylomeDBiP77837.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMSREEYAE LFGPTTGDKI RLGDTDLWIE VEKDFTVYGE EMIFGGGKTI
60 70 80 90 100
RDGMGQNGRI TGKDGALDLV ITNVVLLDYT GIVKADVGVK DGRIVGVGKS
110 120 130 140 150
GNPDIMDGVD PHMVIGAGTE VISGEGKILT AGGVDTHIHF ICPQQMEVAL
160 170 180 190 200
SSGVTTLLGG GTGPATGSKA TTCTSGAWYM ARMLEAAEEF PINVGFLGKG
210 220 230 240 250
NASDKAPLIE QVEAGAIGLK LHEDWGTTPS AIKTCMEVVD EADIQVAIHT
260 270 280 290 300
DTINEAGFLE NTLDAIGDRV IHTYHIEGAG GGHAPDIMKL ASYANILPSS
310 320 330 340 350
TTPTIPYTVN TMDEHLDMMM VCHHLDAKVP EDVAFSHSRI RAATIAAEDI
360 370 380 390 400
LHDIGAISMT SSDSQAMGRV GEVIIRTWQV ADKMKKQRGA LAGENGNDNV
410 420 430 440 450
RAKRYIAKYT INPAITHGLS HEVGSVEKGK LADLVLWDPV FFGVKPELVL
460 470 480 490 500
KGGMIARAQM GDPNASIPTP EPVFMRQMYA SYGKANRSTS ITFMSQASIE
510 520 530 540 550
RGVAESLGLE KRISPVKNIR KLSKLDMKLN SALPKIEIDP KTYQVFADGE
560
ELSCQPVDYV PLGQRYFLF
Length:569
Mass (Da):61,187
Last modified:February 1, 1997 - v1
Checksum:iA2712A6172DA5BE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08559 Genomic DNA. Translation: CAA69859.1.
AL009126 Genomic DNA. Translation: CAB15681.1.
PIRiD69729.
RefSeqiNP_391545.1. NC_000964.3.
WP_003243090.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15681; CAB15681; BSU36640.
GeneIDi936958.
KEGGibsu:BSU36640.
PATRICi18979356. VBIBacSub10457_3840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08559 Genomic DNA. Translation: CAA69859.1.
AL009126 Genomic DNA. Translation: CAB15681.1.
PIRiD69729.
RefSeqiNP_391545.1. NC_000964.3.
WP_003243090.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP77837.
SMRiP77837. Positions 3-569.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019806.

Protein family/group databases

MEROPSiM38.982.

Proteomic databases

PaxDbiP77837.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15681; CAB15681; BSU36640.
GeneIDi936958.
KEGGibsu:BSU36640.
PATRICi18979356. VBIBacSub10457_3840.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
InParanoidiP77837.
KOiK01428.
OMAiFDSHIHF.
PhylomeDBiP77837.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciBSUB:BSU36640-MONOMER.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURE1_BACSU
AccessioniPrimary (citable) accession number: P77837
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.