P77834 (PUNA_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purine nucleoside phosphorylase 1
Short name=PNP 1
EC=2.4.2.1

Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase
Purine nucleoside phosphorylase I
Short name=PNP I
Short name=PU-NPase I

Gene names

Name:	punA
Synonyms:	deoD

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine and inosine. Ref.1
Catalytic activity	Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.
Pathway	Purine metabolism; purine nucleoside salvage.
Sequence similarities	Belongs to the PNP/MTAP phosphorylase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	purine-nucleoside phosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Purine nucleoside phosphorylase 1		PRO_0000184539
Amino acid modifications
	Modified residue	29	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77834 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: EC02BD23DA8A51C5	FASTA	274	29,637
	10 20 30 40 50 60 MNRTAIEQAA QFLKEKFPTS PQIGLILGSG LGVLADEIEQ AIKIPYSDIP NFPVSTVEGH 70 80 90 100 110 120 AGQLVYGQLE GATVVVMQGR FHYYEGYSFD KVTFPVRVMK ALGVEQLIVT NAAGGVNESF 130 140 150 160 170 180 EPGDLMIISD HINNMGGNPL IGPNDSALGV RFPDMSEAYS KRLRQLAKDV ANDIGLRVRE 190 200 210 220 230 240 GVYVANTGPA YETPAEIRMI RVMGGDAVGM STVPEVIVAR HAGMEVLGIS CISNMAAGIL 250 260 270 DQPLTHDEVI ETTEKVKADF LRFVKAIVRN MAKN

References

[1]

"Cloning and expression of purine nucleoside phosphorylase I gene from Bacillus stearothermophilus TH 6-2." Hamamoto T., Okuyama K., Noguchi T., Midorikawa Y. Biosci. Biotechnol. Biochem. 61:272-275(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: TH 6-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87959 Genomic DNA. Translation: BAA13509.1.
PIR	JT0873. JC5466.
3D structure databases
ProteinModelPortal	P77834.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00606.
Family and domain databases
Gene3D	3.40.50.1580. 1 hit.
InterPro	IPR000845. Nucleoside_phosphorylase_d. IPR001369. PNP/MTAP. IPR011270. Pur_Nuc_Pase_Ino/Guo-sp. IPR011268. Purine_phosphorylase. IPR018099. Purine_phosphorylase-2_CS. [Graphical view]
PANTHER	PTHR11904. PTHR11904. 1 hit. PTHR11904:SF9. PTHR11904:SF9. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
PIRSF	PIRSF000477. PurNPase. 1 hit.
TIGRFAMs	TIGR01700. PNPH. 1 hit. TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITE	PS01240. PNP_MTAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUNA_GEOSE

Accession

Primary (citable) accession number: P77834

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program