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P77834 (PUNA_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purine nucleoside phosphorylase 1

Short name=PNP 1
EC=2.4.2.1
Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase
Purine nucleoside phosphorylase I
Short name=PNP I
Short name=PU-NPase I
Gene names
Name:punA
Synonyms:deoD
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine and inosine. Ref.1

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.

Pathway

Purine metabolism; purine nucleoside salvage.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processnucleoside metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionpurine-nucleoside phosphorylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Purine nucleoside phosphorylase 1
PRO_0000184539

Amino acid modifications

Modified residue291Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P77834 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: EC02BD23DA8A51C5

FASTA27429,637
        10         20         30         40         50         60 
MNRTAIEQAA QFLKEKFPTS PQIGLILGSG LGVLADEIEQ AIKIPYSDIP NFPVSTVEGH 

        70         80         90        100        110        120 
AGQLVYGQLE GATVVVMQGR FHYYEGYSFD KVTFPVRVMK ALGVEQLIVT NAAGGVNESF 

       130        140        150        160        170        180 
EPGDLMIISD HINNMGGNPL IGPNDSALGV RFPDMSEAYS KRLRQLAKDV ANDIGLRVRE 

       190        200        210        220        230        240 
GVYVANTGPA YETPAEIRMI RVMGGDAVGM STVPEVIVAR HAGMEVLGIS CISNMAAGIL 

       250        260        270 
DQPLTHDEVI ETTEKVKADF LRFVKAIVRN MAKN 

« Hide

References

[1]"Cloning and expression of purine nucleoside phosphorylase I gene from Bacillus stearothermophilus TH 6-2."
Hamamoto T., Okuyama K., Noguchi T., Midorikawa Y.
Biosci. Biotechnol. Biochem. 61:272-275(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: TH 6-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87959 Genomic DNA. Translation: BAA13509.1.
PIRJT0873. JC5466.

3D structure databases

ProteinModelPortalP77834.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00606.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
InterProIPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR011268. Purine_phosphorylase.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PTHR11904:SF9. PTHR11904:SF9. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
PIRSFPIRSF000477. PurNPase. 1 hit.
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITEPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUNA_GEOSE
AccessionPrimary (citable) accession number: P77834
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways