Reviewed, UniProtKB/Swiss-Prot P77816 (SDIS_NOCSI)

Names and origin

Protein names

Recommended name:
    Steroid Delta-isomerase
    EC=5.3.3.1
Alternative name(s):
    Delta(5)-3-ketosteroid isomerase

Gene names

Name:

ksdI

Organism

Nocardioides simplex (Arthrobacter simplex)

Taxonomic identifier

2045 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Pimelobacter

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Subunit structure	Homodimer By similarity.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 123	123	Steroid Delta-isomerase		PRO_0000097645
Sites
	Active site	12	1	Proton donor By similarity
	Active site	36	1	Proton acceptor By similarity
	Binding site	96	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77816-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: F08AF72E2F3858B6	FASTA	123	12,677
	10 20 30 40 50 60 MSAEVKAAVA RYLDAVAGGS PAAIAALYAP DATLEDPVGA DLVRGRAAIE EFYGALAGAK 70 80 90 100 110 120 VSTELLAVRA VAGHAAFSFR VTTDAGDQQY VVEPIDVMTF DADGQITSMR AFWAPGDMVV TPA

References

[1]

"Molecular cloning, expression in Streptomyces lividans, and analysis of a gene cluster from Arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein." Molnar I., Choi K.P., Yamashita M., Murooka Y. Mol. Microbiol. 15:895-905(1995) [PubMed: 7596291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6946 / DSM 20130 / IFO 12069 / JCM 1363 / NCIB 8929.

[2]

Dziadek J., Yamashita M., Murooka Y. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6946 / DSM 20130 / IFO 12069 / JCM 1363 / NCIB 8929.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D37969 Genomic DNA. Translation: BAA07187.1. Z93338 Genomic DNA. Translation: CAB07540.1.
PIR	S61890.
3D structure databases
HSSP	HSSP built from PDB template 1OPY based on UniProtKB P07445.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.3.3.1. 256078.
Family and domain databases
InterPro	IPR002075. NTF2. [Graphical view]
Pfam	PF02136. NTF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SDIS_NOCSI

Accession

Primary (citable) accession number: P77816
Secondary accession number(s): O08058

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 5, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)