P77816 (SDIS_NOCSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Steroid Delta-isomerase
EC=5.3.3.1

Alternative name(s):
Delta(5)-3-ketosteroid isomerase

Gene names

Name:

ksdI

Organism

Nocardioides simplex (Arthrobacter simplex)

Taxonomic identifier

2045 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Pimelobacter

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Subunit structure	Homodimer By similarity.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 123	123	Steroid Delta-isomerase		PRO_0000097645
Sites
	Active site	12	1	Proton donor By similarity
	Active site	36	1	Proton acceptor By similarity
	Binding site	96	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77816 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: F08AF72E2F3858B6	FASTA	123	12,677
	10 20 30 40 50 60 MSAEVKAAVA RYLDAVAGGS PAAIAALYAP DATLEDPVGA DLVRGRAAIE EFYGALAGAK 70 80 90 100 110 120 VSTELLAVRA VAGHAAFSFR VTTDAGDQQY VVEPIDVMTF DADGQITSMR AFWAPGDMVV TPA

References

[1]	"Molecular cloning, expression in Streptomyces lividans, and analysis of a gene cluster from Arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein." Molnar I., Choi K.P., Yamashita M., Murooka Y. Mol. Microbiol. 15:895-905(1995) [PubMed: 7596291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6946 / DSM 20130 / JCM 1363 / NBRC 12069 / NCIB 8929 / NCTC 4215 / NRRL B-14051.
[2]	Dziadek J., Yamashita M., Murooka Y. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6946 / DSM 20130 / JCM 1363 / NBRC 12069 / NCIB 8929 / NCTC 4215 / NRRL B-14051.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D37969 Genomic DNA. Translation: BAA07187.1. Z93338 Genomic DNA. Translation: CAB07540.1.
PIR	S61890.
3D structure databases
ProteinModelPortal	P77816.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002075. NTF2. [Graphical view]
Pfam	PF02136. NTF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SDIS_NOCSI

Accession

Primary (citable) accession number: P77816
Secondary accession number(s): O08058

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	September 21, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program