P77810 (LON_AZOBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:

lon

Organism

Azospirillum brasilense

Taxonomic identifier

192 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity. Involved in iron uptake.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm.
Induction	By heat shock. Ref.1
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 810	810	Lon protease		PRO_0000076115
Regions
	Domain	17 – 206	190	Lon
	Nucleotide binding	361 – 368	8	ATP By similarity
Sites
	Active site	685	1	By similarity
	Active site	728	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P77810 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 2833882C69886C4B	FASTA	810	90,143
	10 20 30 40 50 60 MKEAQSMFEI PRGALYPVPP LRDIVVFPHM IVPLFVGREK SVRALEDVMK DDKQILLVTQ 70 80 90 100 110 120 KNAAQDDPTP ADIYSVGTVG TVLQLLKLPD GTVKVLVEGG QRASITKFAE NEDFFQAHAD 130 140 150 160 170 180 LVEEKVGESQ ELEALGRAVV SQFEQYIKLN KKIPPEVLVS INQIEEPGKL ADTVASHLAL 190 200 210 220 230 240 KIPEKQQLLE CATVSERLER VYAFMEGEIG VLQVEKRIRN RVKRQMEKTQ REYYLNEQLK 250 260 270 280 290 300 AIQKELGETE DGRDESAELE EKINKTRFSK EARDKALAEL KKLRSMSPMS AEATVVRNYL 310 320 330 340 350 360 DWMLSIPWKK RTKVKKDLKL AQKILDADHY GLEKVKERIL EYLRVQNRMN KVKGPIQSLV 370 380 390 400 410 420 GPPGVGKTSL GKSIAKSTGR NFVRMSLGGV RDEAEVRGHR RTYIGSMPGK VIQGMKKAKS 430 440 450 460 470 480 SNPLFLLDEI DKLGADWRGD PSSALLEVLD PEQNGTFNDH YLEVDYDLSD VMFVCTANTM 490 500 510 520 530 540 RMPQPLLDRM EIIRVAGYTE DEKVEISKRH LIEKQVEANG LKKGEFAISD DALRDLIRYY 550 560 570 580 590 600 TREAGVRSLE REIANLCRKA VKEILMKGSA GAKVSVTRRN LDKYAGVRRF HFGEAELEDL 610 620 630 640 650 660 VGVTTGLAWT EVGGELLSIE AVSLPGKGRV TTTGKLGDVM KESVQAAESY VKSRATAFGI 670 680 690 700 710 720 KPTLFEKRDI HVHVPEGATP KDGPSAGVAM ITSIVSVLTG IAVRKDVAMT GEITLRGRVL 730 740 750 760 770 780 PIGGLKEKLL AALRGGLKHV LIPKDNEKDL AEIPDNVKRG LEIIPVSTVD DVLKHALVRE 790 800 810 VEPIEWKEPE AVEPAVAKPQ TDGGGEVLRH

References

[1]

"Cloning, nucleotide sequencing, and expression of the Azospirillum brasilense lon gene: involvement in iron uptake." Mori E., Fulchieri M., Indorato C., Fani R., Bazzicalupo M. J. Bacteriol. 178:3440-3446(1996) [PubMed: 8655539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: SpF94.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35611 Genomic DNA. Translation: AAB16819.1.
PIR	JC6045.
3D structure databases
ProteinModelPortal	P77810.
SMR	P77810. Positions 17-120, 496-592, 601-780.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_AZOBR

Accession

Primary (citable) accession number: P77810

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program