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Protein

Methionine aminotransferase

Gene

ybdL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Shows aminotransferase activity with methionine and histidine as substrates, and to a lesser extent also with phenylalanine.1 Publication

Catalytic activityi

L-methionine + a 2-oxo acid = 2-oxo-4-methylthiobutanoate + an L-amino acid.1 Publication

Cofactori

GO - Molecular functioni

  • methionine-oxo-acid transaminase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:G6329-MONOMER.
ECOL316407:JW0593-MONOMER.
MetaCyc:G6329-MONOMER.
BRENDAi2.6.1.88. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminotransferase (EC:2.6.1.88)
Alternative name(s):
Methionine-oxo-acid transaminase
Gene namesi
Name:ybdL
Ordered Locus Names:b0600, JW0593
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13531. ybdL.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Methionine aminotransferasePRO_0000123925Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361N6-(pyridoxal phosphate)lysine

Proteomic databases

PaxDbiP77806.
PRIDEiP77806.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
adkP694412EBI-543661,EBI-543592
cyoBP0ABI83EBI-543661,EBI-2932021
fixXP686463EBI-543661,EBI-1113234

Protein-protein interaction databases

BioGridi4260981. 13 interactions.
DIPiDIP-11350N.
IntActiP77806. 14 interactions.
MINTiMINT-1306557.
STRINGi511145.b0600.

Structurei

Secondary structure

1
386
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi19 – 2911Combined sources
Helixi46 – 5712Combined sources
Helixi70 – 8415Combined sources
Turni90 – 934Combined sources
Beta strandi94 – 996Combined sources
Helixi100 – 11112Combined sources
Beta strandi117 – 1237Combined sources
Helixi128 – 1347Combined sources
Beta strandi138 – 1436Combined sources
Turni146 – 1483Combined sources
Helixi153 – 1597Combined sources
Beta strandi164 – 1729Combined sources
Turni174 – 1763Combined sources
Helixi182 – 19211Combined sources
Beta strandi198 – 2025Combined sources
Turni204 – 2074Combined sources
Helixi218 – 2203Combined sources
Helixi222 – 2254Combined sources
Beta strandi228 – 2336Combined sources
Helixi234 – 2374Combined sources
Helixi241 – 2433Combined sources
Beta strandi246 – 2494Combined sources
Helixi252 – 26514Combined sources
Helixi271 – 28313Combined sources
Helixi286 – 2894Combined sources
Helixi291 – 30515Combined sources
Beta strandi308 – 3103Combined sources
Beta strandi318 – 3258Combined sources
Turni327 – 3293Combined sources
Helixi334 – 34411Combined sources
Helixi352 – 3554Combined sources
Beta strandi364 – 3685Combined sources
Helixi373 – 38311Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U08X-ray2.35A/B1-386[»]
ProteinModelPortaliP77806.
SMRiP77806. Positions 5-386.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77806.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHM. Bacteria.
COG0436. LUCA.
HOGENOMiHOG000223045.
InParanoidiP77806.
KOiK14287.
OMAiSQGANQY.
PhylomeDBiP77806.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

P77806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNNPLIPQS KLPQLGTTIF TQMSALAQQH QAINLSQGFP DFDGPRYLQE
60 70 80 90 100
RLAHHVAQGA NQYAPMTGVQ ALREAIAQKT ERLYGYQPDA DSDITVTAGA
110 120 130 140 150
TEALYAAITA LVRNGDEVIC FDPSYDSYAP AIALSGGIVK RMALQPPHFR
160 170 180 190 200
VDWQEFAALL SERTRLVILN TPHNPSATVW QQADFAALWQ AIAGHEIFVI
210 220 230 240 250
SDEVYEHINF SQQGHASVLA HPQLRERAVA VSSFGKTYHM TGWKVGYCVA
260 270 280 290 300
PAPISAEIRK VHQYLTFSVN TPAQLALADM LRAEPEHYLA LPDFYRQKRD
310 320 330 340 350
ILVNALNESR LEILPCEGTY FLLVDYSAVS TLDDVEFCQW LTQEHGVAAI
360 370 380
PLSVFCADPF PHKLIRLCFA KKESTLLAAA ERLRQL
Length:386
Mass (Da):42,963
Last modified:February 1, 1997 - v1
Checksum:iA95A782EA910B13F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40801.1.
U00096 Genomic DNA. Translation: AAC73701.1.
AP009048 Genomic DNA. Translation: BAA35230.1.
PIRiF64793.
RefSeqiNP_415133.1. NC_000913.3.
WP_000183907.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73701; AAC73701; b0600.
BAA35230; BAA35230; BAA35230.
GeneIDi945211.
KEGGiecj:JW0593.
eco:b0600.
PATRICi32116378. VBIEscCol129921_0629.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40801.1.
U00096 Genomic DNA. Translation: AAC73701.1.
AP009048 Genomic DNA. Translation: BAA35230.1.
PIRiF64793.
RefSeqiNP_415133.1. NC_000913.3.
WP_000183907.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U08X-ray2.35A/B1-386[»]
ProteinModelPortaliP77806.
SMRiP77806. Positions 5-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260981. 13 interactions.
DIPiDIP-11350N.
IntActiP77806. 14 interactions.
MINTiMINT-1306557.
STRINGi511145.b0600.

Proteomic databases

PaxDbiP77806.
PRIDEiP77806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73701; AAC73701; b0600.
BAA35230; BAA35230; BAA35230.
GeneIDi945211.
KEGGiecj:JW0593.
eco:b0600.
PATRICi32116378. VBIEscCol129921_0629.

Organism-specific databases

EchoBASEiEB3302.
EcoGeneiEG13531. ybdL.

Phylogenomic databases

eggNOGiENOG4105CHM. Bacteria.
COG0436. LUCA.
HOGENOMiHOG000223045.
InParanoidiP77806.
KOiK14287.
OMAiSQGANQY.
PhylomeDBiP77806.

Enzyme and pathway databases

BioCyciEcoCyc:G6329-MONOMER.
ECOL316407:JW0593-MONOMER.
MetaCyc:G6329-MONOMER.
BRENDAi2.6.1.88. 2026.

Miscellaneous databases

EvolutionaryTraceiP77806.
PROiP77806.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYBDL_ECOLI
AccessioniPrimary (citable) accession number: P77806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.