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Protein

Probable dimethyl sulfoxide reductase chain YnfF

Gene

ynfF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi63Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi67Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi99Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi195MolybdenumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:G6846-MONOMER
MetaCyc:G6846-MONOMER

Protein family/group databases

TCDBi5.A.3.3.1 the prokaryotic molybdopterin-containing oxidoreductase (pmo) family

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dimethyl sulfoxide reductase chain YnfF (EC:1.8.99.-)
Short name:
DMSO reductase
Gene namesi
Name:ynfF
Ordered Locus Names:b1588, JW5260
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13844 ynfF

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 45Tat-type signalPROSITE-ProRule annotationAdd BLAST45
ChainiPRO_000001914846 – 807Probable dimethyl sulfoxide reductase chain YnfFAdd BLAST762

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP77783
PRIDEiP77783

Interactioni

Subunit structurei

The complex consists of three subunits: YnfF, the reductase; YnfG, an electron transfer protein, and YnfH, a membrane anchor protein.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
dmsDP698533EBI-6406285,EBI-4406374

Protein-protein interaction databases

BioGridi4259130, 9 interactors
DIPiDIP-12766N
IntActiP77783, 2 interactors
STRINGi316385.ECDH10B_1721

Structurei

3D structure databases

ProteinModelPortaliP77783
SMRiP77783
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 1134Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C3J Bacteria
COG1104 LUCA
HOGENOMiHOG000284390
InParanoidiP77783
KOiK07310
OMAiHTQGHTV
PhylomeDBiP77783

Family and domain databases

InterProiView protein in InterPro
IPR011888 Anaer_DMSO_reductase
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR006655 Mopterin_OxRdtase_prok_CS
IPR027467 MopterinOxRdtase_cofactor_BS
IPR006311 TAT_signal
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR02166 dmsA_ynfE, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS00932 MOLYBDOPTERIN_PROK_3, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIHTTEALM KAEISRRSLM KTSALGSLAL ASSAFTLPFS QMVRAAEAPV
60 70 80 90 100
EEKAVWSSCT VNCGSRCLLR LHVKDDTVYW VESDTTGDDV YGNHQVRACL
110 120 130 140 150
RGRSIRRRMN HPDRLKYPMK RVGKRGEGKF ERISWDEALD TISDNLRRIL
160 170 180 190 200
KDYGNEAVHV LYGTGVDGGN ITNSNVPYRL MNSCGGFLSR YGSYSTAQIS
210 220 230 240 250
AAMSYMFGAN DGNSPDDIAN TKLVVMFGNN PAETRMSGGG VTYYVEQARE
260 270 280 290 300
RSNARMIVID PRYNDTAAGR EDEWLPIRPG TDGALACAIA WVLITENMVD
310 320 330 340 350
QPFLDKYCVG YDEKTLPANA PRNAHYKAYI LGEGPDGIAK TPEWAAKITS
360 370 380 390 400
IPAEKIIQLA REIGSAKPAY ICQGWGPQRH SNGEQTSRAI AMLSVLTGNV
410 420 430 440 450
GINGGNSGVR EGSWDLGVEW FPMLENPVKT QISVFTWTDA IDHGTEMTAT
460 470 480 490 500
RDGVRGKEKL DVPIKFLWCY ASNTLINQHG DINHTHEVLQ DDSKCEMIVG
510 520 530 540 550
IDHFMTASAK YCDILLPDLM PTEQEDLISH ESAGNMGYVI LAQPATSAKF
560 570 580 590 600
ERKPIYWMLS EVAKRLGPDV YQTFTEGRSQ HEWIKYLHAK TKERNPEMPD
610 620 630 640 650
YEEMKTTGIF KKKCPEEHYV AFRAFREDPQ ANPLKTPSGK IEIYSERLAK
660 670 680 690 700
IADTWELKKD EIIHPLPAYT PGFDGWDDPL RKTYPLQLTG FHYKARTHSS
710 720 730 740 750
YGNIDVLQQA CPQEVWINPI DAQARGIRHG DTVRVFNNNG EMLIAAKVTP
760 770 780 790 800
RILPGVTAIG QGAWLKADMF GDRVDHGGSI NILTSHRPSP LAKGNPSHSN

LVQIEKV
Length:807
Mass (Da):89,987
Last modified:July 22, 2008 - v4
Checksum:i8120594A8C5816C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74660.4
AP009048 Genomic DNA Translation: BAA15312.2
PIRiF64914
RefSeqiNP_416105.4, NC_000913.3
WP_001340362.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74660; AAC74660; b1588
BAA15312; BAA15312; BAA15312
GeneIDi945268
KEGGiecj:JW5260
eco:b1588
PATRICifig|511145.12.peg.1659

Similar proteinsi

Entry informationi

Entry nameiYNFF_ECOLI
AccessioniPrimary (citable) accession number: P77783
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 22, 2008
Last modified: March 28, 2018
This is version 157 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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