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Protein

Probable dimethyl sulfoxide reductase chain YnfF

Gene

ynfF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi63 – 631Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi67 – 671Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi99 – 991Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi195 – 1951MolybdenumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:G6846-MONOMER.
ECOL316407:JW5260-MONOMER.
MetaCyc:G6846-MONOMER.

Protein family/group databases

TCDBi5.A.3.3.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dimethyl sulfoxide reductase chain YnfF (EC:1.8.99.-)
Short name:
DMSO reductase
Gene namesi
Name:ynfF
Ordered Locus Names:b1588, JW5260
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13844. ynfF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4545Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini46 – 807762Probable dimethyl sulfoxide reductase chain YnfFPRO_0000019148Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP77783.
PRIDEiP77783.

Interactioni

Subunit structurei

The complex consists of three subunits: YnfF, the reductase; YnfG, an electron transfer protein, and YnfH, a membrane anchor protein.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
dmsDP698532EBI-6406285,EBI-4406374

Protein-protein interaction databases

BioGridi4259130. 5 interactions.
DIPiDIP-12766N.
IntActiP77783. 1 interaction.
STRINGi511145.b1588.

Structurei

3D structure databases

ProteinModelPortaliP77783.
SMRiP77783. Positions 52-806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 113624Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C3J. Bacteria.
COG1104. LUCA.
HOGENOMiHOG000284390.
InParanoidiP77783.
KOiK07310.
OMAiINHTNEV.
PhylomeDBiP77783.

Family and domain databases

InterProiIPR011888. Anaer_DMSO_reductase.
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR02166. dmsA_ynfE. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIHTTEALM KAEISRRSLM KTSALGSLAL ASSAFTLPFS QMVRAAEAPV
60 70 80 90 100
EEKAVWSSCT VNCGSRCLLR LHVKDDTVYW VESDTTGDDV YGNHQVRACL
110 120 130 140 150
RGRSIRRRMN HPDRLKYPMK RVGKRGEGKF ERISWDEALD TISDNLRRIL
160 170 180 190 200
KDYGNEAVHV LYGTGVDGGN ITNSNVPYRL MNSCGGFLSR YGSYSTAQIS
210 220 230 240 250
AAMSYMFGAN DGNSPDDIAN TKLVVMFGNN PAETRMSGGG VTYYVEQARE
260 270 280 290 300
RSNARMIVID PRYNDTAAGR EDEWLPIRPG TDGALACAIA WVLITENMVD
310 320 330 340 350
QPFLDKYCVG YDEKTLPANA PRNAHYKAYI LGEGPDGIAK TPEWAAKITS
360 370 380 390 400
IPAEKIIQLA REIGSAKPAY ICQGWGPQRH SNGEQTSRAI AMLSVLTGNV
410 420 430 440 450
GINGGNSGVR EGSWDLGVEW FPMLENPVKT QISVFTWTDA IDHGTEMTAT
460 470 480 490 500
RDGVRGKEKL DVPIKFLWCY ASNTLINQHG DINHTHEVLQ DDSKCEMIVG
510 520 530 540 550
IDHFMTASAK YCDILLPDLM PTEQEDLISH ESAGNMGYVI LAQPATSAKF
560 570 580 590 600
ERKPIYWMLS EVAKRLGPDV YQTFTEGRSQ HEWIKYLHAK TKERNPEMPD
610 620 630 640 650
YEEMKTTGIF KKKCPEEHYV AFRAFREDPQ ANPLKTPSGK IEIYSERLAK
660 670 680 690 700
IADTWELKKD EIIHPLPAYT PGFDGWDDPL RKTYPLQLTG FHYKARTHSS
710 720 730 740 750
YGNIDVLQQA CPQEVWINPI DAQARGIRHG DTVRVFNNNG EMLIAAKVTP
760 770 780 790 800
RILPGVTAIG QGAWLKADMF GDRVDHGGSI NILTSHRPSP LAKGNPSHSN

LVQIEKV
Length:807
Mass (Da):89,987
Last modified:July 22, 2008 - v4
Checksum:i8120594A8C5816C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74660.4.
AP009048 Genomic DNA. Translation: BAA15312.2.
PIRiF64914.
RefSeqiNP_416105.4. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74660; AAC74660; b1588.
BAA15312; BAA15312; BAA15312.
GeneIDi945268.
KEGGiecj:JW5260.
eco:b1588.
PATRICi32118478. VBIEscCol129921_1659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74660.4.
AP009048 Genomic DNA. Translation: BAA15312.2.
PIRiF64914.
RefSeqiNP_416105.4. NC_000913.3.

3D structure databases

ProteinModelPortaliP77783.
SMRiP77783. Positions 52-806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259130. 5 interactions.
DIPiDIP-12766N.
IntActiP77783. 1 interaction.
STRINGi511145.b1588.

Protein family/group databases

TCDBi5.A.3.3.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Proteomic databases

PaxDbiP77783.
PRIDEiP77783.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74660; AAC74660; b1588.
BAA15312; BAA15312; BAA15312.
GeneIDi945268.
KEGGiecj:JW5260.
eco:b1588.
PATRICi32118478. VBIEscCol129921_1659.

Organism-specific databases

EchoBASEiEB3605.
EcoGeneiEG13844. ynfF.

Phylogenomic databases

eggNOGiENOG4105C3J. Bacteria.
COG1104. LUCA.
HOGENOMiHOG000284390.
InParanoidiP77783.
KOiK07310.
OMAiINHTNEV.
PhylomeDBiP77783.

Enzyme and pathway databases

BioCyciEcoCyc:G6846-MONOMER.
ECOL316407:JW5260-MONOMER.
MetaCyc:G6846-MONOMER.

Miscellaneous databases

PROiP77783.

Family and domain databases

InterProiIPR011888. Anaer_DMSO_reductase.
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR02166. dmsA_ynfE. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYNFF_ECOLI
AccessioniPrimary (citable) accession number: P77783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 22, 2008
Last modified: September 7, 2016
This is version 146 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.