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Protein

Outer membrane protein assembly factor BamB

Gene

bamB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins. Efficient substrate folding and insertion into the outer membrane requires all 5 subunits (PubMed:20378773, PubMed:21823654, PubMed:27686148). A lateral gate may open between the first and last strands of the BamA beta-barrel that allows substrate to insert into the outer membrane; comparison of the structures of complete and nearly complete Bam complexes show there is considerable movement of all 5 proteins (PubMed:27686148, PubMed:26900875, PubMed:26901871).7 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • oxidoreductase activity Source: GO_Central

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
  • protein insertion into membrane Source: EcoCyc

Enzyme and pathway databases

BioCyciEcoCyc:G7320-MONOMER.

Protein family/group databases

TCDBi1.B.33.1.3. the outer membrane protein insertion porin (bam complex) (ompip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein assembly factor BamBUniRule annotation
Gene namesi
Name:bamBUniRule annotation
Synonyms:yfgL
Ordered Locus Names:b2512, JW2496
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14208. bamB.

Subcellular locationi

GO - Cellular componenti

  • Bam protein complex Source: EcoCyc

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19UniRule annotationAdd BLAST19
ChainiPRO_000004221120 – 392Outer membrane protein assembly factor BamBAdd BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi20N-palmitoyl cysteine1 Publication1
Lipidationi20S-diacylglycerol cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP77774.
PRIDEiP77774.

Interactioni

Subunit structurei

Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE. Monomer. Interacts directly with BamA. The Bam complex has the shape of a hat, with the BamA beta-barrel crown in the outer membrane and the periplasmic brim formed by the BamA POTRA domains and the 4 lipoproteins (PubMed:27686148, PubMed:26900875, PubMed:26901871).9 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260587. 290 interactors.
DIPiDIP-12042N.
IntActiP77774. 8 interactors.
STRINGi316385.ECDH10B_2678.

Structurei

Secondary structure

1392
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 26Combined sources5
Beta strandi45 – 50Combined sources6
Turni53 – 58Combined sources6
Beta strandi66 – 68Combined sources3
Beta strandi71 – 75Combined sources5
Beta strandi79 – 85Combined sources7
Turni86 – 88Combined sources3
Beta strandi91 – 96Combined sources6
Beta strandi99 – 105Combined sources7
Beta strandi111 – 118Combined sources8
Beta strandi121 – 126Combined sources6
Beta strandi129 – 135Combined sources7
Turni136 – 138Combined sources3
Beta strandi141 – 146Combined sources6
Beta strandi156 – 158Combined sources3
Beta strandi161 – 165Combined sources5
Beta strandi169 – 175Combined sources7
Turni176 – 178Combined sources3
Beta strandi181 – 186Combined sources6
Beta strandi191 – 195Combined sources5
Beta strandi201 – 203Combined sources3
Beta strandi206 – 209Combined sources4
Turni212 – 214Combined sources3
Beta strandi215 – 220Combined sources6
Turni221 – 223Combined sources3
Beta strandi226 – 231Combined sources6
Beta strandi252 – 254Combined sources3
Beta strandi257 – 261Combined sources5
Beta strandi267 – 271Combined sources5
Turni272 – 274Combined sources3
Beta strandi277 – 281Combined sources5
Beta strandi286 – 292Combined sources7
Beta strandi295 – 300Combined sources6
Beta strandi305 – 309Combined sources5
Turni310 – 312Combined sources3
Beta strandi315 – 319Combined sources5
Turni321 – 324Combined sources4
Beta strandi331 – 333Combined sources3
Beta strandi336 – 340Combined sources5
Beta strandi344 – 350Combined sources7
Turni351 – 353Combined sources3
Beta strandi356 – 361Combined sources6
Beta strandi372 – 374Combined sources3
Beta strandi377 – 381Combined sources5
Beta strandi383 – 385Combined sources3
Beta strandi387 – 391Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YH3X-ray2.60A22-392[»]
2YMSX-ray2.10A62-191[»]
B113-186[»]
C248-322[»]
D247-320[»]
3P1LX-ray2.60A21-392[»]
3PRWX-ray1.80A21-392[»]
3Q7MX-ray1.65A21-392[»]
3Q7NX-ray1.77A21-392[»]
3Q7OX-ray2.09A21-392[»]
4PK1X-ray3.10A21-392[»]
4XGAX-ray2.15A20-392[»]
5AYWX-ray3.56B20-392[»]
5D0OX-ray2.90B1-392[»]
5LJOelectron microscopy4.90B22-392[»]
ProteinModelPortaliP77774.
SMRiP77774.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77774.

Family & Domainsi

Sequence similaritiesi

Belongs to the BamB family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CNE. Bacteria.
COG1520. LUCA.
HOGENOMiHOG000260994.
InParanoidiP77774.
KOiK17713.
PhylomeDBiP77774.

Family and domain databases

HAMAPiMF_00923. OM_assembly_BamB. 1 hit.
InterProiView protein in InterPro
IPR017687. BamB.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR011047. Quinoprotein_ADH-like_supfam.
PfamiView protein in Pfam
PF13360. PQQ_2. 1 hit.
SMARTiView protein in SMART
SM00564. PQQ. 7 hits.
SUPFAMiSSF50998. SSF50998. 1 hit.
TIGRFAMsiTIGR03300. assembly_YfgL. 1 hit.
PROSITEiView protein in PROSITE
PS51257. PROKAR_LIPOPROTEIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLRKLLLPG LLSVTLLSGC SLFNSEEDVV KMSPLPTVEN QFTPTTAWST
60 70 80 90 100
SVGSGIGNFY SNLHPALADN VVYAADRAGL VKALNADDGK EIWSVSLAEK
110 120 130 140 150
DGWFSKEPAL LSGGVTVSGG HVYIGSEKAQ VYALNTSDGT VAWQTKVAGE
160 170 180 190 200
ALSRPVVSDG LVLIHTSNGQ LQALNEADGA VKWTVNLDMP SLSLRGESAP
210 220 230 240 250
TTAFGAAVVG GDNGRVSAVL MEQGQMIWQQ RISQATGSTE IDRLSDVDTT
260 270 280 290 300
PVVVNGVVFA LAYNGNLTAL DLRSGQIMWK RELGSVNDFI VDGNRIYLVD
310 320 330 340 350
QNDRVMALTI DGGVTLWTQS DLLHRLLTSP VLYNGNLVVG DSEGYLHWIN
360 370 380 390
VEDGRFVAQQ KVDSSGFQTE PVAADGKLLI QAKDGTVYSI TR
Length:392
Mass (Da):41,887
Last modified:February 1, 1997 - v1
Checksum:iBCFAFA061A486B31
GO

Mass spectrometryi

Molecular mass is 40643 Da from positions 22 - 392. Determined by ESI. With 3 palmitic acid (C16) acyl chains.1 Publication
Molecular mass is 40663 Da from positions 22 - 392. Determined by ESI. With 2 palmitic (C16) and 1 stearic (C18) acid acyl chains.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75565.1.
AP009048 Genomic DNA. Translation: BAA16398.1.
PIRiG65027.
RefSeqiNP_417007.1. NC_000913.3.
WP_001177052.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75565; AAC75565; b2512.
BAA16398; BAA16398; BAA16398.
GeneIDi946982.
KEGGiecj:JW2496.
eco:b2512.
PATRICifig|1411691.4.peg.4224.

Similar proteinsi

Entry informationi

Entry nameiBAMB_ECOLI
AccessioniPrimary (citable) accession number: P77774
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: September 27, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families