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Protein

Outer membrane protein assembly factor BamB

Gene

bamB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.UniRule annotation4 Publications

GO - Molecular functioni

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
  • protein insertion into membrane Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7320-MONOMER.
ECOL316407:JW2496-MONOMER.

Protein family/group databases

TCDBi1.B.33.1.3. the outer membrane protein insertion porin (bam complex) (ompip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein assembly factor BamBUniRule annotation
Gene namesi
Name:bamBUniRule annotation
Synonyms:yfgL
Ordered Locus Names:b2512, JW2496
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14208. bamB.

Subcellular locationi

  • Cell outer membrane UniRule annotation1 Publication; Lipid-anchor UniRule annotation1 Publication

GO - Cellular componenti

  • Bam protein complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19UniRule annotationAdd BLAST19
ChainiPRO_000004221120 – 392Outer membrane protein assembly factor BamBAdd BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi20N-palmitoyl cysteineCurated1
Lipidationi20S-diacylglycerol cysteineCurated1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP77774.
PaxDbiP77774.
PRIDEiP77774.

Interactioni

Subunit structurei

Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE. Monomer. Interacts directly with BamA.UniRule annotation6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-907297,EBI-907297
bamAP0A94016EBI-907297,EBI-907371
surAP0ABZ62EBI-907297,EBI-558651

Protein-protein interaction databases

BioGridi4260587. 208 interactors.
DIPiDIP-12042N.
IntActiP77774. 8 interactors.
STRINGi511145.b2512.

Structurei

Secondary structure

1392
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 26Combined sources5
Beta strandi45 – 50Combined sources6
Turni53 – 58Combined sources6
Beta strandi66 – 68Combined sources3
Beta strandi71 – 75Combined sources5
Beta strandi79 – 85Combined sources7
Turni86 – 88Combined sources3
Beta strandi91 – 96Combined sources6
Beta strandi99 – 105Combined sources7
Beta strandi111 – 118Combined sources8
Beta strandi121 – 126Combined sources6
Beta strandi129 – 135Combined sources7
Turni136 – 138Combined sources3
Beta strandi141 – 146Combined sources6
Beta strandi156 – 158Combined sources3
Beta strandi161 – 165Combined sources5
Beta strandi169 – 175Combined sources7
Turni176 – 178Combined sources3
Beta strandi181 – 186Combined sources6
Beta strandi191 – 195Combined sources5
Beta strandi201 – 203Combined sources3
Beta strandi206 – 209Combined sources4
Turni212 – 214Combined sources3
Beta strandi215 – 220Combined sources6
Turni221 – 223Combined sources3
Beta strandi226 – 231Combined sources6
Beta strandi252 – 254Combined sources3
Beta strandi257 – 261Combined sources5
Beta strandi267 – 271Combined sources5
Turni272 – 274Combined sources3
Beta strandi277 – 281Combined sources5
Beta strandi286 – 292Combined sources7
Beta strandi295 – 300Combined sources6
Beta strandi305 – 309Combined sources5
Turni310 – 312Combined sources3
Beta strandi315 – 319Combined sources5
Turni321 – 324Combined sources4
Beta strandi331 – 333Combined sources3
Beta strandi336 – 340Combined sources5
Beta strandi344 – 350Combined sources7
Turni351 – 353Combined sources3
Beta strandi356 – 361Combined sources6
Beta strandi372 – 374Combined sources3
Beta strandi377 – 381Combined sources5
Beta strandi383 – 385Combined sources3
Beta strandi387 – 391Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YH3X-ray2.60A22-392[»]
2YMSX-ray2.10A62-191[»]
B113-186[»]
C248-322[»]
D247-320[»]
3P1LX-ray2.60A21-392[»]
3PRWX-ray1.80A21-392[»]
3Q7MX-ray1.65A21-392[»]
3Q7NX-ray1.77A21-392[»]
3Q7OX-ray2.09A21-392[»]
4PK1X-ray3.10A21-392[»]
4XGAX-ray2.15A20-392[»]
5AYWX-ray3.56B20-392[»]
5D0OX-ray2.90B1-392[»]
5LJOelectron microscopy4.90B22-392[»]
ProteinModelPortaliP77774.
SMRiP77774.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77774.

Family & Domainsi

Sequence similaritiesi

Belongs to the BamB family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CNE. Bacteria.
COG1520. LUCA.
HOGENOMiHOG000260994.
InParanoidiP77774.
KOiK17713.
OMAiFTPTKVW.
PhylomeDBiP77774.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
HAMAPiMF_00923. OM_assembly_BamB. 1 hit.
InterProiIPR017687. BamB.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
[Graphical view]
PfamiPF13360. PQQ_2. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 7 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
TIGRFAMsiTIGR03300. assembly_YfgL. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLRKLLLPG LLSVTLLSGC SLFNSEEDVV KMSPLPTVEN QFTPTTAWST
60 70 80 90 100
SVGSGIGNFY SNLHPALADN VVYAADRAGL VKALNADDGK EIWSVSLAEK
110 120 130 140 150
DGWFSKEPAL LSGGVTVSGG HVYIGSEKAQ VYALNTSDGT VAWQTKVAGE
160 170 180 190 200
ALSRPVVSDG LVLIHTSNGQ LQALNEADGA VKWTVNLDMP SLSLRGESAP
210 220 230 240 250
TTAFGAAVVG GDNGRVSAVL MEQGQMIWQQ RISQATGSTE IDRLSDVDTT
260 270 280 290 300
PVVVNGVVFA LAYNGNLTAL DLRSGQIMWK RELGSVNDFI VDGNRIYLVD
310 320 330 340 350
QNDRVMALTI DGGVTLWTQS DLLHRLLTSP VLYNGNLVVG DSEGYLHWIN
360 370 380 390
VEDGRFVAQQ KVDSSGFQTE PVAADGKLLI QAKDGTVYSI TR
Length:392
Mass (Da):41,887
Last modified:February 1, 1997 - v1
Checksum:iBCFAFA061A486B31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75565.1.
AP009048 Genomic DNA. Translation: BAA16398.1.
PIRiG65027.
RefSeqiNP_417007.1. NC_000913.3.
WP_001177052.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75565; AAC75565; b2512.
BAA16398; BAA16398; BAA16398.
GeneIDi946982.
KEGGiecj:JW2496.
eco:b2512.
PATRICi32120417. VBIEscCol129921_2611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75565.1.
AP009048 Genomic DNA. Translation: BAA16398.1.
PIRiG65027.
RefSeqiNP_417007.1. NC_000913.3.
WP_001177052.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YH3X-ray2.60A22-392[»]
2YMSX-ray2.10A62-191[»]
B113-186[»]
C248-322[»]
D247-320[»]
3P1LX-ray2.60A21-392[»]
3PRWX-ray1.80A21-392[»]
3Q7MX-ray1.65A21-392[»]
3Q7NX-ray1.77A21-392[»]
3Q7OX-ray2.09A21-392[»]
4PK1X-ray3.10A21-392[»]
4XGAX-ray2.15A20-392[»]
5AYWX-ray3.56B20-392[»]
5D0OX-ray2.90B1-392[»]
5LJOelectron microscopy4.90B22-392[»]
ProteinModelPortaliP77774.
SMRiP77774.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260587. 208 interactors.
DIPiDIP-12042N.
IntActiP77774. 8 interactors.
STRINGi511145.b2512.

Protein family/group databases

TCDBi1.B.33.1.3. the outer membrane protein insertion porin (bam complex) (ompip) family.

Proteomic databases

EPDiP77774.
PaxDbiP77774.
PRIDEiP77774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75565; AAC75565; b2512.
BAA16398; BAA16398; BAA16398.
GeneIDi946982.
KEGGiecj:JW2496.
eco:b2512.
PATRICi32120417. VBIEscCol129921_2611.

Organism-specific databases

EchoBASEiEB3960.
EcoGeneiEG14208. bamB.

Phylogenomic databases

eggNOGiENOG4105CNE. Bacteria.
COG1520. LUCA.
HOGENOMiHOG000260994.
InParanoidiP77774.
KOiK17713.
OMAiFTPTKVW.
PhylomeDBiP77774.

Enzyme and pathway databases

BioCyciEcoCyc:G7320-MONOMER.
ECOL316407:JW2496-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP77774.
PROiP77774.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
HAMAPiMF_00923. OM_assembly_BamB. 1 hit.
InterProiIPR017687. BamB.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
[Graphical view]
PfamiPF13360. PQQ_2. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 7 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
TIGRFAMsiTIGR03300. assembly_YfgL. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAMB_ECOLI
AccessioniPrimary (citable) accession number: P77774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.