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Protein

Recombination-promoting nuclease RpnB

Gene

rpnB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

A low activity DNA endonuclease yielding 3'-hydroxyl ends (Probable). Upon expression enhances RecA-independent DNA recombination 19-fold, concomitantly reducing viability by 98% and inducing DNA damage as measured by induction of the SOS repair response.1 Publication1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA recombination Source: UniProtKB

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA recombination
LigandMagnesium

Enzyme and pathway databases

BioCyciEcoCyc:G7197-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Recombination-promoting nuclease RpnB1 Publication (EC:3.1.21.-)
Gene namesi
Name:rpnB1 Publication
Synonyms:yfcI
Ordered Locus Names:b2305, JW2302
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14112. yfcI.

Pathology & Biotechi

Disruption phenotypei

A quadruple rpnA-rpnB-rpnC-rpnD deletion shows no change in basal RecA-independent recombination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691931 – 296Recombination-promoting nuclease RpnBAdd BLAST296

Proteomic databases

PaxDbiP77768.
PRIDEiP77768.

Interactioni

Protein-protein interaction databases

IntActiP77768. 3 interactors.
STRINGi316385.ECDH10B_2467.

Structurei

3D structure databases

ProteinModelPortaliP77768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Rpn/YhgA-like nuclease family.Curated

Phylogenomic databases

eggNOGiENOG4105DNE. Bacteria.
COG5464. LUCA.
HOGENOMiHOG000116866.
InParanoidiP77768.
PhylomeDBiP77768.

Family and domain databases

InterProiView protein in InterPro
IPR010106. RpnA.
IPR006842. Transposase_31.
PfamiView protein in Pfam
PF04754. Transposase_31. 1 hit.
TIGRFAMsiTIGR01784. T_den_put_tspse. 1 hit.

Sequencei

Sequence statusi: Complete.

P77768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISTTSTPH DAVFKSFLRH PDTARDFIDI HLPAPLRKLC DLTTLKLEPN
60 70 80 90 100
SFIDEDLRQY YSDLLWSVKT QEGVGYIYVV IEHQSKPEEL MAFRMMRYSI
110 120 130 140 150
AAMQNHLDAG YKELPLVLPM LFYHGCRSPY PYSLCWLDEF AEPAIARKIY
160 170 180 190 200
SSAFPLVDIT VVPDDEIMQH RKMALLELIQ KHIRQRDLLG LVDQIVSLLV
210 220 230 240 250
TGNTNDRQLK ALFNYVLQTG DAQRFRAFIG EIAERAPQEK EKLMTIADRL
260 270 280 290
REEGAMQGKH EEALRIAQEM LDRGLDRELV MMVTRLSPDD LIAQSH
Length:296
Mass (Da):34,179
Last modified:February 1, 1997 - v1
Checksum:i4FC384A2958F0D5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75365.1.
AP009048 Genomic DNA. Translation: BAA16142.1.
PIRiG65002.
RefSeqiNP_416808.1. NC_000913.3.
WP_000156149.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75365; AAC75365; b2305.
BAA16142; BAA16142; BAA16142.
GeneIDi946787.
KEGGiecj:JW2302.
eco:b2305.
PATRICifig|511145.12.peg.2400.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRPNB_ECOLI
AccessioniPrimary (citable) accession number: P77768
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 5, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families