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Protein

Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase

Gene

arnC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of 4-deoxy-4-formamido-L-arabinose from UDP to undecaprenyl phosphate. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.1 Publication

Catalytic activityi

UDP-4-deoxy-4-formamido-beta-L-arabinose + ditrans,octacis-undecaprenyl phosphate = UDP + 4-deoxy-4-formamido-alpha-L-arabinose ditrans,octacis-undecaprenyl phosphate.1 Publication

Pathwayi: 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate.1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC)
  2. Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD)
This subpathway is part of the pathway 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate, the pathway 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis and in Glycolipid biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processAntibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7167-MONOMER
MetaCyc:G7167-MONOMER
UniPathwayiUPA00030
UPA00036; UER00495

Protein family/group databases

CAZyiGT2 Glycosyltransferase Family 2
TCDBi4.D.1.1.13 the putative vectorial glycosyl polymerization (vgp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (EC:2.4.2.53)
Alternative name(s):
Polymyxin resistance protein PmrF
Undecaprenyl-phosphate Ara4FN transferase
Short name:
Ara4FN transferase
Gene namesi
Name:arnC
Synonyms:pmrF, yfbF
Ordered Locus Names:b2254, JW2248
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14090 arnC

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 235CytoplasmicSequence analysisAdd BLAST235
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Topological domaini257 – 269PeriplasmicSequence analysisAdd BLAST13
Transmembranei270 – 290HelicalSequence analysisAdd BLAST21
Topological domaini291 – 322CytoplasmicSequence analysisAdd BLAST32

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591951 – 322Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferaseAdd BLAST322

Proteomic databases

EPDiP77757
PaxDbiP77757
PRIDEiP77757

Expressioni

Inductioni

Induced by BasR.By similarity

Interactioni

Protein-protein interaction databases

BioGridi4260496, 315 interactors
IntActiP77757, 3 interactors
STRINGi316385.ECDH10B_2414

Structurei

3D structure databases

ProteinModelPortaliP77757
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CKK Bacteria
COG0463 LUCA
HOGENOMiHOG000283251
InParanoidiP77757
KOiK10012
OMAiDLVSGWK
PhylomeDBiP77757

Family and domain databases

Gene3Di3.90.550.10, 1 hit
HAMAPiMF_01164 ArnC_transfer, 1 hit
InterProiView protein in InterPro
IPR022857 ArnC_tfrase
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

P77757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEIHPVKKV SVVIPVYNEQ ESLPELIRRT TTACESLGKE YEILLIDDGS
60 70 80 90 100
SDNSAHMLVE ASQAENSHIV SILLNRNYGQ HSAIMAGFSH VTGDLIITLD
110 120 130 140 150
ADLQNPPEEI PRLVAKADEG YDVVGTVRQN RQDSWFRKTA SKMINRLIQR
160 170 180 190 200
TTGKAMGDYG CMLRAYRRHI VDAMLHCHER STFIPILANI FARRAIEIPV
210 220 230 240 250
HHAEREFGES KYSFMRLINL MYDLVTCLTT TPLRMLSLLG SIIAIGGFSI
260 270 280 290 300
AVLLVILRLT FGPQWAAEGV FMLFAVLFTF IGAQFIGMGL LGEYIGRIYT
310 320
DVRARPRYFV QQVIRPSSKE NE
Length:322
Mass (Da):36,339
Last modified:February 1, 1997 - v1
Checksum:i4E293C24969C2679
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ011863 Genomic DNA Translation: AAY34350.1
U00096 Genomic DNA Translation: AAC75314.1
AP009048 Genomic DNA Translation: BAA16077.1
PIRiD64996
RefSeqiNP_416757.1, NC_000913.3
WP_000461657.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75314; AAC75314; b2254
BAA16077; BAA16077; BAA16077
GeneIDi945275
KEGGiecj:JW2248
eco:b2254
PATRICifig|1411691.4.peg.4483

Similar proteinsi

Entry informationi

Entry nameiARNC_ECOLI
AccessioniPrimary (citable) accession number: P77757
Secondary accession number(s): P78179, Q4U4N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: April 25, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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