Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

7-cyano-7-deazaguanine synthase

Gene

queC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ0).By similarity1 Publication

Catalytic activityi

7-carboxy-7-carbaguanine + NH3 + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H2O.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi188 – 1881ZincBy similarity
Metal bindingi197 – 1971ZincBy similarity
Metal bindingi200 – 2001ZincBy similarity
Metal bindingi203 – 2031ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 1811ATPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G6245-MONOMER.
ECOL316407:JW0434-MONOMER.
MetaCyc:G6245-MONOMER.
UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
7-cyano-7-deazaguanine synthase (EC:6.3.4.20)
Alternative name(s):
7-cyano-7-carbaguanine synthase
PreQ(0) synthase
Queuosine biosynthesis protein QueC
Gene namesi
Name:queC
Synonyms:ybaX
Ordered Locus Names:b0444, JW0434
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13252. queC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2312317-cyano-7-deazaguanine synthasePRO_0000168631Add
BLAST

Proteomic databases

EPDiP77756.
PaxDbiP77756.
PRIDEiP77756.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tufAP0CE472EBI-560024,EBI-301077

Protein-protein interaction databases

BioGridi4262187. 7 interactions.
DIPiDIP-11311N.
IntActiP77756. 14 interactions.
MINTiMINT-1259911.
STRINGi511145.b0444.

Structurei

3D structure databases

ProteinModelPortaliP77756.
SMRiP77756. Positions 1-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the QueC family.Curated

Phylogenomic databases

eggNOGiENOG4105FD2. Bacteria.
COG0603. LUCA.
HOGENOMiHOG000110563.
InParanoidiP77756.
KOiK06920.
OMAiMAVSYAE.
PhylomeDBiP77756.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01633. QueC. 1 hit.
InterProiIPR018317. QueC.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF06508. QueC. 1 hit.
[Graphical view]
PIRSFiPIRSF006293. ExsB. 1 hit.
TIGRFAMsiTIGR00364. TIGR00364. 1 hit.

Sequencei

Sequence statusi: Complete.

P77756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRAVVVFSG GQDSTTCLVQ ALQQYDEVHC VTFDYGQRHR AEIDVARELA
60 70 80 90 100
LKLGARAHKV LDVTLLNELA VSSLTRDSIP VPDYEPEADG IPNTFVPGRN
110 120 130 140 150
ILFLTLAAIY AYQVKAEAVI TGVCETDFSG YPDCRDEFVK ALNHAVSLGM
160 170 180 190 200
AKDIRFETPL MWIDKAETWA LADYYGKLDL VRNETLTCYN GFKGDGCGHC
210 220 230
AACNLRANGL NHYLADKPTV MAAMKQKTGL R
Length:231
Mass (Da):25,514
Last modified:February 1, 1997 - v1
Checksum:i69E20ED03E6E3E34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82943 Genomic DNA. Translation: BAA11648.1.
U82664 Genomic DNA. Translation: AAB40200.1.
U00096 Genomic DNA. Translation: AAC73547.1.
AP009048 Genomic DNA. Translation: BAE76224.1.
PIRiD64774.
RefSeqiNP_414978.1. NC_000913.3.
WP_000817220.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73547; AAC73547; b0444.
BAE76224; BAE76224; BAE76224.
GeneIDi947034.
KEGGiecj:JW0434.
eco:b0444.
PATRICi32116041. VBIEscCol129921_0462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82943 Genomic DNA. Translation: BAA11648.1.
U82664 Genomic DNA. Translation: AAB40200.1.
U00096 Genomic DNA. Translation: AAC73547.1.
AP009048 Genomic DNA. Translation: BAE76224.1.
PIRiD64774.
RefSeqiNP_414978.1. NC_000913.3.
WP_000817220.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77756.
SMRiP77756. Positions 1-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262187. 7 interactions.
DIPiDIP-11311N.
IntActiP77756. 14 interactions.
MINTiMINT-1259911.
STRINGi511145.b0444.

Proteomic databases

EPDiP77756.
PaxDbiP77756.
PRIDEiP77756.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73547; AAC73547; b0444.
BAE76224; BAE76224; BAE76224.
GeneIDi947034.
KEGGiecj:JW0434.
eco:b0444.
PATRICi32116041. VBIEscCol129921_0462.

Organism-specific databases

EchoBASEiEB3041.
EcoGeneiEG13252. queC.

Phylogenomic databases

eggNOGiENOG4105FD2. Bacteria.
COG0603. LUCA.
HOGENOMiHOG000110563.
InParanoidiP77756.
KOiK06920.
OMAiMAVSYAE.
PhylomeDBiP77756.

Enzyme and pathway databases

UniPathwayiUPA00391.
BioCyciEcoCyc:G6245-MONOMER.
ECOL316407:JW0434-MONOMER.
MetaCyc:G6245-MONOMER.

Miscellaneous databases

PROiP77756.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01633. QueC. 1 hit.
InterProiIPR018317. QueC.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF06508. QueC. 1 hit.
[Graphical view]
PIRSFiPIRSF006293. ExsB. 1 hit.
TIGRFAMsiTIGR00364. TIGR00364. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEC_ECOLI
AccessioniPrimary (citable) accession number: P77756
Secondary accession number(s): Q2MBY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.