UniProtKB - P77754 (SPY_ECOLI)
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Protein
Periplasmic chaperone Spy
Gene
spy
Organism
Escherichia coli (strain K12)
Status
Functioni
An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface (PubMed:21317898, PubMed:24497545). Substrate protein folds while it is bound to chaperone (PubMed:26619265). Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates) (PubMed:24497545). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity (PubMed:21317898). Overexpression enhances the stability of otherwise unstable periplasmic proteins (PubMed:21317898).3 Publications
GO - Molecular functioni
- unfolded protein binding Source: EcoCyc
GO - Biological processi
- ATP-independent chaperone mediated protein folding Source: UniProtKB
- chaperone-mediated protein folding Source: EcoCyc
- response to organic cyclic compound Source: EcoCyc
Keywordsi
Molecular function | Chaperone |
Biological process | Stress response |
Enzyme and pathway databases
BioCyci | EcoCyc:G6939-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Periplasmic chaperone Spy1 PublicationAlternative name(s): Spheroplast protein Y1 Publication |
Gene namesi | Name:spy1 Publication Ordered Locus Names:b1743, JW1732 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG13490 spy |
Subcellular locationi
- Periplasm 3 Publications
GO - Cellular componenti
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
No observed growth phenotype (PubMed:9068658). Increased levels of degP and rpoH transcription; may induce the sigma factor E regulon (rpoE) (PubMed:10972835). Loss of about 50% of periplasmic alkaline phosphatase activity; very sensitive to tannic acid (PubMed:21317898).3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 48 | Q → R: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication | 1 | |
Mutagenesisi | 55 | L → P: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication | 1 | |
Mutagenesisi | 72 | Q → L: Increased chaperone activity, substrate affinity and chaperone flexibility, despite decreased Spy stability. 1 Publication | 1 | |
Mutagenesisi | 119 | H → L: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication | 1 | |
Mutagenesisi | 123 | Q → L: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication | 1 | |
Mutagenesisi | 138 | F → I or L: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 2 PublicationsAdd BLAST | 23 | |
ChainiPRO_0000022405 | 24 – 161 | Periplasmic chaperone SpyAdd BLAST | 138 |
Proteomic databases
EPDi | P77754 |
PaxDbi | P77754 |
PRIDEi | P77754 |
Expressioni
Developmental stagei
Produced abundantly in spheroplasts, not detected in intact cells.1 Publication
Inductioni
Induced by many sorts of periplasmic stress. Produced upon spheroplast formation, not induced by growth in 0.5 M sucrose or 0.35 M NaCl, nor by mutants that have a leaky periplasmic space (at protein level) (PubMed:9068658). Induction is partially dependent on the Cpx envelope stress response encoded by cpxA-cpxR; does not depend on sigma factor E (rpoE) (PubMed:10972835). Induction is also partially dependent on the Bae envelope stress response encoded by baeS-baeR; cpx and baeS effects are independent (PubMed:12354228). Overexpression of P pili protein PapG induces spy expression via both Cpx and Bae, overexpression of outer membrane protein NlpE induces spy via only Cpx whereas indole induces spy expression only via Bae (PubMed:12354228). Induced by 1 mM zinc (at protein level) (PubMed:9694902), via Bae, which also regulates ethanol induction of spy (PubMed:24999585). Induced by copper via Cpx (PubMed:24999585). Induced by tannic acid (to 25% of total periplasmic protein), butanol (to 20% total periplasmic protein) and ethanol (to 5% total periplasmic protein) (at protein level) (PubMed:21317898).6 Publications
Interactioni
Subunit structurei
Homodimer.By similarity
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
imm | Q03708 | 2 | EBI-1121716,EBI-1035025 | From Escherichia coli. |
GO - Molecular functioni
- unfolded protein binding Source: EcoCyc
Protein-protein interaction databases
BioGridi | 4262237147 interactors. |
DIPi | DIP-10914N |
IntActi | P77754 3 interactors. |
STRINGi | 316385.ECDH10B_1881 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 53 – 55 | Combined sources | 3 | |
Helixi | 59 – 69 | Combined sources | 11 | |
Helixi | 81 – 91 | Combined sources | 11 | |
Beta strandi | 92 – 95 | Combined sources | 4 | |
Helixi | 98 – 106 | Combined sources | 9 | |
Helixi | 109 – 127 | Combined sources | 19 | |
Helixi | 132 – 143 | Combined sources | 12 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5WNW | X-ray | 1.79 | A/B | 52-147 | [»] | |
5WO1 | X-ray | 1.87 | A/B | 52-147 | [»] | |
5WO2 | X-ray | 1.77 | A/B | 52-147 | [»] | |
5WO3 | X-ray | 1.87 | A/B | 52-147 | [»] | |
ProteinModelPortali | P77754 | |||||
SMRi | P77754 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Domaini
Has an elongated cradle shape.By similarity1 Publication
Sequence similaritiesi
Belongs to the CpxP/Spy family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG4108SPG Bacteria COG3678 LUCA |
HOGENOMi | HOG000285733 |
InParanoidi | P77754 |
OMAi | HDMMFKD |
PhylomeDBi | P77754 |
Family and domain databases
CDDi | cd09916 CpxP_like, 1 hit |
InterProi | View protein in InterPro IPR012899 LTXXQ |
Pfami | View protein in Pfam PF07813 LTXXQ, 1 hit |
PIRSFi | PIRSF034445 CpxP_Spy, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P77754-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRKLTALFVA STLALGAANL AHAADTTTAA PADAKPMMHH KGKFGPHQDM
60 70 80 90 100
MFKDLNLTDA QKQQIREIMK GQRDQMKRPP LEERRAMHDI IASDTFDKVK
110 120 130 140 150
AEAQIAKMEE QRKANMLAHM ETQNKIYNIL TPEQKKQFNA NFEKRLTERP
160
AAKGKMPATA E
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 | M → MM AA sequence (PubMed:9068658).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07714 Genomic DNA Translation: CAA68986.1 U00096 Genomic DNA Translation: AAC74813.1 AP009048 Genomic DNA Translation: BAA15531.1 |
PIRi | G64933 |
RefSeqi | NP_416257.1, NC_000913.3 WP_001228984.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74813; AAC74813; b1743 BAA15531; BAA15531; BAA15531 |
GeneIDi | 946253 |
KEGGi | ecj:JW1732 eco:b1743 |
PATRICi | fig|1411691.4.peg.513 |
Similar proteinsi
Entry informationi
Entry namei | SPY_ECOLI | |
Accessioni | P77754Primary (citable) accession number: P77754 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | March 28, 2018 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |