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P77754 (SPY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spheroplast protein Y
Gene names
Name:spy
Ordered Locus Names:b1743, JW1732
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could be involved in zinc resistance or another aspect of zinc metabolism.

Subcellular location

Periplasm.

Developmental stage

Produced abundantly in spheroplasts.

Sequence similarities

To E.coli CpxP.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processchaperone-mediated protein folding

Inferred from direct assay PubMed 21317898. Source: EcoCyc

   Cellular_componentouter membrane-bounded periplasmic space

Inferred from direct assay Ref.1. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.1 Ref.5
Chain24 – 161138Spheroplast protein Y
PRO_0000022405

Sequences

Sequence LengthMass (Da)Tools
P77754 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: B4733B967FC5F5BD

FASTA16118,199
        10         20         30         40         50         60 
MRKLTALFVA STLALGAANL AHAADTTTAA PADAKPMMHH KGKFGPHQDM MFKDLNLTDA 

        70         80         90        100        110        120 
QKQQIREIMK GQRDQMKRPP LEERRAMHDI IASDTFDKVK AEAQIAKMEE QRKANMLAHM 

       130        140        150        160 
ETQNKIYNIL TPEQKKQFNA NFEKRLTERP AAKGKMPATA E 

« Hide

References

« Hide 'large scale' references
[1]"A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells."
Hagenmaier S., Stierhof Y.-D., Henning U.
J. Bacteriol. 179:2073-2076(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-40.
Strain: K12.
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Identification of a novel transcription regulator from Proteus mirabilis, PMTR, revealed a possible role of YJAI protein in balancing zinc in Escherichia coli."
Noll M., Petrukhin K., Lutsenko S.
J. Biol. Chem. 273:21393-21401(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-38.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y07714 Genomic DNA. Translation: CAA68986.1.
U00096 Genomic DNA. Translation: AAC74813.1.
AP009048 Genomic DNA. Translation: BAA15531.1.
PIRG64933.
RefSeqNP_416257.1. NC_000913.3.
YP_490004.1. NC_007779.1.

3D structure databases

ProteinModelPortalP77754.
SMRP77754. Positions 52-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP77754. 2 interactions.
STRING511145.b1743.

Proteomic databases

PaxDbP77754.
PRIDEP77754.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74813; AAC74813; b1743.
BAA15531; BAA15531; BAA15531.
GeneID12933243.
946253.
KEGGecj:Y75_p1718.
eco:b1743.
PATRIC32118795. VBIEscCol129921_1815.

Organism-specific databases

EchoBASEEB3263.
EcoGeneEG13490. spy.

Phylogenomic databases

eggNOGCOG3678.
HOGENOMHOG000285733.
OMAKMYNLLT.
OrthoDBEOG63Z790.
PhylomeDBP77754.

Enzyme and pathway databases

BioCycEcoCyc:G6939-MONOMER.
ECOL316407:JW1732-MONOMER.

Gene expression databases

GenevestigatorP77754.

Family and domain databases

InterProIPR012899. LTXXQ.
[Graphical view]
PfamPF07813. LTXXQ. 1 hit.
[Graphical view]
PIRSFPIRSF034445. CpxP_Spy. 1 hit.
ProtoNetSearch...

Other

PROP77754.

Entry information

Entry nameSPY_ECOLI
AccessionPrimary (citable) accession number: P77754
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene