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Protein

Periplasmic chaperone Spy

Gene

spy

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface (PubMed:21317898, PubMed:24497545). Substrate protein folds while it is bound to chaperone (PubMed:26619265). Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates) (PubMed:24497545). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity (PubMed:21317898). Overexpression enhances the stability of otherwise unstable periplasmic proteins (PubMed:21317898).3 Publications

GO - Biological processi

  • ATP-independent chaperone mediated protein folding Source: UniProtKB
  • chaperone-mediated protein folding Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:G6939-MONOMER.
ECOL316407:JW1732-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic chaperone Spy1 Publication
Alternative name(s):
Spheroplast protein Y1 Publication
Gene namesi
Name:spy1 Publication
Ordered Locus Names:b1743, JW1732
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13490. spy.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

No observed growth phenotype (PubMed:9068658). Increased levels of degP and rpoH transcription; may induce the sigma factor E regulon (rpoE) (PubMed:10972835). Loss of about 50% of periplasmic alkaline phosphatase activity; very sensitive to tannic acid (PubMed:21317898).3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481Q → R: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication
Mutagenesisi55 – 551L → P: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication
Mutagenesisi72 – 721Q → L: Increased chaperone activity, substrate affinity and chaperone flexibility, despite decreased Spy stability. 1 Publication
Mutagenesisi119 – 1191H → L: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication
Mutagenesisi123 – 1231Q → L: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication
Mutagenesisi138 – 1381F → I or L: Increased chaperone activity, substrate affinity and chaperone flexibility. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23232 PublicationsAdd
BLAST
Chaini24 – 161138Periplasmic chaperone SpyPRO_0000022405Add
BLAST

Proteomic databases

EPDiP77754.
PaxDbiP77754.
PRIDEiP77754.

Expressioni

Developmental stagei

Produced abundantly in spheroplasts, not detected in intact cells.1 Publication

Inductioni

Induced by many sorts of periplasmic stress. Produced upon spheroplast formation, not induced by growth in 0.5 M sucrose or 0.35 M NaCl, nor by mutants that have a leaky periplasmic space (at protein level) (PubMed:9068658). Induction is partially dependent on the Cpx envelope stress response encoded by cpxA-cpxR; does not depend on sigma factor E (rpoE) (PubMed:10972835). Induction is also partially dependent on the Bae envelope stress response encoded by baeS-baeR; cpx and baeS effects are independent (PubMed:12354228). Overexpression of P pili protein PapG induces spy expression via both Cpx and Bae, overexpression of outer membrane protein NlpE induces spy via only Cpx whereas indole induces spy expression only via Bae (PubMed:12354228). Induced by 1 mM zinc (at protein level) (PubMed:9694902), via Bae, which also regulates ethanol induction of spy (PubMed:24999585). Induced by copper via Cpx (PubMed:24999585). Induced by tannic acid (to 25% of total periplasmic protein), butanol (to 20% total periplasmic protein) and ethanol (to 5% total periplasmic protein) (at protein level) (PubMed:21317898).6 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262237. 141 interactions.
IntActiP77754. 2 interactions.
STRINGi511145.b1743.

Structurei

3D structure databases

ProteinModelPortaliP77754.
SMRiP77754. Positions 52-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Has an elongated cradle shape.By similarity1 Publication

Sequence similaritiesi

Belongs to the CpxP/Spy family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108SPG. Bacteria.
COG3678. LUCA.
HOGENOMiHOG000285733.
InParanoidiP77754.
OMAiKMYNLLT.
OrthoDBiEOG63Z790.
PhylomeDBiP77754.

Family and domain databases

InterProiIPR012899. LTXXQ.
[Graphical view]
PfamiPF07813. LTXXQ. 1 hit.
[Graphical view]
PIRSFiPIRSF034445. CpxP_Spy. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P77754-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKLTALFVA STLALGAANL AHAADTTTAA PADAKPMMHH KGKFGPHQDM
60 70 80 90 100
MFKDLNLTDA QKQQIREIMK GQRDQMKRPP LEERRAMHDI IASDTFDKVK
110 120 130 140 150
AEAQIAKMEE QRKANMLAHM ETQNKIYNIL TPEQKKQFNA NFEKRLTERP
160
AAKGKMPATA E
Length:161
Mass (Da):18,199
Last modified:February 1, 1997 - v1
Checksum:iB4733B967FC5F5BD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381M → MM AA sequence (PubMed:9068658).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07714 Genomic DNA. Translation: CAA68986.1.
U00096 Genomic DNA. Translation: AAC74813.1.
AP009048 Genomic DNA. Translation: BAA15531.1.
PIRiG64933.
RefSeqiNP_416257.1. NC_000913.3.
WP_001228984.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74813; AAC74813; b1743.
BAA15531; BAA15531; BAA15531.
GeneIDi946253.
KEGGiecj:JW1732.
eco:b1743.
PATRICi32118795. VBIEscCol129921_1815.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07714 Genomic DNA. Translation: CAA68986.1.
U00096 Genomic DNA. Translation: AAC74813.1.
AP009048 Genomic DNA. Translation: BAA15531.1.
PIRiG64933.
RefSeqiNP_416257.1. NC_000913.3.
WP_001228984.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77754.
SMRiP77754. Positions 52-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262237. 141 interactions.
IntActiP77754. 2 interactions.
STRINGi511145.b1743.

Proteomic databases

EPDiP77754.
PaxDbiP77754.
PRIDEiP77754.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74813; AAC74813; b1743.
BAA15531; BAA15531; BAA15531.
GeneIDi946253.
KEGGiecj:JW1732.
eco:b1743.
PATRICi32118795. VBIEscCol129921_1815.

Organism-specific databases

EchoBASEiEB3263.
EcoGeneiEG13490. spy.

Phylogenomic databases

eggNOGiENOG4108SPG. Bacteria.
COG3678. LUCA.
HOGENOMiHOG000285733.
InParanoidiP77754.
OMAiKMYNLLT.
OrthoDBiEOG63Z790.
PhylomeDBiP77754.

Enzyme and pathway databases

BioCyciEcoCyc:G6939-MONOMER.
ECOL316407:JW1732-MONOMER.

Miscellaneous databases

PROiP77754.

Family and domain databases

InterProiIPR012899. LTXXQ.
[Graphical view]
PfamiPF07813. LTXXQ. 1 hit.
[Graphical view]
PIRSFiPIRSF034445. CpxP_Spy. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells."
    Hagenmaier S., Stierhof Y.-D., Henning U.
    J. Bacteriol. 179:2073-2076(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-40, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification of a novel transcription regulator from Proteus mirabilis, PMTR, revealed a possible role of YJAI protein in balancing zinc in Escherichia coli."
    Noll M., Petrukhin K., Lutsenko S.
    J. Biol. Chem. 273:21393-21401(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-38, SUBCELLULAR LOCATION, INDUCTION BY ZINC.
  6. "Tethering of CpxP to the inner membrane prevents spheroplast induction of the cpx envelope stress response."
    Raivio T.L., Laird M.W., Joly J.C., Silhavy T.J.
    Mol. Microbiol. 37:1186-1197(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY CPX ENVELOPE STRESS RESPONSE, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100.
  7. "A third envelope stress signal transduction pathway in Escherichia coli."
    Raffa R.G., Raivio T.L.
    Mol. Microbiol. 45:1599-1611(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY BOTH BAE AND CXP ENVELOPE STRESS RESPONSES.
    Strain: K12 / MC4100.
  8. Cited for: FUNCTION, SUBSTRATE-BINDING, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.
  9. Cited for: FUNCTION, SUBSTRATE-BINDING, MUTAGENESIS OF GLN-48; LEU-55; GLN-72; HIS-119; GLN-123 AND PHE-138.
    Strain: K12 / MG1655 / ATCC 47076.
  10. "Genetic regulation of spy gene expression in Escherichia coli in the presence of protein unfolding agent ethanol."
    Srivastava S.K., Lambadi P.R., Ghosh T., Pathania R., Navani N.K.
    Gene 548:142-148(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / BW25113.
  11. "Substrate protein folds while it is bound to the ATP-independent chaperone Spy."
    Stull F., Koldewey P., Humes J.R., Radford S.E., Bardwell J.C.
    Nat. Struct. Mol. Biol. 23:53-58(2016) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REACTION MECHANISM.

Entry informationi

Entry nameiSPY_ECOLI
AccessioniPrimary (citable) accession number: P77754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 16, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.