P77754 (SPY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spheroplast protein Y

Gene names

Name:	spy
Ordered Locus Names:	b1743, JW1732

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could be involved in zinc resistance or another aspect of zinc metabolism.
Subcellular location	Periplasm.
Developmental stage	Produced abundantly in spheroplasts.
Sequence similarities	To E.coli CpxP.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chaperone-mediated protein folding Inferred from direct assay PubMed 21317898. Source: EcoCyc
Cellular_component	outer membrane-bounded periplasmic space Inferred from direct assay Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1 Ref.5
	Chain	24 – 161	138	Spheroplast protein Y		PRO_0000022405

Sequences

	Sequence		Length	Mass (Da)
	P77754 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B4733B967FC5F5BD	FASTA	161	18,199
	10 20 30 40 50 60 MRKLTALFVA STLALGAANL AHAADTTTAA PADAKPMMHH KGKFGPHQDM MFKDLNLTDA 70 80 90 100 110 120 QKQQIREIMK GQRDQMKRPP LEERRAMHDI IASDTFDKVK AEAQIAKMEE QRKANMLAHM 130 140 150 160 ETQNKIYNIL TPEQKKQFNA NFEKRLTERP AAKGKMPATA E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells." Hagenmaier S., Stierhof Y.-D., Henning U. J. Bacteriol. 179:2073-2076(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-40. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Identification of a novel transcription regulator from Proteus mirabilis, PMTR, revealed a possible role of YJAI protein in balancing zinc in Escherichia coli." Noll M., Petrukhin K., Lutsenko S. J. Biol. Chem. 273:21393-21401(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-38.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07714 Genomic DNA. Translation: CAA68986.1. U00096 Genomic DNA. Translation: AAC74813.1. AP009048 Genomic DNA. Translation: BAA15531.1.
PIR	G64933.
RefSeq	NP_416257.1. NC_000913.3. YP_490004.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P77754.
SMR	P77754. Positions 52-147.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
IntAct	P77754. 2 interactions.
STRING	511145.b1743.
Proteomic databases
PaxDb	P77754.
PRIDE	P77754.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74813; AAC74813; b1743. BAA15531; BAA15531; BAA15531.
GeneID	12933243. 946253.
KEGG	ecj:Y75_p1718. eco:b1743.
PATRIC	32118795. VBIEscCol129921_1815.
Organism-specific databases
EchoBASE	EB3263.
EcoGene	EG13490. spy.
Phylogenomic databases
eggNOG	COG3678.
HOGENOM	HOG000285733.
OMA	KMYNLLT.
OrthoDB	EOG63Z790.
PhylomeDB	P77754.
Enzyme and pathway databases
BioCyc	EcoCyc:G6939-MONOMER. ECOL316407:JW1732-MONOMER.
Gene expression databases
Genevestigator	P77754.
Family and domain databases
InterPro	IPR012899. LTXXQ. [Graphical view]
Pfam	PF07813. LTXXQ. 1 hit. [Graphical view]
PIRSF	PIRSF034445. CpxP_Spy. 1 hit.
ProtoNet	Search...
Other
PRO	P77754.

Entry information

Entry name

SPY_ECOLI

Accession

Primary (citable) accession number: P77754

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program