Reviewed, UniProtKB/Swiss-Prot P77713 (YAGH_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative beta-xylosidase
    EC=3.2.1.37
Alternative name(s):
    1,4-beta-D-xylan xylohydrolase
    Xylan 1,4-beta-xylosidase

Gene names

Name:	yagH
Ordered Locus Names:	b0271, JW0264

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.
Sequence similarities	Belongs to the glycosyl hydrolase 43 family.

Ontologies

Keywords
Biological process	Xylan degradation
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	xylan 1,4-beta-xylosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Putative beta-xylosidase		PRO_0000057697

Sequences

	Sequence		Length	Mass (Da)
	P77713-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 60D3F038215CD216	FASTA	536	60,825
	10 20 30 40 50 60 MEITNPILTG FNPDPSLCRQ GEDYYIATST FEWFPGVRIY HSRDLKNWSL VSTPLDRVSM 70 80 90 100 110 120 LDMKGNPDSG GIWAPCLSYA DGKFWLLYTD VKIVDSPWKN GRNFLVTAPS IEGPWSEPIP 130 140 150 160 170 180 MGNGGFDPSL FHDDDGRKYY IYRPWGPRHH SNPHNTIVLQ AFDPQTGTLS PERKTLFTGT 190 200 210 220 230 240 PLCYTEGAHL YRHAGWYYLM AAEGGTSYEH AVVVLRSKNI DGPYELHPDV TMMTSWHLPE 250 260 270 280 290 300 NPLQKSGHGS LLQTHTGEWY MAYLTSRPLR LPGVPLLASG GRGYCPLGRE TGIARIEWRD 310 320 330 340 350 360 GWPYVEGGKH AQLTVKGPQV AEQPAAVPGN WRDDFDASSL DPELQTLRIP FDDTLGSLTA 370 380 390 400 410 420 RPGFLRLYGN DSLNSTFTQS TVARRWQHFA FRAETRMEFS PVHFQQSAGL TCYYNSKNWS 430 440 450 460 470 480 YCFVDYEEGQ GRTIKVIQLD HNVPSWPLHE QPIPVPEHAE SVWLRVDVDT LVYRYSYSFD 490 500 510 520 530 GETWHTVPVT YEAWKLSDDY IGGRGFFTGA FVGLHCEDIS GDGCYADFDY FTYEPV

References

[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U70214 Genomic DNA. Translation: AAB08692.1. U00096 Genomic DNA. Translation: AAC73374.1. AP009048 Genomic DNA. Translation: BAE76055.1.
PIR	G64752.
RefSeq	AP_000925.1. NP_414805.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P77713.
Protein family/group databases
CAZy	GH43. Glycoside Hydrolase Family 43.
Genome annotation databases
GeneID	944949.
GenomeReviews	Gene locus JW0264 in contig AP009048_GR. Gene locus b0271 in contig U00096_GR.
KEGG	ecj:JW0264. eco:b0271.
Organism-specific databases
EchoBASE	EB3131.
EcoGene	EG13347. yagH.
CMR	Search...
Phylogenomic databases
HOGENOM	P77713.
OMA	GPYEVHP.
Enzyme and pathway databases
BioCyc	EcoCyc:G6143-MON.
Gene expression databases
Genevestigator	P77713.
Family and domain databases
InterPro	IPR013320. ConA-like_subgrp. IPR006710. Glyco_hydro_43. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
PANTHER	PTHR22925. Glyco_hydro_43. 1 hit.
Pfam	PF04616. Glyco_hydro_43. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAGH_ECOLI

Accession

Primary (citable) accession number: P77713
Secondary accession number(s): Q2MCF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)