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Protein

Putative beta-xylosidase

Gene

yagH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei14Proton acceptorBy similarity1
Sitei127Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrateBy similarity1
Active sitei186Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Multifunctional enzyme
Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

BioCyciEcoCyc:G6143-MONOMER

Protein family/group databases

CAZyiGH43 Glycoside Hydrolase Family 43

Names & Taxonomyi

Protein namesi
Recommended name:
Putative beta-xylosidase (EC:3.2.1.37)
Alternative name(s):
1,4-beta-D-xylan xylohydrolase
Xylan 1,4-beta-xylosidase
Gene namesi
Name:yagH
Ordered Locus Names:b0271, JW0264
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13347 yagH

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000576971 – 536Putative beta-xylosidaseAdd BLAST536

Proteomic databases

PaxDbiP77713
PRIDEiP77713

Interactioni

Protein-protein interaction databases

BioGridi4261986, 9 interactors
IntActiP77713, 5 interactors
STRINGi316385.ECDH10B_0258

Structurei

3D structure databases

ProteinModelPortaliP77713
SMRiP77713
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 43 family.Curated

Phylogenomic databases

eggNOGiENOG4105DSG Bacteria
COG3507 LUCA
HOGENOMiHOG000112982
InParanoidiP77713
KOiK01198
OMAiDERQFAG
PhylomeDBiP77713

Family and domain databases

Gene3Di2.115.10.20, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR009784 DUF1349
IPR006710 Glyco_hydro_43
IPR023296 Glyco_hydro_beta-prop_sf
PfamiView protein in Pfam
PF07081 DUF1349, 1 hit
PF04616 Glyco_hydro_43, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF75005 SSF75005, 1 hit

Sequencei

Sequence statusi: Complete.

P77713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEITNPILTG FNPDPSLCRQ GEDYYIATST FEWFPGVRIY HSRDLKNWSL
60 70 80 90 100
VSTPLDRVSM LDMKGNPDSG GIWAPCLSYA DGKFWLLYTD VKIVDSPWKN
110 120 130 140 150
GRNFLVTAPS IEGPWSEPIP MGNGGFDPSL FHDDDGRKYY IYRPWGPRHH
160 170 180 190 200
SNPHNTIVLQ AFDPQTGTLS PERKTLFTGT PLCYTEGAHL YRHAGWYYLM
210 220 230 240 250
AAEGGTSYEH AVVVLRSKNI DGPYELHPDV TMMTSWHLPE NPLQKSGHGS
260 270 280 290 300
LLQTHTGEWY MAYLTSRPLR LPGVPLLASG GRGYCPLGRE TGIARIEWRD
310 320 330 340 350
GWPYVEGGKH AQLTVKGPQV AEQPAAVPGN WRDDFDASSL DPELQTLRIP
360 370 380 390 400
FDDTLGSLTA RPGFLRLYGN DSLNSTFTQS TVARRWQHFA FRAETRMEFS
410 420 430 440 450
PVHFQQSAGL TCYYNSKNWS YCFVDYEEGQ GRTIKVIQLD HNVPSWPLHE
460 470 480 490 500
QPIPVPEHAE SVWLRVDVDT LVYRYSYSFD GETWHTVPVT YEAWKLSDDY
510 520 530
IGGRGFFTGA FVGLHCEDIS GDGCYADFDY FTYEPV
Length:536
Mass (Da):60,825
Last modified:February 1, 1997 - v1
Checksum:i60D3F038215CD216
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA Translation: AAB08692.1
U00096 Genomic DNA Translation: AAC73374.1
AP009048 Genomic DNA Translation: BAE76055.1
PIRiG64752
RefSeqiNP_414805.1, NC_000913.3
WP_000406871.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73374; AAC73374; b0271
BAE76055; BAE76055; BAE76055
GeneIDi944949
KEGGiecj:JW0264
eco:b0271
PATRICifig|1411691.4.peg.2009

Similar proteinsi

Entry informationi

Entry nameiYAGH_ECOLI
AccessioniPrimary (citable) accession number: P77713
Secondary accession number(s): Q2MCF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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