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Protein

Long-chain acyl-CoA thioesterase FadM

Gene

fadM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Long-chain acyl-CoA thioesterase with a preference for 3,5-tetradecadienoyl-CoA. Could be involved in beta-oxidation of fatty acids.1 Publication

Kineticsi

  1. KM=3.0 µM for 3,5-cis-tetradecadienoyl-CoA1 Publication
  2. KM=10.8 µM for 3,5-cis-dodecadienoyl-CoA1 Publication
  3. KM=5.8 µM for 3-hydroxytetradecanoyl-CoA1 Publication
  4. KM=6.0 µM for palmitoyl-CoA1 Publication
  5. KM=6.3 µM for myristoyl-CoA1 Publication
  6. KM=13.0 µM for lauroyl-CoA1 Publication
  1. Vmax=101.3 µmol/min/mg enzyme with 3,5-cis-tetradecadienoyl-CoA as substrate1 Publication
  2. Vmax=92.3 µmol/min/mg enzyme with 3,5-cis-dodecadienoyl-CoA as substrate1 Publication
  3. Vmax=45.5 µmol/min/mg enzyme with 3-hydroxytetradecanoyl-CoA as substrate1 Publication
  4. Vmax=43.5 µmol/min/mg enzyme with palmitoyl-CoA as substrate1 Publication
  5. Vmax=26.4 µmol/min/mg enzyme with myristoyl-CoA as substrate1 Publication
  6. Vmax=6.9 µmol/min/mg enzyme with lauroyl-CoA as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei13 – 131Sequence analysis

GO - Molecular functioni

  • acyl-CoA hydrolase activity Source: EcoCyc

GO - Biological processi

  • fatty acid beta-oxidation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6244-MONOMER.
ECOL316407:JW0433-MONOMER.
MetaCyc:G6244-MONOMER.
BRENDAi3.1.2.20. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain acyl-CoA thioesterase FadM (EC:3.1.2.-)
Alternative name(s):
Acyl-CoA thioester hydrolase
Thioesterase 3
Gene namesi
Name:fadM
Synonyms:tesC, ybaW
Ordered Locus Names:b0443, JW0433
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13251. fadM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 132132Long-chain acyl-CoA thioesterase FadMPRO_0000168630Add
BLAST

Proteomic databases

PaxDbiP77712.

Expressioni

Inductioni

Up-regulated by growth on oleic acid. Repressed by FadR and by the cyclic AMP receptor protein-cAMP (CRP-cAMP) complex.2 Publications

Interactioni

Subunit structurei

Homotetramer.Curated

Protein-protein interaction databases

BioGridi4259836. 162 interactions.
IntActiP77712. 4 interactions.
STRINGi511145.b0443.

Structurei

Secondary structure

132
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 66Combined sources
Helixi9 – 113Combined sources
Beta strandi16 – 183Combined sources
Helixi20 – 3617Combined sources
Helixi39 – 468Combined sources
Beta strandi49 – 6012Combined sources
Beta strandi69 – 8012Combined sources
Beta strandi83 – 9210Combined sources
Turni93 – 964Combined sources
Beta strandi97 – 11014Combined sources
Turni111 – 1133Combined sources
Beta strandi115 – 1173Combined sources
Turni121 – 1244Combined sources
Helixi125 – 1317Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NJKX-ray1.90A/B/C/D1-132[»]
ProteinModelPortaliP77712.
SMRiP77712. Positions 1-132.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77712.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108TGP. Bacteria.
COG0824. LUCA.
HOGENOMiHOG000004132.
InParanoidiP77712.
KOiK12500.
OMAiANININY.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
InterProiIPR029069. HotDog_dom.
IPR006684. YbgC/YbaW.
[Graphical view]
PIRSFiPIRSF003230. YbgC. 1 hit.
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR00051. TIGR00051. 1 hit.

Sequencei

Sequence statusi: Complete.

P77712-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTQIKVRGY HLDVYQHVNN ARYLEFLEEA RWDGLENSDS FQWMTAHNIA
60 70 80 90 100
FVVVNININY RRPAVLSDLL TITSQLQQLN GKSGILSQVI TLEPEGQVVA
110 120 130
DALITFVCID LKTQKALALE GELREKLEQM VK
Length:132
Mass (Da):15,088
Last modified:February 1, 1997 - v1
Checksum:iEBB3539149A37383
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82943 Genomic DNA. Translation: BAA11647.1.
U82664 Genomic DNA. Translation: AAB40199.1.
U00096 Genomic DNA. Translation: AAC73546.1.
AP009048 Genomic DNA. Translation: BAE76223.1.
PIRiC64774.
RefSeqiNP_414977.1. NC_000913.3.
WP_001194534.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73546; AAC73546; b0443.
BAE76223; BAE76223; BAE76223.
GeneIDi945812.
KEGGiecj:JW0433.
eco:b0443.
PATRICi32116039. VBIEscCol129921_0461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82943 Genomic DNA. Translation: BAA11647.1.
U82664 Genomic DNA. Translation: AAB40199.1.
U00096 Genomic DNA. Translation: AAC73546.1.
AP009048 Genomic DNA. Translation: BAE76223.1.
PIRiC64774.
RefSeqiNP_414977.1. NC_000913.3.
WP_001194534.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NJKX-ray1.90A/B/C/D1-132[»]
ProteinModelPortaliP77712.
SMRiP77712. Positions 1-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259836. 162 interactions.
IntActiP77712. 4 interactions.
STRINGi511145.b0443.

Proteomic databases

PaxDbiP77712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73546; AAC73546; b0443.
BAE76223; BAE76223; BAE76223.
GeneIDi945812.
KEGGiecj:JW0433.
eco:b0443.
PATRICi32116039. VBIEscCol129921_0461.

Organism-specific databases

EchoBASEiEB3040.
EcoGeneiEG13251. fadM.

Phylogenomic databases

eggNOGiENOG4108TGP. Bacteria.
COG0824. LUCA.
HOGENOMiHOG000004132.
InParanoidiP77712.
KOiK12500.
OMAiANININY.

Enzyme and pathway databases

BioCyciEcoCyc:G6244-MONOMER.
ECOL316407:JW0433-MONOMER.
MetaCyc:G6244-MONOMER.
BRENDAi3.1.2.20. 2026.

Miscellaneous databases

EvolutionaryTraceiP77712.
PROiP77712.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
InterProiIPR029069. HotDog_dom.
IPR006684. YbgC/YbaW.
[Graphical view]
PIRSFiPIRSF003230. YbgC. 1 hit.
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR00051. TIGR00051. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFADM_ECOLI
AccessioniPrimary (citable) accession number: P77712
Secondary accession number(s): Q2MBY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.