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Protein

UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase

Gene

arnB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.1 Publication

Catalytic activityi

UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate = UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate.1 Publication

Cofactori

Pathwayi: UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate.1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Bifunctional polymyxin resistance protein ArnA (arnA)
  2. UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB)
  3. Bifunctional polymyxin resistance protein ArnA (arnA)
This subpathway is part of the pathway UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate, the pathway UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • pyridoxal phosphate binding Source: EcoCyc
  • transaminase activity Source: EcoCyc
  • undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:G7166-MONOMER.
ECOL316407:JW5372-MONOMER.
MetaCyc:G7166-MONOMER.
BRENDAi2.6.1.87. 2026.
UniPathwayiUPA00030.
UPA00032; UER00493.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (EC:2.6.1.871 Publication)
Alternative name(s):
Polymyxin resistance protein PmrH
UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase
Short name:
UDP-Ara4O aminotransferase
UDP-4-amino-4-deoxy-L-arabinose aminotransferase
Gene namesi
Name:arnB
Synonyms:pmrH, yfbE
Ordered Locus Names:b2253, JW5372
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14089. arnB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001100181 – 385UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferaseAdd BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

EPDiP77690.
PaxDbiP77690.
PRIDEiP77690.

Expressioni

Inductioni

Induced by BasR.By similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4259180. 233 interactors.
STRINGi511145.b2253.

Structurei

3D structure databases

ProteinModelPortaliP77690.
SMRiP77690.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DegT/DnrJ/EryC1 family. ArnB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CF4. Bacteria.
COG0399. LUCA.
HOGENOMiHOG000230163.
InParanoidiP77690.
KOiK07806.
OMAiSEWNSAR.
PhylomeDBiP77690.

Family and domain databases

CDDicd00616. AHBA_syn. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01167. ArnB_transfer. 1 hit.
InterProiIPR022850. ArnB_NH2Trfase.
IPR000653. DegT/StrS_aminotransferase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
[Graphical view]
PIRSFiPIRSF000390. PLP_StrS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

P77690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGKAMSEF LPFSRPAMGV EELAAVKEVL ESGWITTGPK NQALEQAFCQ
60 70 80 90 100
LTGNQHAIAV SSATAGMHIT LMALKIGKGD EVITPSLTWV STLNMISLLG
110 120 130 140 150
ATPVMVDVDR DTLMVTPEAI ESAITPRTKA IIPVHYAGAP ADIDAIRAIG
160 170 180 190 200
ERYGIAVIED AAHAVGTYYK GRHIGAKGTA IFSFHAIKNI TCAEGGLIVT
210 220 230 240 250
DNENLARQLR MLKFHGLGVD AYDRQTWGRA PQAEVLTPGY KYNLTDINAA
260 270 280 290 300
IALTQLVKLE HLNTRRREIA QQYQQALAAL PFQPLSLPAW PHVHAWHLFI
310 320 330 340 350
IRVDEQRCGI SRDALMEALK ERGIGTGLHF RAAHTQKYYR ERFPTLSLPN
360 370 380
TEWNSERICS LPLFPDMTTA DADHVITALQ QLAGQ
Length:385
Mass (Da):42,238
Last modified:November 30, 2016 - v3
Checksum:i2017E3C950FFF4B8
GO

Sequence cautioni

The sequence AAC75313 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF527386 Genomic DNA. Translation: AAM92146.1.
U00096 Genomic DNA. Translation: AAC75313.3. Different initiation.
AP009048 Genomic DNA. Translation: BAA16076.2.
PIRiC64996.
RefSeqiNP_416756.5. NC_000913.3.
WP_001295286.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75313; AAC75313; b2253.
BAA16076; BAA16076; BAA16076.
GeneIDi947375.
KEGGiecj:JW5372.
eco:b2253.
PATRICi32119871. VBIEscCol129921_2345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF527386 Genomic DNA. Translation: AAM92146.1.
U00096 Genomic DNA. Translation: AAC75313.3. Different initiation.
AP009048 Genomic DNA. Translation: BAA16076.2.
PIRiC64996.
RefSeqiNP_416756.5. NC_000913.3.
WP_001295286.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77690.
SMRiP77690.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259180. 233 interactors.
STRINGi511145.b2253.

Proteomic databases

EPDiP77690.
PaxDbiP77690.
PRIDEiP77690.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75313; AAC75313; b2253.
BAA16076; BAA16076; BAA16076.
GeneIDi947375.
KEGGiecj:JW5372.
eco:b2253.
PATRICi32119871. VBIEscCol129921_2345.

Organism-specific databases

EchoBASEiEB3842.
EcoGeneiEG14089. arnB.

Phylogenomic databases

eggNOGiENOG4105CF4. Bacteria.
COG0399. LUCA.
HOGENOMiHOG000230163.
InParanoidiP77690.
KOiK07806.
OMAiSEWNSAR.
PhylomeDBiP77690.

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00032; UER00493.
BioCyciEcoCyc:G7166-MONOMER.
ECOL316407:JW5372-MONOMER.
MetaCyc:G7166-MONOMER.
BRENDAi2.6.1.87. 2026.

Miscellaneous databases

PROiP77690.

Family and domain databases

CDDicd00616. AHBA_syn. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01167. ArnB_transfer. 1 hit.
InterProiIPR022850. ArnB_NH2Trfase.
IPR000653. DegT/StrS_aminotransferase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
[Graphical view]
PIRSFiPIRSF000390. PLP_StrS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARNB_ECOLI
AccessioniPrimary (citable) accession number: P77690
Secondary accession number(s): Q8KRQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 30, 2016
Last modified: November 30, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.