Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase

Gene

arnB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.1 Publication

Catalytic activityi

UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate = UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate.1 Publication

Cofactori

Pathwayi: UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate.1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Bifunctional polymyxin resistance protein ArnA (arnA)
  2. UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB)
  3. Bifunctional polymyxin resistance protein ArnA (arnA)
This subpathway is part of the pathway UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate, the pathway UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • pyridoxal phosphate binding Source: EcoCyc
  • UDP-4-amino-4-deoxy-L-arabinose aminotransferase Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAntibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:G7166-MONOMER
MetaCyc:G7166-MONOMER
BRENDAi2.6.1.87 2026
UniPathwayiUPA00030
UPA00032; UER00493

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (EC:2.6.1.871 Publication)
Alternative name(s):
Polymyxin resistance protein PmrH
UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase
Short name:
UDP-Ara4O aminotransferase
UDP-4-amino-4-deoxy-L-arabinose aminotransferase
Gene namesi
Name:arnB
Synonyms:pmrH, yfbE
Ordered Locus Names:b2253, JW5372
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14089 arnB

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001100181 – 385UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferaseAdd BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

EPDiP77690
PaxDbiP77690
PRIDEiP77690

Expressioni

Inductioni

Induced by BasR.By similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4259180238 interactors.
STRINGi316385.ECDH10B_2413

Structurei

3D structure databases

ProteinModelPortaliP77690
SMRiP77690
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DegT/DnrJ/EryC1 family. ArnB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CF4 Bacteria
COG0399 LUCA
HOGENOMiHOG000230163
InParanoidiP77690
KOiK07806
PhylomeDBiP77690

Family and domain databases

CDDicd00616 AHBA_syn, 1 hit
Gene3Di3.40.640.101 hit
3.90.1150.101 hit
HAMAPiMF_01167 ArnB_transfer, 1 hit
InterProiView protein in InterPro
IPR022850 ArnB_NH2Trfase
IPR000653 DegT/StrS_aminotransferase
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR30244 PTHR30244, 1 hit
PfamiView protein in Pfam
PF01041 DegT_DnrJ_EryC1, 1 hit
PIRSFiPIRSF000390 PLP_StrS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

P77690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGKAMSEF LPFSRPAMGV EELAAVKEVL ESGWITTGPK NQALEQAFCQ
60 70 80 90 100
LTGNQHAIAV SSATAGMHIT LMALKIGKGD EVITPSLTWV STLNMISLLG
110 120 130 140 150
ATPVMVDVDR DTLMVTPEAI ESAITPRTKA IIPVHYAGAP ADIDAIRAIG
160 170 180 190 200
ERYGIAVIED AAHAVGTYYK GRHIGAKGTA IFSFHAIKNI TCAEGGLIVT
210 220 230 240 250
DNENLARQLR MLKFHGLGVD AYDRQTWGRA PQAEVLTPGY KYNLTDINAA
260 270 280 290 300
IALTQLVKLE HLNTRRREIA QQYQQALAAL PFQPLSLPAW PHVHAWHLFI
310 320 330 340 350
IRVDEQRCGI SRDALMEALK ERGIGTGLHF RAAHTQKYYR ERFPTLSLPN
360 370 380
TEWNSERICS LPLFPDMTTA DADHVITALQ QLAGQ
Length:385
Mass (Da):42,238
Last modified:November 30, 2016 - v3
Checksum:i2017E3C950FFF4B8
GO

Sequence cautioni

The sequence AAC75313 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF527386 Genomic DNA Translation: AAM92146.1
U00096 Genomic DNA Translation: AAC75313.3 Different initiation.
AP009048 Genomic DNA Translation: BAA16076.2
PIRiC64996
RefSeqiNP_416756.5, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC75313; AAC75313; b2253
BAA16076; BAA16076; BAA16076
GeneIDi947375
KEGGiecj:JW5372
eco:b2253
PATRICifig|511145.12.peg.2345

Similar proteinsi

Entry informationi

Entry nameiARNB_ECOLI
AccessioniPrimary (citable) accession number: P77690
Secondary accession number(s): Q8KRQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 30, 2016
Last modified: February 28, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome