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P77671

- ALLB_ECOLI

UniProt

P77671 - ALLB_ECOLI

Protein

Allantoinase

Gene

allB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring.2 Publications

    Catalytic activityi

    (S)-allantoin + H2O = allantoate.1 Publication

    Cofactori

    Binds 2 zinc ions per subunit Probable. Can also use nickel, cobalt or manganese.2 PublicationsCurated

    Enzyme regulationi

    Severely inhibited by copper, and competitively inhibited by dithiothreitol (DTT). Zinc concentrations above 2.5 mM and cobalt or nickel concentrations above 1 mM are inhibitors.2 Publications

    Kineticsi

    1. KM=4.2 mM for allantoin (at 40 degrees Celsius and pH 8.0)2 Publications
    2. KM=17.0 mM for allantoin (in zinc-supplemented medium at 37 degrees Celsius and pH 7.4)2 Publications
    3. KM=19.5 mM for allantoin (in cobalt-supplemented medium at 37 degrees Celsius and pH 7.4)2 Publications
    4. KM=80.0 mM for allantoin (in nickel-supplemented medium at 37 degrees Celsius and pH 7.4)2 Publications

    Vmax=101 µmol/min/mg enzyme (in zinc-supplemented medium at 37 degrees Celsius and pH 7.4)2 Publications

    Vmax=8.1 µmol/min/mg enzyme (in cobalt-supplemented medium (20mM) at 37 degrees Celsius and pH 7.4)2 Publications

    Vmax=6.7 µmol/min/mg enzyme (at 40 degrees Celsius and pH 8.0)2 Publications

    Vmax=3.9 µmol/min/mg enzyme (in cobalt-supplemented medium (2mM) at 37 degrees Celsius and pH 7.4)2 Publications

    Vmax=0.7 µmol/min/mg enzyme (without added metal at 37 degrees Celsius and pH 7.4)2 Publications

    pH dependencei

    Optimum pH is 7.5-8.0. The optimal activity is at pH 8.8 for zinc-containing form and pH 9.0 for cobalt-containing form.2 Publications

    Temperature dependencei

    Optimum temperature is 40-45 degrees Celsius.2 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi59 – 591Zinc 1By similarity
    Metal bindingi61 – 611Zinc 1By similarity
    Metal bindingi146 – 1461Zinc 1; via carbamate groupBy similarity
    Metal bindingi146 – 1461Zinc 2; via carbamate groupBy similarity
    Metal bindingi186 – 1861Zinc 2By similarity
    Metal bindingi242 – 2421Zinc 2By similarity
    Metal bindingi315 – 3151Zinc 1By similarity

    GO - Molecular functioni

    1. allantoinase activity Source: EcoCyc
    2. cobalt ion binding Source: InterPro
    3. zinc ion binding Source: EcoCyc

    GO - Biological processi

    1. allantoin assimilation pathway Source: EcoCyc
    2. purine nucleobase metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Purine metabolism

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:G6281-MONOMER.
    ECOL316407:JW0500-MONOMER.
    MetaCyc:G6281-MONOMER.
    SABIO-RKP77671.
    UniPathwayiUPA00395; UER00653.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Allantoinase (EC:3.5.2.5)
    Alternative name(s):
    Allantoin-utilizing enzyme
    Gene namesi
    Name:allB
    Synonyms:glxB3, ybbX
    Ordered Locus Names:b0512, JW0500
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13619. allB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: EcoliWiki

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 453453AllantoinasePRO_0000165942Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei146 – 1461N6-carboxylysineBy similarity

    Post-translational modificationi

    Carbamylation allows a single lysine to coordinate two zinc ions.By similarity

    Expressioni

    Inductioni

    By glyoxylate.

    Gene expression databases

    GenevestigatoriP77671.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    IntActiP77671. 7 interactions.
    STRINGi511145.b0512.

    Structurei

    Secondary structure

    1
    453
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 97
    Beta strandi11 – 133
    Beta strandi18 – 203
    Beta strandi22 – 265
    Beta strandi29 – 357
    Beta strandi40 – 456
    Beta strandi50 – 534
    Beta strandi55 – 606
    Helixi73 – 8210
    Beta strandi85 – 906
    Beta strandi93 – 975
    Helixi102 – 11211
    Turni113 – 1153
    Beta strandi117 – 1226
    Turni131 – 1333
    Helixi134 – 1407
    Beta strandi145 – 1484
    Helixi166 – 17914
    Beta strandi183 – 1864
    Helixi190 – 20011
    Turni201 – 2044
    Helixi208 – 2136
    Helixi217 – 23418
    Beta strandi238 – 2403
    Helixi246 – 25712
    Beta strandi262 – 2665
    Helixi269 – 2724
    Helixi275 – 2817
    Helixi283 – 2853
    Helixi294 – 30512
    Turni321 – 3244
    Turni328 – 3303
    Helixi338 – 3403
    Helixi341 – 3499
    Turni350 – 3534
    Helixi357 – 3648
    Helixi366 – 3716
    Beta strandi388 – 3947
    Helixi401 – 4033
    Beta strandi406 – 4083
    Turni412 – 4154
    Beta strandi417 – 4193
    Beta strandi421 – 4277
    Beta strandi430 – 4345
    Turni435 – 4373

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3E74X-ray2.10A/B/C/D1-453[»]
    ProteinModelPortaliP77671.
    SMRiP77671. Positions 2-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP77671.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DHOase family. Allantoinase subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0044.
    HOGENOMiHOG000219146.
    KOiK01466.
    OMAiCSPWEGH.
    OrthoDBiEOG6KHFW6.
    PhylomeDBiP77671.

    Family and domain databases

    Gene3Di2.30.40.10. 1 hit.
    HAMAPiMF_01645. Hydantoinase.
    InterProiIPR017593. Allantoinase.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view]
    SUPFAMiSSF51338. SSF51338. 2 hits.
    TIGRFAMsiTIGR03178. allantoinase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P77671-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFDLIIKNG TVILENEARV VDIAVKGGKI AAIGQDLGDA KEVMDASGLV    50
    VSPGMVDAHT HISEPGRSHW EGYETGTRAA AKGGITTMIE MPLNQLPATV 100
    DRASIELKFD AAKGKLTIDA AQLGGLVSYN IDRLHELDEV GVVGFKCFVA 150
    TCGDRGIDND FRDVNDWQFF KGAQKLGELG QPVLVHCENA LICDELGEEA 200
    KREGRVTAHD YVASRPVFTE VEAIRRVLYL AKVAGCRLHV CHVSSPEGVE 250
    EVTRARQEGQ DVTCESCPHY FVLDTDQFEE IGTLAKCSPP IRDLENQKGM 300
    WEKLFNGEID CLVSDHSPCP PEMKAGNIMK AWGGIAGLQS CMDVMFDEAV 350
    QKRGMSLPMF GKLMATNAAD IFGLQQKGRI APGKDADFVF IQPNSSYVLT 400
    NDDLEYRHKV SPYVGRTIGA RITKTILRGD VIYDIEQGFP VAPKGQFILK 450
    HQQ 453
    Length:453
    Mass (Da):49,602
    Last modified:February 1, 1997 - v1
    Checksum:i5CFE588F94268AB4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89279 Genomic DNA. Translation: AAB93853.1.
    U82664 Genomic DNA. Translation: AAB40264.1.
    U00096 Genomic DNA. Translation: AAC73614.1.
    AP009048 Genomic DNA. Translation: BAE76290.1.
    PIRiG64782.
    RefSeqiNP_415045.1. NC_000913.3.
    YP_488802.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73614; AAC73614; b0512.
    BAE76290; BAE76290; BAE76290.
    GeneIDi12930868.
    945134.
    KEGGiecj:Y75_p0498.
    eco:b0512.
    PATRICi32116181. VBIEscCol129921_0532.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89279 Genomic DNA. Translation: AAB93853.1 .
    U82664 Genomic DNA. Translation: AAB40264.1 .
    U00096 Genomic DNA. Translation: AAC73614.1 .
    AP009048 Genomic DNA. Translation: BAE76290.1 .
    PIRi G64782.
    RefSeqi NP_415045.1. NC_000913.3.
    YP_488802.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3E74 X-ray 2.10 A/B/C/D 1-453 [» ]
    ProteinModelPortali P77671.
    SMRi P77671. Positions 2-451.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P77671. 7 interactions.
    STRINGi 511145.b0512.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73614 ; AAC73614 ; b0512 .
    BAE76290 ; BAE76290 ; BAE76290 .
    GeneIDi 12930868.
    945134.
    KEGGi ecj:Y75_p0498.
    eco:b0512.
    PATRICi 32116181. VBIEscCol129921_0532.

    Organism-specific databases

    EchoBASEi EB3384.
    EcoGenei EG13619. allB.

    Phylogenomic databases

    eggNOGi COG0044.
    HOGENOMi HOG000219146.
    KOi K01466.
    OMAi CSPWEGH.
    OrthoDBi EOG6KHFW6.
    PhylomeDBi P77671.

    Enzyme and pathway databases

    UniPathwayi UPA00395 ; UER00653 .
    BioCyci EcoCyc:G6281-MONOMER.
    ECOL316407:JW0500-MONOMER.
    MetaCyc:G6281-MONOMER.
    SABIO-RK P77671.

    Miscellaneous databases

    EvolutionaryTracei P77671.
    PROi P77671.

    Gene expression databases

    Genevestigatori P77671.

    Family and domain databases

    Gene3Di 2.30.40.10. 1 hit.
    HAMAPi MF_01645. Hydantoinase.
    InterProi IPR017593. Allantoinase.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view ]
    SUPFAMi SSF51338. SSF51338. 2 hits.
    TIGRFAMsi TIGR03178. allantoinase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli."
      Cusa E., Obradors N., Baldoma L., Badia J., Aguilar J.
      J. Bacteriol. 181:7479-7484(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: K12 / ECL1.
    2. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Functional expression and characterization of the two cyclic amidohydrolase enzymes, allantoinase and a novel phenylhydantoinase, from Escherichia coli."
      Kim G.J., Lee D.E., Kim H.-S.
      J. Bacteriol. 182:7021-7028(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION, FUNCTION, SUBUNIT.
      Strain: K12.
    6. "Metal ion dependence of recombinant Escherichia coli allantoinase."
      Mulrooney S.B., Hausinger R.P.
      J. Bacteriol. 185:126-134(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, STEREOSPECIFICITY, COFACTOR.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiALLB_ECOLI
    AccessioniPrimary (citable) accession number: P77671
    Secondary accession number(s): Q2MBR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The zinc-containing form utilizes only the (S)-isomer of allantoin, whereas the cobalt-containing form prefers the (S)-isomer, but also hydrolyzes the (R)-isomer at about 1/10 the rate.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3