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Protein

Hydrogenase-4 component I

Gene

hyfI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi47Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi111Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi141Iron-sulfur (4Fe-4S)Sequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-151.
ECOL316407:JW5805-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 component I (EC:1.-.-.-)
Gene namesi
Name:hyfI
Ordered Locus Names:b2489, JW5805
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14217. hyfI.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001187811 – 252Hydrogenase-4 component IAdd BLAST252

Proteomic databases

PaxDbiP77668.
PRIDEiP77668.

Interactioni

Protein-protein interaction databases

BioGridi4262153. 7 interactors.
STRINGi511145.b2489.

Structurei

3D structure databases

ProteinModelPortaliP77668.
SMRiP77668.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105C47. Bacteria.
COG3260. LUCA.
HOGENOMiHOG000228250.
InParanoidiP77668.
KOiK12144.
OMAiLRIYHAM.
PhylomeDBiP77668.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77668-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPVLTQHVS QPITLDEQTQ KMKRHLLQDI RRSAYVYRVD CGGCNACEIE
60 70 80 90 100
IFAAITPVFD AERFGIKVVS SPRHADILLF TGAVTRAMRM PALRAYESAP
110 120 130 140 150
DHKICVSYGA CGVGGGIFHD LYSVWGGSDT IVPIDVWIPG CPPTPAATIH
160 170 180 190 200
GFAVALGLLQ QKIHAVDYRD PTGVTMQPLW PQIPPSQRIA IEREARRLAG
210 220 230 240 250
YRQGREICDR LLRHLSDDPT GNRVNTWLRD ADDPRLNSIV QQLFRVLRGL

HD
Length:252
Mass (Da):28,101
Last modified:February 1, 1997 - v1
Checksum:i3BA49D62ABAEAEEE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti250 – 252LHD → VT (PubMed:9205837).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88571.1.
U00096 Genomic DNA. Translation: AAC75542.1.
AP009048 Genomic DNA. Translation: BAA16377.2.
PIRiH65024.
RefSeqiNP_416984.1. NC_000913.3.
WP_000075558.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75542; AAC75542; b2489.
BAA16377; BAA16377; BAA16377.
GeneIDi946966.
KEGGiecj:JW5805.
eco:b2489.
PATRICi32120365. VBIEscCol129921_2585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88571.1.
U00096 Genomic DNA. Translation: AAC75542.1.
AP009048 Genomic DNA. Translation: BAA16377.2.
PIRiH65024.
RefSeqiNP_416984.1. NC_000913.3.
WP_000075558.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77668.
SMRiP77668.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262153. 7 interactors.
STRINGi511145.b2489.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP77668.
PRIDEiP77668.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75542; AAC75542; b2489.
BAA16377; BAA16377; BAA16377.
GeneIDi946966.
KEGGiecj:JW5805.
eco:b2489.
PATRICi32120365. VBIEscCol129921_2585.

Organism-specific databases

EchoBASEiEB3969.
EcoGeneiEG14217. hyfI.

Phylogenomic databases

eggNOGiENOG4105C47. Bacteria.
COG3260. LUCA.
HOGENOMiHOG000228250.
InParanoidiP77668.
KOiK12144.
OMAiLRIYHAM.
PhylomeDBiP77668.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-151.
ECOL316407:JW5805-MONOMER.

Miscellaneous databases

PROiP77668.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYFI_ECOLI
AccessioniPrimary (citable) accession number: P77668
Secondary accession number(s): P76975
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.