Reviewed, UniProtKB/Swiss-Prot P77668 (HYFI_ECOLI)

Names and originHide

Protein names

Recommended name:
Hydrogenase-4 component I
EC=1.-.-.-

Gene names

Name:	hyfI
Ordered Locus Names:	b2489, JW5805

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributesHide

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Cofactor	Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

OntologiesHide

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Hydrogenase-4 component I		PRO_0000118781
Sites
	Metal binding	41	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	47	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	111	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	141	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	250 – 252	3	LHD → VT Ref.2

SequencesHide

	Sequence		Length	Mass (Da)
	P77668-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 3BA49D62ABAEAEEE	FASTA	252	28,101
	10 20 30 40 50 60 MSPVLTQHVS QPITLDEQTQ KMKRHLLQDI RRSAYVYRVD CGGCNACEIE IFAAITPVFD 70 80 90 100 110 120 AERFGIKVVS SPRHADILLF TGAVTRAMRM PALRAYESAP DHKICVSYGA CGVGGGIFHD 130 140 150 160 170 180 LYSVWGGSDT IVPIDVWIPG CPPTPAATIH GFAVALGLLQ QKIHAVDYRD PTGVTMQPLW 190 200 210 220 230 240 PQIPPSQRIA IEREARRLAG YRQGREICDR LLRHLSDDPT GNRVNTWLRD ADDPRLNSIV 250 QQLFRVLRGL HD

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 250-252. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	M63654 Genomic DNA. Translation: AAB88571.1. U00096 Genomic DNA. Translation: AAC75542.1. AP009048 Genomic DNA. Translation: BAA16377.2.
PIR	H65024.
RefSeq	AP_003075.1. NP_416984.1.
3D structure databases
SMR	P77668. Positions 31-169.
ModBase	Search...
Protein-protein interaction databases
STRING	P77668.
Genome annotation databases
EnsemblBacteria	EBESCT00000001663; EBESCP00000001663; EBESCG00000001374. EBESCT00000016967; EBESCP00000016258; EBESCG00000016026.
GeneID	946966.
GenomeReviews	Gene locus JW5805 in contig AP009048_GR. Gene locus b2489 in contig U00096_GR.
KEGG	ecj:JW5805. eco:b2489.
Organism-specific databases
EchoBASE	EB3969.
EcoGene	EG14217. hyfI.
CMR	Search...
Phylogenomic databases
eggNOG	COG3260.
HOGENOM	HBG553221.
OMA	THIEGED.
ProtClustDB	CLSK880408.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-151. ECOL168927:B2489-MONOMER.
Gene expression databases
Genevestigator	P77668.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UbQ_OxRdtase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. [Graphical view]
PANTHER	PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

HYFI_ECOLI

Accession

Primary (citable) accession number: P77668
Secondary accession number(s): P76975

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	July 13, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)