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P77668 (HYFI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hydrogenase-4 component I

EC=1.-.-.-
Gene names
Name:hyfI
Ordered Locus Names:b2489, JW5805
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Cofactor

Binds 1 4Fe-4S cluster Potential.

Sequence similarities

Belongs to the complex I 20 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 252252Hydrogenase-4 component I
PRO_0000118781

Sites

Metal binding411Iron-sulfur (4Fe-4S) Potential
Metal binding471Iron-sulfur (4Fe-4S) Potential
Metal binding1111Iron-sulfur (4Fe-4S) Potential
Metal binding1411Iron-sulfur (4Fe-4S) Potential

Experimental info

Sequence conflict250 – 2523LHD → VT Ref.2

Sequences

Sequence LengthMass (Da)Tools
P77668 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 3BA49D62ABAEAEEE

FASTA25228,101
        10         20         30         40         50         60 
MSPVLTQHVS QPITLDEQTQ KMKRHLLQDI RRSAYVYRVD CGGCNACEIE IFAAITPVFD 

        70         80         90        100        110        120 
AERFGIKVVS SPRHADILLF TGAVTRAMRM PALRAYESAP DHKICVSYGA CGVGGGIFHD 

       130        140        150        160        170        180 
LYSVWGGSDT IVPIDVWIPG CPPTPAATIH GFAVALGLLQ QKIHAVDYRD PTGVTMQPLW 

       190        200        210        220        230        240 
PQIPPSQRIA IEREARRLAG YRQGREICDR LLRHLSDDPT GNRVNTWLRD ADDPRLNSIV 

       250 
QQLFRVLRGL HD 

« Hide

References

« Hide 'large scale' references
[1]Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 250-252.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63654 Genomic DNA. Translation: AAB88571.1.
U00096 Genomic DNA. Translation: AAC75542.1.
AP009048 Genomic DNA. Translation: BAA16377.2.
PIRH65024.
RefSeqNP_416984.1. NC_000913.2.

3D structure databases

ProteinModelPortalP77668.
SMRP77668. Positions 31-174.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000001663; EBESCP00000001663; EBESCG00000001374.
EBESCT00000016967; EBESCP00000016258; EBESCG00000016026.
GeneID946966.
GenomeReviewsGene locus JW5805 in contig AP009048_GR.
Gene locus b2489 in contig U00096_GR.
KEGGecj:JW5805.
eco:b2489.
PATRIC32120365. VBIEscCol129921_2585.

Organism-specific databases

EchoBASEEB3969.
EcoGeneEG14217. hyfI.

Phylogenomic databases

eggNOGCOG3260.
GeneTreeEBGT00050000009829.
HOGENOMHBG553221.
OMATHIEGED.
PhylomeDBP77668.
ProtClustDBCLSK880408.

Enzyme and pathway databases

BioCycEcoCyc:MONOMER0-151.

Gene expression databases

GenevestigatorP77668.

Family and domain databases

InterProIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
IPR014406. NiFe-hyd_3_ssu/Q_oxred_NuoB.
[Graphical view]
Gene3DG3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
KOK12144.
PANTHERPTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
PfamPF01058. Oxidored_q6. 1 hit.
[Graphical view]
PROSITEPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHYFI_ECOLI
AccessionPrimary (citable) accession number: P77668
Secondary accession number(s): P76975
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families