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P77650

- HCAD_ECOLI

UniProt

P77650 - HCAD_ECOLI

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Protein
3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component
Gene
hcaD, hcaA4, phdA, yfhY, b2542, JW2526
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.UniRule annotation

Catalytic activityi

Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.UniRule annotation

Cofactori

FAD.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi5 – 3632FAD Reviewed prediction
Add
BLAST
Nucleotide bindingi146 – 17429NAD Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. 3-phenylpropionate dioxygenase activity Source: UniProtKB-HAMAP
  2. ferredoxin-NAD+ reductase activity Source: UniProtKB-EC
  3. flavin adenine dinucleotide binding Source: InterPro
  4. protein binding Source: IntAct

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: EcoCyc
  2. cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:HCAD-MONOMER.
ECOL316407:JW2526-MONOMER.
MetaCyc:HCAD-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component (EC:1.18.1.3)
Alternative name(s):
Digoxigenin system ferredoxin--NAD(+) reductase component
Gene namesi
Name:hcaD
Synonyms:hcaA4, phdA, yfhY
Ordered Locus Names:b2542, JW2526
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13460. hcaD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4004003-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase componentUniRule annotation
PRO_0000167661Add
BLAST

Proteomic databases

PaxDbiP77650.
PRIDEiP77650.

PTM databases

PhosSiteiP0809399.

Expressioni

Gene expression databases

GenevestigatoriP77650.

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).

Binary interactionsi

WithEntry#Exp.IntActNotes
nikRP0A6Z62EBI-1129389,EBI-562488

Protein-protein interaction databases

IntActiP77650. 7 interactions.
STRINGi511145.b2542.

Structurei

3D structure databases

ProteinModelPortaliP77650.
SMRiP77650. Positions 5-386.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0446.
HOGENOMiHOG000276711.
KOiK00529.
OMAiRLPPPWF.
OrthoDBiEOG6T4RXM.
PhylomeDBiP77650.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
HAMAPiMF_01651. HcaD.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023744. HcaD.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR028202. Reductase_C.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF14759. Reductase_C. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

P77650-1 [UniParc]FASTAAdd to Basket

« Hide

MKEKTIIIVG GGQAAAMAAA SLRQQGFTGE LHLFSDERHL PYERPPLSKS    50
MLLEDSPQLQ QVLPANWWQE NNVHLHSGVT IKTLGRDTRE LVLTNGESWH 100
WDQLFIATGA AARPLPLLDA LGERCFTLRH AGDAARLREV LQPERSVVII 150
GAGTIGLELA ASATQRRCKV TVIELAATVM GRNAPPPVQR YLLQRHQQAG 200
VRILLNNAIE HVVDGEKVEL TLQSGETLQA DVVIYGIGIS ANEQLAREAN 250
LDTANGIVID EACRTCDPAI FAGGDVAITR LDNGALHRCE SWENANNQAQ 300
IAAAAMLGLP LPLLPPPWFW SDQYSDNLQF IGDMRGDDWL CRGNPETQKA 350
IWFNLQNGVL IGAVTLNQGR EIRPIRKWIQ SGKTFDAKLL IDENIALKSL 400
Length:400
Mass (Da):43,978
Last modified:February 1, 1997 - v1
Checksum:iF5A1A06C4F1DFF36
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11070 Genomic DNA. Translation: CAA71952.1.
U00096 Genomic DNA. Translation: AAC75595.1.
AP009048 Genomic DNA. Translation: BAA16445.1.
PIRiE65031.
RefSeqiNP_417037.1. NC_000913.3.
YP_490770.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75595; AAC75595; b2542.
BAA16445; BAA16445; BAA16445.
GeneIDi12930468.
945427.
KEGGiecj:Y75_p2495.
eco:b2542.
PATRICi32120481. VBIEscCol129921_2643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11070 Genomic DNA. Translation: CAA71952.1 .
U00096 Genomic DNA. Translation: AAC75595.1 .
AP009048 Genomic DNA. Translation: BAA16445.1 .
PIRi E65031.
RefSeqi NP_417037.1. NC_000913.3.
YP_490770.1. NC_007779.1.

3D structure databases

ProteinModelPortali P77650.
SMRi P77650. Positions 5-386.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P77650. 7 interactions.
STRINGi 511145.b2542.

PTM databases

PhosSitei P0809399.

Proteomic databases

PaxDbi P77650.
PRIDEi P77650.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75595 ; AAC75595 ; b2542 .
BAA16445 ; BAA16445 ; BAA16445 .
GeneIDi 12930468.
945427.
KEGGi ecj:Y75_p2495.
eco:b2542.
PATRICi 32120481. VBIEscCol129921_2643.

Organism-specific databases

EchoBASEi EB3233.
EcoGenei EG13460. hcaD.

Phylogenomic databases

eggNOGi COG0446.
HOGENOMi HOG000276711.
KOi K00529.
OMAi RLPPPWF.
OrthoDBi EOG6T4RXM.
PhylomeDBi P77650.

Enzyme and pathway databases

UniPathwayi UPA00714 .
BioCyci EcoCyc:HCAD-MONOMER.
ECOL316407:JW2526-MONOMER.
MetaCyc:HCAD-MONOMER.

Miscellaneous databases

PROi P77650.

Gene expression databases

Genevestigatori P77650.

Family and domain databases

Gene3Di 3.30.390.30. 1 hit.
HAMAPi MF_01651. HcaD.
InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023744. HcaD.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR028202. Reductase_C.
[Graphical view ]
Pfami PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF14759. Reductase_C. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF55424. SSF55424. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12."
    Diaz E., Ferrandez A., Garcia J.L.
    J. Bacteriol. 180:2915-2923(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiHCAD_ECOLI
AccessioniPrimary (citable) accession number: P77650
Secondary accession number(s): O08100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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