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Protein

3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component

Gene

hcaD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.

Catalytic activityi

Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.

Cofactori

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi5 – 36FADSequence analysisAdd BLAST32
Nucleotide bindingi146 – 174NADSequence analysisAdd BLAST29

GO - Molecular functioni

GO - Biological processi

  • 3-phenylpropionate catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processAromatic hydrocarbons catabolism
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:HCAD-MONOMER
MetaCyc:HCAD-MONOMER
UniPathwayiUPA00714

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component (EC:1.18.1.3)
Gene namesi
Name:hcaD
Synonyms:hcaA4, phdA, yfhY
Ordered Locus Names:b2542, JW2526
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13460 hcaD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001676611 – 4003-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase componentAdd BLAST400

Proteomic databases

PaxDbiP77650
PRIDEiP77650

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).

Binary interactionsi

WithEntry#Exp.IntActNotes
nikRP0A6Z62EBI-1129389,EBI-562488

Protein-protein interaction databases

BioGridi425946212 interactors.
IntActiP77650 7 interactors.
STRINGi316385.ECDH10B_2709

Structurei

3D structure databases

ProteinModelPortaliP77650
SMRiP77650
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108EQM Bacteria
COG0446 LUCA
HOGENOMiHOG000276711
InParanoidiP77650
KOiK00529
OMAiGAQLRYC
PhylomeDBiP77650

Family and domain databases

Gene3Di3.30.390.301 hit
3.50.50.603 hits
HAMAPiMF_01651 HcaD, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR023744 HcaD
IPR028202 Reductase_C
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF14759 Reductase_C, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit

Sequencei

Sequence statusi: Complete.

P77650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKTIIIVG GGQAAAMAAA SLRQQGFTGE LHLFSDERHL PYERPPLSKS
60 70 80 90 100
MLLEDSPQLQ QVLPANWWQE NNVHLHSGVT IKTLGRDTRE LVLTNGESWH
110 120 130 140 150
WDQLFIATGA AARPLPLLDA LGERCFTLRH AGDAARLREV LQPERSVVII
160 170 180 190 200
GAGTIGLELA ASATQRRCKV TVIELAATVM GRNAPPPVQR YLLQRHQQAG
210 220 230 240 250
VRILLNNAIE HVVDGEKVEL TLQSGETLQA DVVIYGIGIS ANEQLAREAN
260 270 280 290 300
LDTANGIVID EACRTCDPAI FAGGDVAITR LDNGALHRCE SWENANNQAQ
310 320 330 340 350
IAAAAMLGLP LPLLPPPWFW SDQYSDNLQF IGDMRGDDWL CRGNPETQKA
360 370 380 390 400
IWFNLQNGVL IGAVTLNQGR EIRPIRKWIQ SGKTFDAKLL IDENIALKSL
Length:400
Mass (Da):43,978
Last modified:February 1, 1997 - v1
Checksum:iF5A1A06C4F1DFF36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11070 Genomic DNA Translation: CAA71952.1
U00096 Genomic DNA Translation: AAC75595.1
AP009048 Genomic DNA Translation: BAA16445.1
PIRiE65031
RefSeqiNP_417037.1, NC_000913.3
WP_000660788.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75595; AAC75595; b2542
BAA16445; BAA16445; BAA16445
GeneIDi945427
KEGGiecj:JW2526
eco:b2542
PATRICifig|1411691.4.peg.4192

Similar proteinsi

Entry informationi

Entry nameiHCAD_ECOLI
AccessioniPrimary (citable) accession number: P77650
Secondary accession number(s): O08100
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome