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Protein

Sugar phosphatase YfbT

Gene

yfbT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of different sugar phosphates including ribose-5-phosphate (Ribu5P), fructose-1-phosphate (Fru1P), fructose-6-phosphate (Fru6P), glucose-6-phosphate (Glu6P). Selectively hydrolyzes beta-D-glucose-1-phosphate (bGlu1P) and has no activity with the alpha form.2 Publications

Catalytic activityi

Sugar phosphate + H2O = sugar + phosphate.

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Magnesium. Can also use other divalent metal cations as manganese, cobalt or zinc.1 Publication

Kineticsi

  1. KM=1 mM for Fru1P (with magnesium ions as cofactor and at pH 9)1 Publication
  2. KM=1.3 mM for Fru6P (with zinc ions as cofactor and at pH 9)1 Publication
  3. KM=1.6 mM for Glu6P (with cobalt ions as cofactor and at pH 9)1 Publication
  4. KM=2.2 mM for Ribu5P (with zinc ions as cofactor and at pH 9)1 Publication
  5. KM=7 mM for bGlu1P (with zinc ions as cofactor and at pH 9)1 Publication

    pH dependencei

    Optimum pH is between 6 and 7.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei9NucleophileBy similarity1
    Metal bindingi9MagnesiumBy similarity1
    Metal bindingi11MagnesiumBy similarity1
    Metal bindingi163MagnesiumBy similarity1

    GO - Molecular functioni

    • cobalt ion binding Source: UniProtKB
    • glycerol-3-phosphatase activity Source: EcoCyc
    • magnesium ion binding Source: UniProtKB
    • manganese ion binding Source: UniProtKB
    • sugar-phosphatase activity Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:G7187-MONOMER.
    ECOL316407:JW5376-MONOMER.
    MetaCyc:G7187-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sugar phosphatase YfbT (EC:3.1.3.23)
    Gene namesi
    Name:yfbT
    Ordered Locus Names:b2293, JW5376
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG14104. yfbT.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001080591 – 216Sugar phosphatase YfbTAdd BLAST216

    Proteomic databases

    EPDiP77625.
    PaxDbiP77625.
    PRIDEiP77625.

    Interactioni

    Protein-protein interaction databases

    BioGridi4262967. 8 interactors.
    DIPiDIP-11972N.
    IntActiP77625. 2 interactors.
    MINTiMINT-1275778.
    STRINGi511145.b2293.

    Structurei

    3D structure databases

    ProteinModelPortaliP77625.
    SMRiP77625.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni9 – 11Substrate bindingBy similarity3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105EVE. Bacteria.
    COG0637. LUCA.
    HOGENOMiHOG000248341.
    InParanoidiP77625.
    KOiK19270.
    OMAiPKENARH.
    PhylomeDBiP77625.

    Family and domain databases

    Gene3Di1.10.150.240. 1 hit.
    3.40.50.1000. 1 hit.
    InterProiIPR023214. HAD-like_dom.
    IPR006439. HAD-SF_hydro_IA.
    IPR023198. PGP_dom2.
    [Graphical view]
    PfamiPF13419. HAD_2. 1 hit.
    [Graphical view]
    PRINTSiPR00413. HADHALOGNASE.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01549. HAD-SF-IA-v1. 1 hit.
    TIGR01509. HAD-SF-IA-v3. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P77625-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRCKGFLFDL DGTLVDSLPA VERAWSNWAR RHGLAPEEVL AFIHGKQAIT
    60 70 80 90 100
    SLRHFMAGKS EADIAAEFTR LEHIEATETE GITALPGAIA LLSHLNKAGI
    110 120 130 140 150
    PWAIVTSGSM PVARARHKIA GLPAPEVFVT AERVKRGKPE PDAYLLGAQL
    160 170 180 190 200
    LGLAPQECVV VEDAPAGVLS GLAAGCHVIA VNAPADTPRL NEVDLVLHSL
    210
    EQITVTKQPN GDVIIQ
    Length:216
    Mass (Da):23,008
    Last modified:September 26, 2001 - v2
    Checksum:iC687DF877E2706EF
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75353.2.
    AP009048 Genomic DNA. Translation: BAA16129.2.
    PIRiC65001.
    RefSeqiNP_416796.2. NC_000913.3.
    WP_001203392.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75353; AAC75353; b2293.
    BAA16129; BAA16129; BAA16129.
    GeneIDi946777.
    KEGGiecj:JW5376.
    eco:b2293.
    PATRICi32119955. VBIEscCol129921_2387.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75353.2.
    AP009048 Genomic DNA. Translation: BAA16129.2.
    PIRiC65001.
    RefSeqiNP_416796.2. NC_000913.3.
    WP_001203392.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP77625.
    SMRiP77625.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262967. 8 interactors.
    DIPiDIP-11972N.
    IntActiP77625. 2 interactors.
    MINTiMINT-1275778.
    STRINGi511145.b2293.

    Proteomic databases

    EPDiP77625.
    PaxDbiP77625.
    PRIDEiP77625.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75353; AAC75353; b2293.
    BAA16129; BAA16129; BAA16129.
    GeneIDi946777.
    KEGGiecj:JW5376.
    eco:b2293.
    PATRICi32119955. VBIEscCol129921_2387.

    Organism-specific databases

    EchoBASEiEB3857.
    EcoGeneiEG14104. yfbT.

    Phylogenomic databases

    eggNOGiENOG4105EVE. Bacteria.
    COG0637. LUCA.
    HOGENOMiHOG000248341.
    InParanoidiP77625.
    KOiK19270.
    OMAiPKENARH.
    PhylomeDBiP77625.

    Enzyme and pathway databases

    BioCyciEcoCyc:G7187-MONOMER.
    ECOL316407:JW5376-MONOMER.
    MetaCyc:G7187-MONOMER.

    Miscellaneous databases

    PROiP77625.

    Family and domain databases

    Gene3Di1.10.150.240. 1 hit.
    3.40.50.1000. 1 hit.
    InterProiIPR023214. HAD-like_dom.
    IPR006439. HAD-SF_hydro_IA.
    IPR023198. PGP_dom2.
    [Graphical view]
    PfamiPF13419. HAD_2. 1 hit.
    [Graphical view]
    PRINTSiPR00413. HADHALOGNASE.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01549. HAD-SF-IA-v1. 1 hit.
    TIGR01509. HAD-SF-IA-v3. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiYFBT_ECOLI
    AccessioniPrimary (citable) accession number: P77625
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: September 26, 2001
    Last modified: November 2, 2016
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.