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Protein

2-keto-4-pentenoate hydratase

Gene

mhpD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.1 Publication

Catalytic activityi

(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-dienoate + H2O.

Cofactori

Mn2+1 PublicationNote: Divalent metal ions. Optimum activity is obtained with Mn2+.1 Publication

Enzyme regulationi

Inhibited by sodium oxalate.1 Publication

Kineticsi

  1. KM=41 µM for 2-hydroxy-pentadienoic acid (at pH 6)1 Publication

    pH dependencei

    Optimum pH is 5.5-8.0, but decreases linearly above pH 8.3, and shows an inflection at pH 5.5.1 Publication

    Pathwayi: 3-phenylpropanoate degradation

    This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
    View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

    GO - Molecular functioni

    • 2-oxopent-4-enoate hydratase activity Source: EcoCyc
    • identical protein binding Source: EcoCyc
    • manganese ion binding Source: EcoCyc

    GO - Biological processi

    • 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
    • aromatic compound catabolic process Source: EcoliWiki

    Keywordsi

    Molecular functionLyase
    Biological processAromatic hydrocarbons catabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:MHPDHYDROL-MONOMER
    MetaCyc:MHPDHYDROL-MONOMER
    BRENDAi4.2.1.80 2165
    SABIO-RKiP77608
    UniPathwayiUPA00714

    Chemistry databases

    SwissLipidsiSLP:000001886

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-keto-4-pentenoate hydratase (EC:4.2.1.80)
    Alternative name(s):
    2-hydroxypentadienoic acid hydratase
    Gene namesi
    Name:mhpD
    Ordered Locus Names:b0350, JW0341
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG14274 mhpD

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: EcoliWiki

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000964691 – 2692-keto-4-pentenoate hydrataseAdd BLAST269

    Proteomic databases

    PaxDbiP77608
    PRIDEiP77608

    Interactioni

    GO - Molecular functioni

    • identical protein binding Source: EcoCyc

    Protein-protein interaction databases

    BioGridi4260731, 5 interactors
    IntActiP77608, 3 interactors
    STRINGi316407.85674492

    Structurei

    Secondary structure

    1269
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 19Combined sources17
    Helixi27 – 30Combined sources4
    Helixi35 – 51Combined sources17
    Beta strandi56 – 62Combined sources7
    Helixi66 – 70Combined sources5
    Turni71 – 73Combined sources3
    Beta strandi78 – 83Combined sources6
    Helixi84 – 86Combined sources3
    Beta strandi87 – 89Combined sources3
    Beta strandi92 – 94Combined sources3
    Helixi96 – 98Combined sources3
    Beta strandi99 – 101Combined sources3
    Beta strandi103 – 114Combined sources12
    Helixi123 – 127Combined sources5
    Beta strandi130 – 140Combined sources11
    Helixi145 – 147Combined sources3
    Helixi151 – 156Combined sources6
    Helixi158 – 160Combined sources3
    Beta strandi163 – 166Combined sources4
    Beta strandi182 – 187Combined sources6
    Beta strandi190 – 196Combined sources7
    Helixi197 – 199Combined sources3
    Turni200 – 202Combined sources3
    Helixi204 – 217Combined sources14
    Beta strandi227 – 234Combined sources8
    Beta strandi244 – 249Combined sources6
    Turni250 – 252Combined sources3
    Beta strandi253 – 259Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1SV6X-ray2.90A/B/C/D/E1-269[»]
    2WQTX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-269[»]
    ProteinModelPortaliP77608
    SMRiP77608
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP77608

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105IKQ Bacteria
    COG3971 LUCA
    HOGENOMiHOG000179637
    InParanoidiP77608
    KOiK02554
    OMAiIRDWSIG
    PhylomeDBiP77608

    Family and domain databases

    Gene3Di3.90.850.10, 1 hit
    HAMAPiMF_01655 MhpD, 1 hit
    InterProiView protein in InterPro
    IPR011234 Fumarylacetoacetase-like_C
    IPR036663 Fumarylacetoacetase_C_sf
    IPR023793 Keto_pentenoate-hydratase
    PfamiView protein in Pfam
    PF01557 FAA_hydrolase, 1 hit
    SUPFAMiSSF56529 SSF56529, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P77608-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTKHTLEQLA ADLRRAAEQG EAIAPLRDLI GIDNAEAAYA IQHINVQHDV
    60 70 80 90 100
    AQGRRVVGRK VGLTHPKVQQ QLGVDQPDFG TLFADMCYGD NEIIPFSRVL
    110 120 130 140 150
    QPRIEAEIAL VLNRDLPATD ITFDELYNAI EWVLPALEVV GSRIRDWSIQ
    160 170 180 190 200
    FVDTVADNAS CGVYVIGGPA QRPAGLDLKN CAMKMTRNNE EVSSGRGSEC
    210 220 230 240 250
    LGHPLNAAVW LARKMASLGE PLRTGDIILT GALGPMVAVN AGDRFEAHIE
    260
    GIGSVAATFS SAAPKGSLS
    Length:269
    Mass (Da):28,890
    Last modified:July 15, 1998 - v2
    Checksum:i34A81A8A4E236358
    GO

    Sequence cautioni

    The sequence AAB18074 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAA13055 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti202G → E in BAA13055 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D86239 Genomic DNA Translation: BAA13055.1 Different initiation.
    Y09555 Genomic DNA Translation: CAA70750.1
    U73857 Genomic DNA Translation: AAB18074.1 Different initiation.
    U00096 Genomic DNA Translation: AAC73453.2
    AP009048 Genomic DNA Translation: BAE76132.1
    PIRiF64762
    RefSeqiNP_414884.2, NC_000913.3
    WP_000160710.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73453; AAC73453; b0350
    BAE76132; BAE76132; BAE76132
    GeneIDi944768
    KEGGiecj:JW0341
    eco:b0350
    PATRICifig|1411691.4.peg.1928

    Similar proteinsi

    Entry informationi

    Entry nameiMHPD_ECOLI
    AccessioniPrimary (citable) accession number: P77608
    Secondary accession number(s): P71205, P77045, Q2MC74
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 15, 1998
    Last modified: April 25, 2018
    This is version 129 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health