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Protein

2-keto-4-pentenoate hydratase

Gene

mhpD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.1 Publication

Catalytic activityi

4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O.

Cofactori

Mn2+1 PublicationNote: Divalent metal ions. Optimum activity is obtained with Mn2+.1 Publication

Enzyme regulationi

Inhibited by sodium oxalate.1 Publication

Kineticsi

  1. KM=41 µM for 2-hydroxy-pentadienoic acid (at pH 6)1 Publication

    pH dependencei

    Optimum pH is 5.5-8.0, but decreases linearly above pH 8.3, and shows an inflection at pH 5.5.1 Publication

    Pathwayi: 3-phenylpropanoate degradation

    This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
    View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

    GO - Molecular functioni

    • 2-oxopent-4-enoate hydratase activity Source: EcoCyc
    • identical protein binding Source: EcoCyc
    • manganese ion binding Source: EcoCyc

    GO - Biological processi

    • 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
    • aromatic compound catabolic process Source: EcoliWiki
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:MHPDHYDROL-MONOMER.
    ECOL316407:JW0341-MONOMER.
    MetaCyc:MHPDHYDROL-MONOMER.
    BRENDAi4.2.1.80. 2165.
    SABIO-RKP77608.
    UniPathwayiUPA00714.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-keto-4-pentenoate hydratase (EC:4.2.1.80)
    Alternative name(s):
    2-hydroxypentadienoic acid hydratase
    Gene namesi
    Name:mhpD
    Ordered Locus Names:b0350, JW0341
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG14274. mhpD.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: EcoliWiki
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000964691 – 2692-keto-4-pentenoate hydrataseAdd BLAST269

    Proteomic databases

    PaxDbiP77608.
    PRIDEiP77608.

    Interactioni

    GO - Molecular functioni

    • identical protein binding Source: EcoCyc

    Protein-protein interaction databases

    BioGridi4260731. 5 interactors.
    IntActiP77608. 3 interactors.
    STRINGi511145.b0350.

    Structurei

    Secondary structure

    1269
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 19Combined sources17
    Helixi27 – 30Combined sources4
    Helixi35 – 51Combined sources17
    Beta strandi56 – 62Combined sources7
    Helixi66 – 70Combined sources5
    Turni71 – 73Combined sources3
    Beta strandi78 – 83Combined sources6
    Helixi84 – 86Combined sources3
    Beta strandi87 – 89Combined sources3
    Beta strandi92 – 94Combined sources3
    Helixi96 – 98Combined sources3
    Beta strandi99 – 101Combined sources3
    Beta strandi103 – 114Combined sources12
    Helixi123 – 127Combined sources5
    Beta strandi130 – 140Combined sources11
    Helixi145 – 147Combined sources3
    Helixi151 – 156Combined sources6
    Helixi158 – 160Combined sources3
    Beta strandi163 – 166Combined sources4
    Beta strandi182 – 187Combined sources6
    Beta strandi190 – 196Combined sources7
    Helixi197 – 199Combined sources3
    Turni200 – 202Combined sources3
    Helixi204 – 217Combined sources14
    Beta strandi227 – 234Combined sources8
    Beta strandi244 – 249Combined sources6
    Turni250 – 252Combined sources3
    Beta strandi253 – 259Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1SV6X-ray2.90A/B/C/D/E1-269[»]
    2WQTX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-269[»]
    ProteinModelPortaliP77608.
    SMRiP77608.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP77608.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105IKQ. Bacteria.
    COG3971. LUCA.
    HOGENOMiHOG000179637.
    InParanoidiP77608.
    KOiK02554.
    OMAiIRDWSIG.
    PhylomeDBiP77608.

    Family and domain databases

    Gene3Di3.90.850.10. 1 hit.
    HAMAPiMF_01655. MhpD. 1 hit.
    InterProiIPR011234. Fumarylacetoacetase_C-rel.
    IPR023793. Keto_pentenoate-hydratase.
    [Graphical view]
    PfamiPF01557. FAA_hydrolase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56529. SSF56529. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P77608-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTKHTLEQLA ADLRRAAEQG EAIAPLRDLI GIDNAEAAYA IQHINVQHDV
    60 70 80 90 100
    AQGRRVVGRK VGLTHPKVQQ QLGVDQPDFG TLFADMCYGD NEIIPFSRVL
    110 120 130 140 150
    QPRIEAEIAL VLNRDLPATD ITFDELYNAI EWVLPALEVV GSRIRDWSIQ
    160 170 180 190 200
    FVDTVADNAS CGVYVIGGPA QRPAGLDLKN CAMKMTRNNE EVSSGRGSEC
    210 220 230 240 250
    LGHPLNAAVW LARKMASLGE PLRTGDIILT GALGPMVAVN AGDRFEAHIE
    260
    GIGSVAATFS SAAPKGSLS
    Length:269
    Mass (Da):28,890
    Last modified:July 15, 1998 - v2
    Checksum:i34A81A8A4E236358
    GO

    Sequence cautioni

    The sequence AAB18074 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAA13055 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti202G → E in BAA13055 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D86239 Genomic DNA. Translation: BAA13055.1. Different initiation.
    Y09555 Genomic DNA. Translation: CAA70750.1.
    U73857 Genomic DNA. Translation: AAB18074.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73453.2.
    AP009048 Genomic DNA. Translation: BAE76132.1.
    PIRiF64762.
    RefSeqiNP_414884.2. NC_000913.3.
    WP_000160710.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73453; AAC73453; b0350.
    BAE76132; BAE76132; BAE76132.
    GeneIDi944768.
    KEGGiecj:JW0341.
    eco:b0350.
    PATRICi32115833. VBIEscCol129921_0358.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D86239 Genomic DNA. Translation: BAA13055.1. Different initiation.
    Y09555 Genomic DNA. Translation: CAA70750.1.
    U73857 Genomic DNA. Translation: AAB18074.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73453.2.
    AP009048 Genomic DNA. Translation: BAE76132.1.
    PIRiF64762.
    RefSeqiNP_414884.2. NC_000913.3.
    WP_000160710.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1SV6X-ray2.90A/B/C/D/E1-269[»]
    2WQTX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-269[»]
    ProteinModelPortaliP77608.
    SMRiP77608.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260731. 5 interactors.
    IntActiP77608. 3 interactors.
    STRINGi511145.b0350.

    Proteomic databases

    PaxDbiP77608.
    PRIDEiP77608.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73453; AAC73453; b0350.
    BAE76132; BAE76132; BAE76132.
    GeneIDi944768.
    KEGGiecj:JW0341.
    eco:b0350.
    PATRICi32115833. VBIEscCol129921_0358.

    Organism-specific databases

    EchoBASEiEB4022.
    EcoGeneiEG14274. mhpD.

    Phylogenomic databases

    eggNOGiENOG4105IKQ. Bacteria.
    COG3971. LUCA.
    HOGENOMiHOG000179637.
    InParanoidiP77608.
    KOiK02554.
    OMAiIRDWSIG.
    PhylomeDBiP77608.

    Enzyme and pathway databases

    UniPathwayiUPA00714.
    BioCyciEcoCyc:MHPDHYDROL-MONOMER.
    ECOL316407:JW0341-MONOMER.
    MetaCyc:MHPDHYDROL-MONOMER.
    BRENDAi4.2.1.80. 2165.
    SABIO-RKP77608.

    Miscellaneous databases

    EvolutionaryTraceiP77608.
    PROiP77608.

    Family and domain databases

    Gene3Di3.90.850.10. 1 hit.
    HAMAPiMF_01655. MhpD. 1 hit.
    InterProiIPR011234. Fumarylacetoacetase_C-rel.
    IPR023793. Keto_pentenoate-hydratase.
    [Graphical view]
    PfamiPF01557. FAA_hydrolase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56529. SSF56529. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMHPD_ECOLI
    AccessioniPrimary (citable) accession number: P77608
    Secondary accession number(s): P71205, P77045, Q2MC74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 15, 1998
    Last modified: November 2, 2016
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.