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Protein

3-(3-hydroxy-phenyl)propionate transporter

Gene

mhpT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Uptake of 3-(3-hydroxyphenyl)propionate (3HPP) across the cytoplasmic membrane. Transport is driven by the proton motive force. Does not transport benzoate, 3-hydroxybenzoate or gentisate.1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MHPT-MONOMER.
ECOL316407:JW5046-MONOMER.
MetaCyc:MHPT-MONOMER.

Protein family/group databases

TCDBi2.A.1.15.2. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
3-(3-hydroxy-phenyl)propionate transporter
Short name:
3HPP transporter
Alternative name(s):
3-(3-hydroxy-phenyl)propionate:H(+) symporter
Short name:
3HPP:H(+) symporter
Gene namesi
Name:mhpT
Synonyms:yaiK
Ordered Locus Names:b0353, JW5046
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13293. mhpT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence analysisAdd
BLAST
Transmembranei17 – 3721Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini38 – 5316PeriplasmicSequence analysisAdd
BLAST
Transmembranei54 – 7421Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini75 – 817CytoplasmicSequence analysis
Transmembranei82 – 10221Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini103 – 1053PeriplasmicSequence analysis
Transmembranei106 – 12621Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini127 – 14216CytoplasmicSequence analysisAdd
BLAST
Transmembranei143 – 16321Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini164 – 1641PeriplasmicSequence analysis
Transmembranei165 – 18521Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini186 – 21732CytoplasmicSequence analysisAdd
BLAST
Transmembranei218 – 23821Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini239 – 25315PeriplasmicSequence analysisAdd
BLAST
Transmembranei254 – 27421Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini275 – 2795CytoplasmicSequence analysis
Transmembranei280 – 30021Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini301 – 3066PeriplasmicSequence analysis
Transmembranei307 – 32721Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini328 – 33912CytoplasmicSequence analysisAdd
BLAST
Transmembranei340 – 36021Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini361 – 3699PeriplasmicSequence analysis
Transmembranei370 – 39021Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini391 – 40313CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion mutant loses the ability to utilize 3HPP at pH 8.2.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271E → A: Lack of 3HPP transport activity. 1 Publication
Mutagenesisi27 – 271E → D: Slight decrease in 3HPP transport activity. 1 Publication
Mutagenesisi75 – 751D → A or E: Lack of 3HPP transport activity. 1 Publication
Mutagenesisi272 – 2721A → H: 30% increase in 3HPP transport activity. 1 Publication
Mutagenesisi276 – 2761K → D: Lack of 3HPP transport activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4034033-(3-hydroxy-phenyl)propionate transporterPRO_0000050308Add
BLAST

Proteomic databases

PaxDbiP77589.
PRIDEiP77589.

Expressioni

Inductioni

Induced by 3HPP.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261625. 23 interactions.
STRINGi511145.b0353.

Structurei

3D structure databases

ProteinModelPortaliP77589.
SMRiP77589. Positions 41-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108SC4. Bacteria.
ENOG410YW8U. LUCA.
HOGENOMiHOG000114541.
InParanoidiP77589.
KOiK05819.
OMAiWLWISYF.
PhylomeDBiP77589.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P77589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRTPSSSS SRLMLTIGLC FLVALMEGLD LQAAGIAAGG IAQAFALDKM
60 70 80 90 100
QMGWIFSAGI LGLLPGALVG GMLADRYGRK RILIGSVALF GLFSLATAIA
110 120 130 140 150
WDFPSLVFAR LMTGVGLGAA LPNLIALTSE AAGPRFRGTA VSLMYCGVPI
160 170 180 190 200
GAALAATLGF AGANLAWQTV FWVGGVVPLI LVPLLMRWLP ESAVFAGEKQ
210 220 230 240 250
SAPPLRALFA PETATATLLL WLCYFFTLLV VYMLINWLPL LLVEQGFQPS
260 270 280 290 300
QAAGVMFALQ MGAASGTLML GALMDKLRPV TMSLLIYSGM LASLLALGTV
310 320 330 340 350
SSFNGMLLAG FVAGLFATGG QSVLYALAPL FYSSQIRATG VGTAVAVGRL
360 370 380 390 400
GAMSGPLLAG KMLALGTGTV GVMAASAPGI LVAGLAVFIL MSRRSRIQPC

ADA
Length:403
Mass (Da):41,551
Last modified:May 30, 2000 - v2
Checksum:i85EE921E57686FEB
GO

Sequence cautioni

The sequence AAB18077 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence D85613 differs from that shown. Reason: Frameshift at positions 105 and 163. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti295 – 2951L → V in D85613 (PubMed:9098055).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85613 Genomic DNA. No translation available.
U73857 Genomic DNA. Translation: AAB18077.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73456.2.
AP009048 Genomic DNA. Translation: BAE76135.1.
X97543 Genomic DNA. Translation: CAA66145.1.
PIRiA64763.
RefSeqiNP_414887.2. NC_000913.3.
WP_000107627.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73456; AAC73456; b0353.
BAE76135; BAE76135; BAE76135.
GeneIDi944997.
KEGGiecj:JW5046.
eco:b0353.
PATRICi32115843. VBIEscCol129921_0363.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85613 Genomic DNA. No translation available.
U73857 Genomic DNA. Translation: AAB18077.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73456.2.
AP009048 Genomic DNA. Translation: BAE76135.1.
X97543 Genomic DNA. Translation: CAA66145.1.
PIRiA64763.
RefSeqiNP_414887.2. NC_000913.3.
WP_000107627.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP77589.
SMRiP77589. Positions 41-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261625. 23 interactions.
STRINGi511145.b0353.

Protein family/group databases

TCDBi2.A.1.15.2. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP77589.
PRIDEiP77589.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73456; AAC73456; b0353.
BAE76135; BAE76135; BAE76135.
GeneIDi944997.
KEGGiecj:JW5046.
eco:b0353.
PATRICi32115843. VBIEscCol129921_0363.

Organism-specific databases

EchoBASEiEB3078.
EcoGeneiEG13293. mhpT.

Phylogenomic databases

eggNOGiENOG4108SC4. Bacteria.
ENOG410YW8U. LUCA.
HOGENOMiHOG000114541.
InParanoidiP77589.
KOiK05819.
OMAiWLWISYF.
PhylomeDBiP77589.

Enzyme and pathway databases

BioCyciEcoCyc:MHPT-MONOMER.
ECOL316407:JW5046-MONOMER.
MetaCyc:MHPT-MONOMER.

Miscellaneous databases

PROiP77589.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMHPT_ECOLI
AccessioniPrimary (citable) accession number: P77589
Secondary accession number(s): P77037, Q2MC71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: September 7, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.